BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0264.p (775 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G ... 1390 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 223 4e-59 >gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G [Herbaspirillum seropedicae SmR1] Length = 772 Score = 1390 bits (3598), Expect = 0.0, Method: Compositional matrix adjust. Identities = 731/775 (94%), Positives = 753/775 (97%), Gaps = 3/775 (0%) Query: 1 MVEIEIDGKKVEVQEGSMVMDAANKIGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP 60 MVEIEIDGKKVEVQEGSMVMDAANK+GTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP Sbjct: 1 MVEIEIDGKKVEVQEGSMVMDAANKLGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP 60 Query: 61 ACATPVNNGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS 120 ACATPVN GMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS Sbjct: 61 ACATPVNAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS 120 Query: 121 SRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF 180 SRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF Sbjct: 121 SRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF 180 Query: 181 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK 240 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK Sbjct: 181 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK 240 Query: 241 VMRVLPFENEEVNECWISDKDRFAYEALDSDQRLTKPMLKQDGKWQEVDWQTALEYVAHG 300 VMRVLPFENEEVNECWISDKDRFAYEALDS+QRLTKPMLKQDGKWQEVDWQTALEYVAHG Sbjct: 241 VMRVLPFENEEVNECWISDKDRFAYEALDSEQRLTKPMLKQDGKWQEVDWQTALEYVAHG 300 Query: 301 LRNIRHEHGADSIAALGTPYSTLEELSLLQKLVRGVGSDNVDFRLRQSDFGLDGQIKPWL 360 LRNIRHEHGAD+IAALGTPYSTLEELSLLQK+VRGVGS+N+DFRLRQSDFGLDGQIKPWL Sbjct: 301 LRNIRHEHGADAIAALGTPYSTLEELSLLQKIVRGVGSENIDFRLRQSDFGLDGQIKPWL 360 Query: 361 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMPIANKII 420 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMP+ANKII Sbjct: 361 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMPVANKII 420 Query: 421 AAPSAWLSALAEVAVAVAEAKGVAAPAAYAGVVASDAAKATAASLLSGEGRGVFLGNAAV 480 AAPSAWLSALAEVAVAVAEAKGVAAP+AYAGV ASDAAKATAASLLSG+ +GVFLGNAAV Sbjct: 421 AAPSAWLSALAEVAVAVAEAKGVAAPSAYAGVTASDAAKATAASLLSGDAKGVFLGNAAV 480 Query: 481 QHPQAAQLHAAAQWIAEQTGAKLGVLTDGGNAVGGYLANAVPTPGKGANAAAVFAQPRKA 540 QHPQAAQLHAAAQWIAEQTGAKLGVLT+GGNAVGGYLANAVP AAAVFAQPRKA Sbjct: 481 QHPQAAQLHAAAQWIAEQTGAKLGVLTEGGNAVGGYLANAVPA---AGKAAAVFAQPRKA 537 Query: 541 YLLLNAEPELDSYDPQAARAALGQAEMVVVMSAYQHGADYADVLLPIAPFTETAGTFVNA 600 YLLLNAEPELDSYDPQAA AAL QA+MVVVMSA++HGADYADVLLPIAPFTET+GTFVN Sbjct: 538 YLLLNAEPELDSYDPQAASAALKQADMVVVMSAFKHGADYADVLLPIAPFTETSGTFVNT 597 Query: 601 EGRAQSFNGTVKPAGDARPGWKVLRVLGNLLELENFDYETAESIRDELLGKETDLSARLN 660 EGRAQSFNGTVKPAGDARPGWKVLRVLGNLLELENF+YET+ESIRDELLGKETDLSARLN Sbjct: 598 EGRAQSFNGTVKPAGDARPGWKVLRVLGNLLELENFEYETSESIRDELLGKETDLSARLN 657 Query: 661 