BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0292.p (307 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 489 e-139 gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 123 2e-29 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 94 2e-20 gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum se... 64 1e-11 gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 60 2e-10 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 489 bits (1260), Expect = e-139, Method: Compositional matrix adjust. Identities = 258/303 (85%), Positives = 276/303 (91%) Query: 1 MNKPILAYGEAMVEFNQRIDTPRQYLQGFGGDTSNFAIAAARQGAASGYVSAVGDDHFGQ 60 M+K ILAYGEAMVEFNQRID PRQ+LQG+GGDTSNFAIAAARQGAAS Y+SAVGDDHFGQ Sbjct: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQ 60 Query: 61 DLLALWQREQVDVQHVAVQANGSTGLYFVTHDDQGHHFHYRRAGSAASCYTVGDLPLQAI 120 DLLALWQRE+VDV+HVA+ GSTG+YFVTHD GHHFHYRRAGSAAS Y LPL+AI Sbjct: 61 DLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHHFHYRRAGSAASAYQAKALPLEAI 120 Query: 121 AAAGALHLSGISLAISESACDAGLAAMAHARAAGVTTSLDTNLRLRLWPLERAQAKMAEA 180 AAAGALHLSGISLAISESAC+AGLAAMAHARA+GVTTSLDTNLRLRLWPLERA+A+M EA Sbjct: 121 AAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEA 180 Query: 181 FRLCDVCLPSWEDVSMLTGLNDRDAIVDHLLDYGVKLVAFKLGHEGCYVATRQERRMVSP 240 FRLCD+CLPSWEDVS+LTGL DRDAIVD LL YGVKLVAFKLG EGCYVAT +RRMV+P Sbjct: 181 FRLCDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQRRMVAP 240 Query: 241 YPVQALDATGAGDCFGGAFMAELMAGRDPFQAARYANVAAALSTTGYGAVEPIPHRDLVE 300 YPV +LDATGAGDCFGGAFMAELMAGRD F AARYANVAAALSTTGYGAVEPIP R VE Sbjct: 241 YPVDSLDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQVE 300 Query: 301 AAL 303 A L Sbjct: 301 AVL 303 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 100/310 (32%), Positives = 136/310 (43%), Gaps = 8/310 (2%) Query: 1 MNKPILAYGEAMVEFNQRIDTP----RQYLQGFGGDTSNFAIAAARQGAASGYVSAVGDD 56 M ++ +GEA+ P +Y + G +N AI AR G G+ S VG+D Sbjct: 3 MQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 Query: 57 HFGQDLLALWQREQVDVQHVAVQANGSTGLYFVTHDDQGHH--FHYRRAGSAASCYTVGD 114 FG+ + +E V+V V T + G Y R GSAAS +V D Sbjct: 63 AFGRFIRQRVAQEGVEVSRVITDMEFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSVDD 122 Query: 115 LPLQAIAAAGALHLSGISLAISESACDAGLAAMAHARAAGVTTSLDTNLRLRLWPLERAQ 174 AA LH +GI+ A+S + AM R G T S D NLR LWP + Sbjct: 123 FDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQEVM 182 Query: 175 AKMAEAFRL-CDVCLPSWEDVSMLTGLNDRDAIVDHLLDYGVKLVAFKLGHEGCYVATRQ 233 A+ A D LP + +LTG +D I L+ GVKLV KLG EG Y Q Sbjct: 183 AQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRNGQ 242 Query: 234 ERRMVSPYPV-QALDATGAGDCFGGAFMAELMAGRDPFQAARYANVAAALSTTGYGAVEP 292 V+ PV + +D GAGD F ++ ++ G QA N A + G +E Sbjct: 243 GEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDMEG 302 Query: 293 IPHRDLVEAA 302 +P R +EAA Sbjct: 303 LPTRAELEAA 312 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 93/280 (33%), Positives = 129/280 (46%), Gaps = 24/280 (8%) Query: 25 YLQGFGGDTSNFAIAAARQGAASGYVSAVGDDHFGQDLLALWQREQVDVQHVAVQANGST 84 + G GG SN A+AAAR GA ++SA+G D FG L+LW+RE + HV V T Sbjct: 39 FAMGPGGKGSNQAVAAARVGAPVRFISAIGKDAFGDFALSLWRRETI-APHVRVIDGAPT 97 Query: 85 GLYFVTHDDQGHHFHYRRAGSAASCYTVGDL--PLQAIAAAGALHLSGISLAISESACDA 142 G F+ +D AAS + D+ QAI AA + + E +A Sbjct: 98 