BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0306.p (572 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 1185 0.0 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 167 3e-42 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 128 1e-30 gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 94 4e-20 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 1185 bits (3066), Expect = 0.0, Method: Compositional matrix adjust. Identities = 566/572 (98%), Positives = 571/572 (99%) Query: 1 MSAEQLTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA 60 MSAEQLTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA Sbjct: 1 MSAEQLTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA 60 Query: 61 DAYSRSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDT 120 DAYSRSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDT Sbjct: 61 DAYSRSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDT 120 Query: 121 VGITRPCVKHNFLVKDVKDLAETVKKAFFIATTGRPGPVLVDIPKDITMHTCAYEYPKAV 180 VGITRPCVKHNFLVKDVKDLAETVKKAF+IATTGRPGPVLVDIPKDITMHTCAY YPKAV Sbjct: 121 VGITRPCVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAV 180 Query: 181 EMRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANASPELNKLVDRLGYPCTNT 240 EMRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANA+PELNKLVDRLGYPCTNT Sbjct: 181 EMRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNT 240 Query: 241 LMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFASNARK 300 LMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFAS+ARK Sbjct: 241 LMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARK 300 Query: 301 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDSAETRQNGAALDAWWKQINEWRK 360 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLD+AETRQNGAALDAWWKQINEWRK Sbjct: 301 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRK 360 Query: 361 RDCLKFATSDEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 RDCLKFATS+EFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG Sbjct: 361 RDCLKFATSEEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 Query: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG Sbjct: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 Query: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV Sbjct: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 Query: 541 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL 572 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL Sbjct: 541 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL 572 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 167 bits (422), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 146/557 (26%), Positives = 239/557 (42%), Gaps = 24/557 (4%) Query: 7 TGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRS 66 T ADIL+ L E GV +FG G A+ + DAI + + + I VRHE+ A AA ++ Sbjct: 4 TVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKL 63 Query: 67 SQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGITRP 126 + ++ V T+GPG + V GL A D P++ ISG VP+ G D QE + R Sbjct: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRD 123 Query: 127 CVKHNFLVKDVKDLAETVKKAFFIATTGRPGPVLVDIPKDITMHTCAYEYPKAVEMRSYK 186 ++ ++ +A A R G ++IP D+ + P +R Sbjct: 124 VAAYSQIITSPDQAPAVFHQAIAQAYAQR-GVAHLNIPADVIGAKTSGSVPSLHTLRERT 182 Query: 187 PVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANASPELNKLVDRLGYPCTNTLMGLGA 246 P+ + I+ A L+ +A I+ G G A E+ L +L P +T G Sbjct: 183 PLSP-PAADIQAAADLINAAGSVAIFAGNGC--RGAIDEVLALASKLQAPVMHTFRGKDL 239 Query: 247 YRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFASNARKIIHIDI 306 ++G G+ G A+ +V++ +G+ + F K+I ID Sbjct: 240 ASYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPY------SEFLPTKSKVIQIDE 293 Query: 307 DPSSISKRVKVDIPIVGNVKDVLQELLSQLDSAETRQNGAALDAWWKQINEWRKRDCLKF 366 + +R V + I G+V +Q+LL ++ E + +GA L +Q N R+R K Sbjct: 294 RGFVLGRRAPVTLGITGSVAPAVQQLLERV---EGKTDGAFL----QQANAHRERWNAKL 346 Query: 367 ATSDEF------IKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 ++ E IKPQSV + + ++ DA D G +W + +R + S Sbjct: 347 DSAAEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFN 406 Query: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 +G L A G+Q + + V G+ M + E T ++ L K+++ NNR G Sbjct: 407 NAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWG 466 Query: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 +V + + G E PDF A + G G + P +++G L++ A V Sbjct: 467 LV-HLEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAV 525 Query: 541 FMNFITDQTENVWPMVK 557 FI V P +K Sbjct: 526 LDVFIDPDELPVMPHIK 542 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 