BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0336.p (237 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311042|ref|YP_003775134.1| proline/glycine betaine ABC tra... 413 e-117 gi|300311044|ref|YP_003775136.1| proline/glycine betaine ABC tra... 113 1e-26 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 63 2e-11 gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate A... 54 8e-09 gi|300310541|ref|YP_003774633.1| nitrate transporter permease [H... 54 8e-09 >gi|300311042|ref|YP_003775134.1| proline/glycine betaine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 237 Score = 413 bits (1061), Expect = e-117, Method: Compositional matrix adjust. Identities = 228/237 (96%), Positives = 234/237 (98%) Query: 1 MTRVRHVFAGAFGFALVIAGLVWWIGPDVIQKYRGDLVYYTGKHLELVFSSMVLALATGI 60 MTRVRHVFAGA GFALV+AGLVWWIGPDVIQKYRGDL+YYTGKHLELVF+SM+LALATGI Sbjct: 1 MTRVRHVFAGALGFALVLAGLVWWIGPDVIQKYRGDLIYYTGKHLELVFTSMLLALATGI 60 Query: 61 PAGIALSRPGAQRSAEKFIQIFNIGNTIPSIAVLALALAAFGIGNGPTILALWLASLLPI 120 PAGIALSRPGAQRSAEKFIQIFNIGNTIPSIAVLALALA FGIGNGPTILALWLASLLPI Sbjct: 61 PAGIALSRPGAQRSAEKFIQIFNIGNTIPSIAVLALALAFFGIGNGPTILALWLASLLPI 120 Query: 121 VRNTYEGLKNVPAAMKEAARGIGMKPHQILFRVELPNALPIIVGGVRTSLAINVGTAPLS 180 VRNTYEGLKNVPAAMKEAARGIGMKPHQIL RVELPNALPIIVGGVRT+LAINVGTAPLS Sbjct: 121 VRNTYEGLKNVPAAMKEAARGIGMKPHQILLRVELPNALPIIVGGVRTALAINVGTAPLS 180 Query: 181 MLIGGESLGGLIFPGIYLNNHGQLLLGAAATALLALVLDALVTSLSGFYLARRGLRT 237 MLIGGESLGGLIFPGIYLNNHGQLLLGAAATALLALVLDALVT+LSGFYLARRGLRT Sbjct: 181 MLIGGESLGGLIFPGIYLNNHGQLLLGAAATALLALVLDALVTALSGFYLARRGLRT 237 >gi|300311044|ref|YP_003775136.1| proline/glycine betaine ABC transporters permease [Herbaspirillum seropedicae SmR1] Length = 216 Score = 113 bits (282), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 81/207 (39%), Positives = 125/207 (60%), Gaps = 10/207 (4%) Query: 28 DVIQKYRGD---LVYYTGKHLELVFSSMVLALATGIPAGIALSRPGAQRSAEKFIQIFNI 84 ++ Q + D ++ T +HL+LV ++ LA+A G+P GI + R + A + + I Sbjct: 2 NIFQYLQSDWPNILELTLQHLKLVGIAVSLAIAIGVPLGILIVR--VRWLAGPLMGLATI 59 Query: 85 GNTIPSIAVLALALAAF-----GIGNGPTILALWLASLLPIVRNTYEGLKNVPAAMKEAA 139 T+PSIA+ L + F GIG P I A++L SLLPI+RNTY L+N+ A +KEA Sbjct: 60 VLTLPSIALFGLMIPLFSKFGAGIGPLPAITAVFLYSLLPIMRNTYLALENIEAGIKEAG 119 Query: 140 RGIGMKPHQILFRVELPNALPIIVGGVRTSLAINVGTAPLSMLIGGESLGGLIFPGIYLN 199 GIGM Q L V+LP A+P+I+GGVRT++ +N+G ++ +IG LG LI I + Sbjct: 120 IGIGMTFWQRLRMVDLPLAVPVILGGVRTAVVMNIGVMAIAAIIGAGGLGVLILHAISQS 179 Query: 200 NHGQLLLGAAATALLALVLDALVTSLS 226 N +L++GA ++LA++ D L+ L Sbjct: 180 NMQKLVVGAVMISVLAIIADTLLQRLQ 206 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 62.8 bits (151), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 48/156 (30%), Positives = 76/156 (48%), Gaps = 10/156 (6%) Query: 88 IPSIAVLALALAAFGIGNGPTILALWLASLLPIVRNTYEGLKNVPAAMKEAARGIGMKPH 147 IP IA + LA+ FG+GN P + + L + P++ NT G++ V + AAR +G Sbjct: 122 IPPIAYIPLAILWFGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQS 181 Query: 148 QILFRVELPNALPIIVGGVRTSLAINVGTAPLSMLIG-----GESLGGLIFPGIYLNNHG 202 + RV LP A+P I+ GVR I +GTA + +++ LG I Sbjct: 182 TLFLRVMLPAAVPYILSGVR----IGIGTAFIVVIVAEMIAVSNGLGFRIMEAREYFWSD 237 Query: 203 QLLLGAAATALLALVLDALVTSLSGFYLA-RRGLRT 237 +++ G LL L +D ++ L+ + L RGL Sbjct: 238 KIIAGMITIGLLGLAIDLGMSRLNNYMLRWHRGLEN 273 >gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 54.3 bits (129), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 56/195 (28%), Positives = 91/195 (46%), Gaps = 11/195 (5%) Query: 35 GDLVYYTGKHLELVFSSMVLALATGIPAGIALSR--PGAQRSAEKFIQIFNIGNTIPSIA 92 G+L T L+++ + VL GI A AL+ Q + + ++ N +P+IA Sbjct: 82 GELFGKTWLSLQVLLKAYVL----GIVAAFALTTLAVSTQFGRDLLGTLTSMFNPLPAIA 137 Query: 93 VLALALAAFGIGNGPTILALWLASLLPIVRNTYEGLKNVPAAMKEAARGIGMKPHQILFR 152 +L +AL FG+G G I L + L + NTY G V ++ A R G++ + + Sbjct: 138 LLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTLRMAGRNYGLRGLGFVLQ 197 Query: 153 VELPNALPIIVGGVRTSLAINVGTAPLSMLIGGES-----LGGLIFPGIYLNNHGQLLLG 207 V +P ALP I+ G+R A T + L+ G + LG IF ++ G Sbjct: 198 VLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGWYIFQNRNELYTDKVFAG 257 Query: 208 AAATALLALVLDALV 222 A L+ L+++ LV Sbjct: 258 LAMVILIGLLVENLV 272 >gi|300310541|ref|YP_003774633.1| nitrate transporter permease [Herbaspirillum seropedicae SmR1] Length = 307 Score = 54.3 bits (129), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 45/155 (29%), Positives = 76/155 (49%), Gaps = 11/155 (7%) Query: 44 HLELVFSSMVLALATGIPAGIALSRPGAQRSAEKFIQIFNIGNTIPSIAVLALALAAFGI 103 L+ V + LA GIP G A+ R + A F I ++ + +A L + L F Sbjct: 116 SLQRVGTGFGLAALVGIPLGFAIGR--VEFLARMFGPIISLLRPVSPLAWLPIGLLVFKS 173 Query: 104 GNGPTILALWLASLLPIVRNTYEGLKNVPAAMKEAARGIGMKPHQILFRVELPNALPIIV 163 N I ++++ S+ P++ NT G++ VP AR + + ++L ++ P+ LP ++ Sbjct: 174 ANPAAIWSIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKVLTKILFPSVLPYML 233 Query: 164 GGVRTSLAINVGTAPL-----SMLIGGESLGGLIF 193 GVR S +GTA L ML GG +G ++ Sbjct: 234 TGVRLS----IGTAWLVIVAAEMLTGGVGIGFWVW 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.142 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 712,813 Number of extensions: 29338 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 12 Length of query: 237 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 155 Effective length of database: 1,231,600 Effective search space: 190898000 Effective search space used: 190898000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)