GAAFIYVNDASGDNAIIVVPGAASQLSEQDVERERQAIEAA------KVFVTQLEQPPEA 151 Query: 143 GLAAMAHARAAGVTTSLDTNLRLRLWPLERAQAKMAEAFRLCDVCLPSWEDVSMLTG--- 199 LA + ARAAG TT + L A A+ + LCD P+ + ++LTG Sbjct: 152 ALAGLQIARAAGTTTVFNPAPAL---------AFPAQVYGLCDFITPNEHEAALLTGIEI 202 Query: 200 --LNDRDAIVDHLLDYGVKLVAFKLGHEGCYVATR-QERRMVSPYPVQALDATGAGDCFG 256 ++D D LL+ GV LG +G + +R Q + + ++ GAGD F Sbjct: 203 HHIDDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLPALCAGTVVETAGAGDAFN 262 Query: 257 GAFMAELMAGRDPFQAARYANVAAALSTTGYGAVEPIPHR 296 GAF A L G +AAR A AA+S T G +P R Sbjct: 263 GAFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMPSR 302 >gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 85/278 (30%), Positives = 120/278 (43%), Gaps = 24/278 (8%) Query: 30 GGDTSNFAIAAAR---QGAASGYVSAVGDDHFGQDLLALWQREQVDVQHVAVQANGSTGL 86 GG +N A+A AR G V+ VGDD FG + +D +++ A +TG+ Sbjct: 36 GGKGANQAVACARLAAPGTRVAMVACVGDDAFGGQMRQSITACGIDDRYIDEVAGEATGI 95 Query: 87 YFVTHDDQGHHFHYRRAGSAASCYTVGDLPLQAIAAAGAL-HLSGISLAISESACDAGLA 145 + D + AG+ G L ++ I A AL + I L E + Sbjct: 96 ASIMVDANAQNSIVIAAGAN------GRLDVERIERARALIEQASIVLLQLEVPMATVIH 149 Query: 146 AMAHARAAGVTTSLDTNLRLRLWPLERAQAKMAEAFRLCDVCLPSWEDVSMLTGLNDRDA 205 ++ A A G T L+ AQA + D + + + +ML D Sbjct: 150 SIELAHALGKTVVLNP---------APAQALPRALLQKIDYLILNEIEAAMLAEEQSEDI 200 Query: 206 --IVDHLLDYGVKLVAFKLGHEGCY--VATRQERRMVSPYPVQALDATGAGDCFGGAFMA 261 + L D G + V LG +G Y A Q+R + + VQA+D T AGD F G F+ Sbjct: 201 PMLARKLHDLGARNVVVTLGEKGVYGSFADGQQRHLPA-RKVQAVDTTAAGDTFIGGFIG 259 Query: 262 ELMAGRDPFQAARYANVAAALSTTGYGAVEPIPHRDLV 299 + GRD F+A YA AAALS T GA IP RD V Sbjct: 260 AIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIPTRDEV 297 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 76/307 (24%), Positives = 116/307 (37%), Gaps = 31/307 (10%) Query: 15 FNQRIDTPRQYLQGF----GGDTSNFAIAAARQGAASGYVSAVGDDHFGQDLLALWQREQ 70 + Q++ P + F GG ++N A AR G S ++ +GD+ GQ L A E Sbjct: 34 YAQQVGAPLSDVSSFAKYLGGSSANIAFGCARLGLKSAMLARIGDEANGQFLKAELAAEG 93 Query: 71 VDVQHVAVQANGSTGLYFV-THDDQGHHFHYRRAGSAASCYTVGDLPLQAIAAAGALHLS 129 DV HV V T L + D + R A D+ IA++ AL ++ Sbjct: 94 CDVSHVTVDPQRLTALVMLGLKDKDSFPLIFYRDNCADMALAPEDIDEAFIASSKALLIT 153 Query: 130 GISLAISESACDAGLAAMAHARAAGVTTSLDTNLRLRLWPL-------------ERAQAK 176 G + + + L A+ +AR V T LD + R LW L + Sbjct: 154 GTHFSTPQVHRSSSL-ALQYARRHNVRTVLDIDYRPVLWGLTSKGDGETRFIASDGVTQH 212 Query: 177 MAEAFRLCDVCLPSWEDVSMLTGLNDRDAIVDHLLDYGVKLVAFKLGHEGCYV--ATRQE 234 + D+ + + E+ + G D A + + + K G GC V E Sbjct: 213 LQGILPQFDLVVGTEEEFMIAGGSTDIMAALRAVRALCAATLVVKRGPLGCVVLHGAIPE 272 Query: 235 R-------RMVSPYPVQALDATGAGDCFGGAFMAELMAGRDPFQAARYANVAAALSTTGY 287 R R V V+ L+ GAGD F F+ + D RYAN AL + + Sbjct: 273 RLDDGFHYRGVQ---VEVLNVLGAGDAFISGFLKGWLNEEDDEHCCRYANACGALVVSRH 329 Query: 288 GAVEPIP 294 +P Sbjct: 330 ACAPAMP 336 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 929,466 Number of extensions: 36493 Number of successful extensions: 109 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 106 Number of HSP's successfully gapped: 5 Length of query: 307 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 222 Effective length of database: 1,217,395 Effective search space: 270261690 Effective search space used: 270261690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)