128 bits (322), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 142/577 (24%), Positives = 247/577 (42%), Gaps = 73/577 (12%) Query: 20 GVEHVFGYPG----GAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSQKVGVAIV 75 GV +FG+ G LY Y A + +EQA H+A AY+++ + + V Sbjct: 40 GVFSIFGHGNVAGMGEALYQYRATLPTYR-----AHNEQAMAHSAIAYAKAHLRQRMMAV 94 Query: 76 TS--GPGVTNAVTGLATAYMDSIPMVVISGQV-----PSTAIGQDAFQECDTVGIT---- 124 T+ GPG TN +T A A+++ +P++++ G V P + Q ++ + G++ Sbjct: 95 TTSIGPGATNLLTAAALAHVNRLPVLLLPGDVFVSRRPDPVLQQ--LEDFNDPGLSVNDA 152 Query: 125 -RPCVKHNFLVKDVKDLAETVKKAFFIAT-TGRPGPVLVDIPKDITMHTCAYEYP----- 177 RP + + + L + +A + T + GPV + +P+D+ T AY+YP Sbjct: 153 FRPLSRMFDRICYPEQLLTALPRAIQVLTDPAQCGPVTLALPQDV--QTMAYDYPLDFFS 210 Query: 178 -KAVEMRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANASPELNKLVDRLGYP 236 + + R+ P + ++ A+ LL A +P++ GGG++ NA L + ++ G P Sbjct: 211 PRVIVPRAQAPAAQ----ELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVP 266 Query: 237 CTNTLMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFAS 296 T G A S +G G+ G+ AN Q DV+IA+G R D G+ FA Sbjct: 267 VAETQAGKSALPWSHALQMGAIGVTGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQ 326 Query: 297 NARKIIHIDIDPSSISKRVKVDIPI-VGNVKDVLQELLSQLDSAETRQNGAALDAWWKQI 355 ++++++++ K + + G + L L SA A A Sbjct: 327 A--RLLNLNVNAMDAHKWRGLALQADAGLGLEALSHALEGWRSAPEWHARAHTLA----- 379 Query: 356 NEWRKRDCLKFATSD---------EFIKPQSVVQKVWEVTKGDAFI----TSDVGQHQMW 402 +WR+R +D E I ++ + V + D + T H++W Sbjct: 380 QDWRERVAQITGQTDTGGRLPYDGEVIG--AIQRSVADSPSQDIVVCAAGTLPAELHKLW 437 Query: 403 AAQYYGFDKPRRWINSGGLGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATC 462 A +P + G MG + +GV++A P V I G+ S M ELAT Sbjct: 438 RA-----GRPGAYHVEYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDGSYLMMNSELATS 492 Query: 463 KQYHLTPKIILLNNRFLGMVRQWQQIDYGSRYSESYMDSLP--------DFNKLAESYGH 514 +++L+NR G + + QQ G+ ++ D L DF A S G Sbjct: 493 VMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGA 552 Query: 515 VGMKIEKPGDVDGALREAFAMKDRLVFMNFITDQTEN 551 +G ++ +++ AL+ A A DR + TD + Sbjct: 553 LGENVKTITELEAALQRARA-ADRSYLVCIDTDASRT 588 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 93.6 bits (231), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 130/531 (24%), Positives = 217/531 (40%), Gaps = 36/531 (6%) Query: 20 GVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSQKVGVAIVTSGP 79 G+ VF PG L ++ F +D F+++L E V AD ++++S + + S Sbjct: 23 GMTSVFANPGSTELPMFRD-FPED-FRYVLGLQEAVVVGMADGFAQASGNAALVNLHSAA 80 Query: 80 GVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAF-QECDTVGITRPCVKHNFLVKDVK 138 GV NA+ + TA+ + P+++ +GQ + + D F + +P +K + Sbjct: 81 GVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKPYLKWSIEPARAA 140 Query: 139 DLAETVKKAFFIATTGRPGPVLVDIPKDITMHTCAYEYPKAVEMRSYKPVDKGHSGQIRK 198 D+ + +A+ +A GPV V IP D P V R +P + Sbjct: 141 DVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPTQVTSRC-RPEPAAIARLAAA 199 Query: 199 ALQLLLSAERPMIYTGGGIILANASPELNKLVDRL-GYPCTNTLMGLGAYRSSSDKFVG- 256 Q ++RP I G + A A L + L + ++ F G Sbjct: 200 LEQ----SQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMSSRCSFPEDHPLFAGF 255 Query: 257 MPGMHGTYEANMAMQHCDVLIAIGA-RFDDRVIGNPKHFASNARKIIHIDIDPSSISKRV 315 +P M + A+Q D+L A+GA F V G+ H A ID DP + S Sbjct: 256 LPAMR--EKIVQALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQLID-DPVTASW-T 311 Query: 316 KVDIPIVGNVKDVLQELLSQLDSAETRQNGAALDAWWKQINEWRKRDCLKFATSDEFIKP 375 IVGN++ L +LL+Q+ Q + E + +A Sbjct: 312 PTGQAIVGNIQLGLDDLLAQIRRQPPTQRAQPPARPARARVEASAPLSMAYA-------- 363 Query: 376 QSVVQKVWEVTK-GDAFITSDVGQHQMWAAQYYGFDKPRRW--INSGGLGTMGVGLPYAM 432 +Q + +V + GD + G + A + F +P + + SGGL G G+P A+ Sbjct: 364 ---LQTLADVREAGDIVVEEAPGSRPVMQA-HLPFTRPGSFYTMASGGL---GYGMPAAV 416 Query: 433 GVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMVRQWQQIDYGS 492 GV +A P + V + G+ S Q + + Q L ++LNN ++ + + Sbjct: 417 GVALARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPVFGFG 476 Query: 493 RYSESYMDSLP--DFNKLAESYGHVGMKIEKPGDVDGALREAFAMKD-RLV 540 + LP DF LA S G G+++ + + ALREA + RLV Sbjct: 477 PEDPVHGTELPGLDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLV 527 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,788,235 Number of extensions: 74582 Number of successful extensions: 223 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 5 Length of query: 572 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 482 Effective length of database: 1,193,720 Effective search space: 575373040 Effective search space used: 575373040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)