BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0289.p (498 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [H... 924 0.0 gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [H... 437 e-123 gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [H... 163 3e-41 >gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 496 Score = 924 bits (2387), Expect = 0.0, Method: Compositional matrix adjust. Identities = 446/498 (89%), Positives = 469/498 (94%), Gaps = 2/498 (0%) Query: 1 MTSTLQSSGSPRHCDILIRHALVIDGSGAEPVQADVAVRNGRIVAVGQLAGWQAEQEIDG 60 M ST ++ R CDILIRHALVIDGSGAEPV ADVAV GR+ A+G+L WQA QEID Sbjct: 1 MLSTTHAADGERRCDILIRHALVIDGSGAEPVAADVAVHKGRVAAIGKLDDWQAAQEIDA 60 Query: 61 TARVLSPGFIDAHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGDPPDPMN 120 + RVLSPGFID HTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKG+PPDPMN Sbjct: 61 SGRVLSPGFIDVHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGEPPDPMN 120 Query: 121 LLGKSEAFHYPSFASYVEAVNAAQPAINVAALVGHTALRNNHMDRLDRAASDAEIDGXNV 180 LLGK EAFHYPSFASYVEAVNAA+PAINVAALVGHTALRNNHMDRLDRAA+++EI G + Sbjct: 121 LLGKREAFHYPSFASYVEAVNAARPAINVAALVGHTALRNNHMDRLDRAATESEIAG--M 178 Query: 181 YAQLREALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILNA 240 AQLREALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADIL+A Sbjct: 179 RAQLREALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILDA 238 Query: 241 MDEAFRIGKHARVPVVISHLKCAGVENWGRSSEVLRALEAASRHQHVGCDCYPYTASSST 300 M+E+FRIGKHARVPVVISHLKCAGVENWGRSSEVL ALE A+RHQHVGCDCYPYTASSST Sbjct: 239 MEESFRIGKHARVPVVISHLKCAGVENWGRSSEVLHALERAARHQHVGCDCYPYTASSST 298 Query: 301 LDLKQVTADYDIQVTWSEPHPEQGGKMLKQIAADWGVDLHAAAARLMPAGAVYHCMSDDD 360 LDLKQVT DYDIQVTWSEPHPEQGGKMLKQIAA+W VDLHAAAARLMPAGAVYHCMSDDD Sbjct: 299 LDLKQVTGDYDIQVTWSEPHPEQGGKMLKQIAAEWDVDLHAAAARLMPAGAVYHCMSDDD 358 Query: 361 VNRILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQ 420 VNRILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQ Sbjct: 359 VNRILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQ 418 Query: 421 RFGLEQRGFVREGYHADLVLFDPETIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQS 480 RFGL+ RGFVR GYHADLVLFD ET+RDAASFTDPMQPAHGIEAVWVNG+LAYRGQDK++ Sbjct: 419 RFGLDLRGFVRVGYHADLVLFDAETVRDAASFTDPMQPAHGIEAVWVNGELAYRGQDKKA 478 Query: 481 TSARAGRFLARTVQKTSA 498 T+ARAGRFLARTVQKTSA Sbjct: 479 TAARAGRFLARTVQKTSA 496 >gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 498 Score = 437 bits (1123), Expect = e-123, Method: Compositional matrix adjust. Identities = 231/479 (48%), Positives = 311/479 (64%), Gaps = 8/479 (1%) Query: 15 DILIRHALVIDGSGAEPVQADVAVRNGRIVAVGQLAGWQAEQEIDGTARVLSPGFIDAHT 74 D+LI +IDGS A +AD+ VR+GRI +G L+ QA Q ID + ++++PGFID+HT Sbjct: 8 DLLITGGTIIDGSKAPRFRADIGVRHGRIAEIGNLSHRQAAQRIDASGKIVAPGFIDSHT 67 Query: 75 HDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGDPPDPMNLLGKSEAFHYPSFA 134 HDD V+ P+M K+SQGVTTV+ GNCGIS AP+ P P++LL ++ + +F+ Sbjct: 68 HDDQAVLSQPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADGTRFETFS 127 Query: 135 SYVEAVNAAQPAINVAALVGHTALRNNHMDRLDRAASDAEIDGXNVYAQLREALEHGALG 194 SY++A+ A+NVAA+VGH+ LR MD L+R A EI N A L EALE GA+G Sbjct: 128 SYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMN--ALLDEALEAGAIG 185 Query: 195 LSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILNAMDEAFRIGKHARVP 254 +STG Y A +A T+EV+ + P+A G LY TH+R E + A++E+F IG+ P Sbjct: 186 ISTGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSMEALEESFLIGRELNAP 245 Query: 255 VVISHLKCAGVENWGRSSEVLRALEAASRHQHVGCDCYPYTASSSTL--DLKQVTADYDI 312 VVISH K N+G+SS+ L + A + Q V DCYPY ASS+ L D K++ I Sbjct: 246 VVISHHKLQRAHNFGKSSQTLARIRQAMQCQCVALDCYPYNASSTMLHRDPKRLVGR--I 303 Query: 313 QVTWSEPHPEQGGKMLKQIAADWGVDLHAAAARLMPAGAVYHCMSDDDVNRILSHPATVV 372 ++ S+PHPEQ G+ L IAA+WGV AAARL PA AVY M + DV IL+ T++ Sbjct: 304 LISSSKPHPEQAGRELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDETMI 363 Query: 373 GSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLEQRGFVRE 432 GSDG+P PHPRLWG FPR+LG+YSR+ LF L AV KMTGL+A+ F L +RG + Sbjct: 364 GSDGIPTGEKPHPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGTLEV 423 Query: 433 GYHADLVLFDPETIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQSTSARAGRFLAR 491 G++AD+V+FD TIRD AS+ P +PA GI+AV VNG + +R Q ARAG +AR Sbjct: 424 GHYADIVVFDAATIRDTASYETPAEPAQGIDAVIVNGVVTWRL--GQHMGARAGHVIAR 480 >gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 163 bits (413), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 143/529 (27%), Positives = 239/529 (45%), Gaps = 79/529 (14%) Query: 11 PRHCDILIRHALVIDGSGAEPVQADVAVRNGRIVAVGQL--AGWQAEQEIDGTARVLSPG 68 P D++I+ + DG EP+ ++ + RIV VG+ + + A + ID +++PG Sbjct: 45 PVQLDLIIQSDRIYDGEHDEPLSGELGIAGDRIVYVGKADRSRFTALRVIDARGAIVAPG 104 Query: 69 FIDAHTHDD----TNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGDPPDPMNLLGK 124 FIDAHTH D T+ I+ ++ L+QGVTT V+GN G G Sbjct: 105 FIDAHTHADRDMFTDDIKKRQVDYFLTQGVTTAVIGNDG------------------GGS 146 Query: 125 SEAFHYPSFASYVEAVNAAQPAINVAALVGHTALRNNHMDRLDRAASDAEIDGXNVYAQL 184 E ++ S + P N A VG +R + + D+ +++ A + Sbjct: 147 YEVAQQAAWLSR----HGIGP--NAAMYVGFGPIRRSQLGEADQQPDQKQLEAMK--ALV 198 Query: 185 REALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFA---DILNAM 241 + + GA+G STGL Y A T EV+ALA+ A++GG+Y +H+R E + + ++ Sbjct: 199 AKGMCEGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIYDSHIRDEASYNVGLAESI 258 Query: 242 DEAFRIGKHARVPVVISHLKCAGVENWGRSSEVLRALEAA-SRHQHVGCDCYPYTASSST 300 E RI + A + ++H+K G G+S ++R +EAA ++ + D YP+ ASS+ Sbjct: 259 REVIRIAREAGIAANVAHIKALGKSVEGQSGAIIRMIEAARAQGLRITADQYPWLASSTR 318 Query: 301 LD-----------------------LKQVTADYDI-----------QVTWSEPHPEQGGK 326 +Q DI + +S+ +P+ GK Sbjct: 319 FSAALIPPWALDGGRPAMLRRFDDPAQQQRLHRDIGENLRLRNGAGAILFSQGNPKYVGK 378 Query: 327 MLKQIAADWGVDLHAAAARLMPAGAVY---HCMSDDDVNRILSHPATVVGSDGLPNDPLP 383 L+Q+A + D +AA ++ + S+ DV + P + SD Sbjct: 379 TLEQLAQERNTDPVSAAIEVLRDSEIVIASFNQSNKDVVAFMKRPWVMTSSDSFHG---- 434 Query: 384 HPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLEQRGFVREGYHADLVLFDP 443 HPR +F Y R Q +LA + T A G+ +RG ++ G+ AD+V+FDP Sbjct: 435 HPRTVASFAEKYHEYVRNQHTITLAQFIRSSTSYPADALGIARRGRLQPGHFADVVVFDP 494 Query: 444 ETIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQSTSARAGRFLART 492 A++ + + + G+ A VNG+L + + T+A GR L RT Sbjct: 495 IAYAPRATYVNNEERSVGVVATVVNGELVV--DEGRLTNAMPGRPLLRT 541 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,646,795 Number of extensions: 72173 Number of successful extensions: 258 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 252 Number of HSP's successfully gapped: 5 Length of query: 498 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 409 Effective length of database: 1,198,455 Effective search space: 490168095 Effective search space used: 490168095 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)