BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0273.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 640 0.0 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 139 5e-34 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 125 5e-30 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 90 2e-19 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 67 3e-12 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 640 bits (1650), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/325 (96%), Positives = 322/325 (99%) Query: 1 MRAIEIKQFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 MRAIEIK+FGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 Query: 61 PGLEVAGEIVAGDLGDSGFKLGDLVCALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAAL 120 PGLEVAGEIVAGDLGDSGFKLGDLVCALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAAL Sbjct: 61 PGLEVAGEIVAGDLGDSGFKLGDLVCALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAAL 120 Query: 121 PENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSADKCRAC 180 PENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSA+KCRAC Sbjct: 121 PENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKCRAC 180 Query: 181 EELGAERAINYKTEDFEAVVKELTGGKGVDVILDMVGGDYLPREIKSLADDGRLVFIAQL 240 EELGAERAINYKTEDFEAVVKELTGG+GVDVILDMVGGDYLPREIK+LADDGRLVFIAQL Sbjct: 181 EELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLVFIAQL 240 Query: 241 GGSKGELDMGQVMRRRLTITGSTLRPRPIAFKAAIAANLRQHVWPLIEAGKIKPVIHHRF 300 GG+KG+LDMGQVMRRRLTITGSTLRPRP+AFKAAIAA LR+HVWPLIEAGKI+PVIHHRF Sbjct: 241 GGAKGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLREHVWPLIEAGKIRPVIHHRF 300 Query: 301 PLEQAAQAHAMMESSAHIGKIMLEL 325 PLEQAAQAHAMME S HIGKIMLEL Sbjct: 301 PLEQAAQAHAMMEGSTHIGKIMLEL 325 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 139 bits (349), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 113/330 (34%), Positives = 156/330 (47%), Gaps = 18/330 (5%) Query: 2 RAIEIKQFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDLP 61 +AI + + GGPEV++ + V E AGEV I+ A G+N D + R G YP P LP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQP-----LP 57 Query: 62 ---GLEVAGEIVAGDLGDSGFKLGDLVC-ALVQGGGYAEFCAAPLAQCLPVPKGLSALEA 117 G E AG I A G SGFK+GD V A G YAE P + +P +S A Sbjct: 58 AGLGQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTA 117 Query: 118 AALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSADKC 177 AA+ TV + + +T+L + GIG+ A Q ARALG + T S +K Sbjct: 118 AAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKA 177 Query: 178 RACEELGAERAINYKTEDFEAVVKELTGGKGVDVILDMVGGDYLPREIKSLADDGRLVFI 237 + GA INYKTE+F VKELTGG+GV V+ D +G D + L G +V Sbjct: 178 ALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSF 237 Query: 238 AQLGGSKGELDMGQVMRRRLTITGSTLRPRP-IAFKAAIAANLRQ---HVWPLIEAGKIK 293 G + ++ R GS RP + + A L ++ ++ KI Sbjct: 238 GNASGPVAPFGVSELASR-----GSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKIS 292 Query: 294 PVIHHRFPLEQAAQAHAMMESSAHIGKIML 323 I+ R+ L+ AQAHA +E G +L Sbjct: 293 IEINQRYALKDVAQAHADLEGRKTTGSTIL 322 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 125 bits (314), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 111/355 (31%), Positives = 175/355 (49%), Gaps = 52/355 (14%) Query: 1 MRAIEIKQFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASD- 59 M+AI+ FGGPEVL+ + PVPEL+ GEVL++VHA G+N PD + R G +PP Sbjct: 6 MKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPT 65 Query: 60 -----LPGLEVAG--EIVAGDLGDSGFKLGDLVCALVQ------GG--GYAEFCAAPLAQ 104 + G +++G E VA D+ D F +GD V ++V+ GG YA++ + ++ Sbjct: 66 VAFPLILGTDISGVVEAVADDVKD--FAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASE 123 Query: 105 CLPVPKGLSALEAAALPENYFTVWSNVFDRGQ--------------LSGEETLLVQGGSS 150 P+ +S +AAA P + T W + + G ++T++V G + Sbjct: 124 IALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAG 183 Query: 151 GIGVTAIQIARALGHRVFATAGSADKCRACEELGAERAINYKTEDFEAVVKELTGGKGVD 210 G+G A+Q+A+ G RV A GS +LGA+ I+Y E V+ + VD Sbjct: 184 GVGHFALQLAKWKGARVIAI-GSGGHEAFLRDLGADEFIDYTRSAAEDVLSD------VD 236 Query: 211 VILDMVGGDYLPREIKSLADDGRLVFIAQLGGSKGELDMGQVMRRRLTITGSTLRPRPIA 270 +++D VGG R +++L G L I LG S E RL + ST + R Sbjct: 237 LVIDAVGGPASGRFLRTLKRGGALFPIFPLGFSGAE------EAARLGVNVSTTQVR--- 287 Query: 271 FKAAIAANLRQHVWPLIEAGKIKPVIHHRFPLEQAAQAHAMMESSAHIGKIMLEL 325 + A L+Q + L+++G ++ I F L+ A +AH GKI+L + Sbjct: 288 ---SSGAQLQQ-LAGLLDSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSV 338 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 89/332 (26%), Positives = 149/332 (44%), Gaps = 31/332 (9%) Query: 1 MRAIEIKQFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTG--NYPVPPGAS 58 MRAI Q+G P VL C+ L G V + + AAGV D R G + V Sbjct: 1 MRAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLP 60 Query: 59 DLPGLEVAGEIVAGDLGDSGFKLGDLVCALVQGGGYAEFCAA---PLAQCLPVPKGLSAL 115 +PG + AG I A G ++G+ VC + G A P Q + P LS Sbjct: 61 KVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVLSMQ 120 Query: 116 EAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAD 175 +AAAL + + W + ++ + + GS +G +Q+A LG V A+ S++ Sbjct: 121 QAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSSN 180 Query: 176 KCRACEELGAERAINYKTEDFEAVVKELTGGKGVDVILDMVGGDYLPREIKSLADDGRLV 235 LGA R + Y ++ V + DV++D+VGG + + L G+LV Sbjct: 181 -VEYVAALGASRVLAYDRDELGRVRDQ-------DVVIDLVGGAAHDQSYRMLRRGGQLV 232 Query: 236 F-IAQLGGSKGELDMGQVMRRRLTITGSTLRPRPIAFKAAIAANLRQHVWPLIEAGKIKP 294 + +A +GE + G +RR L + ++ + +AA +Q VW +P Sbjct: 233 WLVAAPIRERGE-EFGVSVRRALIVEDQSILQQ-------VAALAQQGVW--------RP 276 Query: 295 VIHHRFPLEQAAQAHAMMES-SAHIGKIMLEL 325 + P+++ A+AH +E+ G+++L++ Sbjct: 277 AVSGVLPMQEIARAHQRIEAGEVTRGRLVLDI 308 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 66.6 bits (161), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 65/234 (27%), Positives = 101/234 (43%), Gaps = 32/234 (13%) Query: 1 MRAIEIKQFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 M+A +++FG P ++ + PVP G++L+K A+GV D+ G++PV P + Sbjct: 5 MKAAVVREFGKPLSIE--QVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFI 62 Query: 61 PGLEVAGEIVAGDLGDSGFKLGDLV----------------------CALVQGGGY---- 94 PG E G + A G K GD V CA Q GY Sbjct: 63 PGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSVNG 122 Query: 95 --AEFCAAPLAQCLPVPKGLSALEAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGI 152 AE+ A +P L AA + TV+ + + GE +V G G+ Sbjct: 123 SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGE--WVVISGIGGL 180 Query: 153 GVTAIQIARALGHRVFATAGSADKCRACEELGAERAINYKTEDFEAVVKELTGG 206 G A+Q A+A+G V A DK ++LGA+ ++ + + A V+ + GG Sbjct: 181 GHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGG 234 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.138 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,127,089 Number of extensions: 52430 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 7 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)