BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0299.p (165 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311071|ref|YP_003775163.1| hypothetical protein Hsero_1742... 187 5e-49 >gi|300311071|ref|YP_003775163.1| hypothetical protein Hsero_1742 [Herbaspirillum seropedicae SmR1] Length = 168 Score = 187 bits (474), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 114/168 (67%), Positives = 136/168 (80%), Gaps = 6/168 (3%) Query: 1 MNMAITSSTSTLTTLGSLDPYGL---GSSGTSNPVTAASNPAAVAQAVELSSEASVIVTL 57 MNMAI +S+ LG+LDPYGL +S ++ V A ++PAAVAQAVEL+++ASV+V+L Sbjct: 4 MNMAIDTSS---IPLGNLDPYGLPGNSASAGNSAVKAVNDPAAVAQAVELAAQASVVVSL 60 Query: 58 GGGSSANSGLTYNAAGLFDSIVNAGSSAGTSTDLTQNQATQSYYQGVLGSIVTPGATSGI 117 GG SS N LTYNAAGLFD+IV AGS+ G S DLTQNQATQSYYQG+LGSIV P + G+ Sbjct: 61 GGASSGNGALTYNAAGLFDAIVGAGSADGASPDLTQNQATQSYYQGILGSIVIPSSNDGV 120 Query: 118 YDGSGVFAGEGLSSQLSLLLKSQPDLTGSIVEDIVNQNVVGGLISTMA 165 YDGSG F+G+ LSSQLSLLLKS PDLTG+IVEDIVNQ +VGGLIST A Sbjct: 121 YDGSGQFSGQALSSQLSLLLKSNPDLTGAIVEDIVNQGIVGGLISTTA 168 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.305 0.123 0.321 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 364,790 Number of extensions: 12665 Number of successful extensions: 53 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 1 Length of query: 165 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 87 Effective length of database: 1,250,540 Effective search space: 108796980 Effective search space used: 108796980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (21.9 bits) S2: 127 (53.5 bits)