NIASVQAQAVQAAAGGVERVADVPIYFADAIARRSPPLLETRDGQAPKAWLSATLAAKLG 720 N+ASVQ +AV AA VERVADVPIYFAD IARRS PLLETRDGQAP+AWLSA LA+KLG Sbjct: 658 NLASVQLRAVSTAAAEVERVADVPIYFADPIARRSAPLLETRDGQAPRAWLSAALASKLG 717 Query: 721 VAEGDKVNVKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA 775 VAEGD+V VKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA Sbjct: 718 VAEGDQVKVKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA 772 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 223 bits (568), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 236/888 (26%), Positives = 369/888 (41%), Gaps = 157/888 (17%) Query: 1 MVEIEIDGKKVEVQEGSMVMDAANKIGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPL- 59 MV +EIDG V V G+ VM A+ + G +P C L +CR+CLVE+EK + + Sbjct: 19 MVTLEIDGVAVTVPAGTSVMRASVEAGINVPKLCATDSLEPFGSCRLCLVEIEKDGRKMK 78 Query: 60 --PA-CATPVNNGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLA--V 114 PA C TP GM V+T + K + ++GVME + +HPLDC C G C+LQD+A V Sbjct: 79 GYPASCTTPCEPGMKVQTQTPKLAEIRRGVMELYISDHPLDCLTCPTNGNCELQDMAGVV 138 Query: 115 GYGGSSSRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEH 174 G Y+ E KD + ++CI C RCVR +E G L + GRG Sbjct: 139 GLREVRYGYEGENHTKMKKDESNPYFTYDPSKCIVCNRCVRACEETQGTFALTINGRGFE 198 Query: 175 SEITT-FVGKTVDSEL--SGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGAN 231 S ++ + ++SE G ++ CP LT K + E S+ + + + VG + Sbjct: 199 SRVSAGQMEAFMESECVSCGACVEACPTATLTEKTVIMLGQA-EHSKVTTCA-YCGVGCS 256 Query: 232 LIVQVKNGKVMRVLPFENEEVNECWISDKDRFAYEALDSDQRLTKPMLKQ--DGKWQEVD 289 ++K +V+R++P+++ + N+ K RFA+ R+ PM+++ + W+EV Sbjct: 257 FKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHKDRILNPMIRKSINDPWREVS 316 Query: 290 WQTALEYVAHGLRNIRHEHGADSIAALGTPYSTLEELSLLQKLVR-GVGSDNVDFRLR-- 346 W+ AL Y A R I+ +HG DSI + + T EE L+QKLVR G++NVD R Sbjct: 317 WEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVC 376 Query: 347 --QSDFGLDGQIKPWLGMSIVE-FSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSV 403 + +GL + G E + + + ++G+ HP+ ++++++ + GAK+ V Sbjct: 377 HSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVFASQIKRRVRQGAKLIV 436 Query: 404 LHATDDDLLMPIANKIIAAP---------------SAWLSALAEVAVA------------ 436 + P +++ +P +A ++ALA V ++ Sbjct: 437 ID--------PRTTEMVKSPHIQADYHLKLRPGTNTAIITALAHVILSEGLQDEAYIQER 488 Query: 437 -------------VAEAKGVAAPAAYAGVVASDAAKATAASLLSGEGRGVFLGNAAVQHP 483 + E A AA GV A + + A +G ++ G +H Sbjct: 489 CDLQSYQDWKEFVLREDNSPEAMAAITGVPA-EIIRGAARLYATGGNAAIYYGLGVTEHA 547 Query: 484 QAAQLHAAAQWIAEQTG--AKLGV----LTDGGNAVGGYLANAVP--TPG-KGANAAAVF 534 Q + +A TG + GV L N G + P PG + + + V Sbjct: 548 QGSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVR 607 Query: 535 AQPRKAY-LLLNAEPELD------------------------SYDP--QAARAALGQAEM 567 Q +A+ + LN EP L DP Q AAL E Sbjct: 608 NQFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMEC 667 Query: 568 VVVMSAY-QHGADYADVLLPIAPFTETAGTFVNAEGRAQSFNGTVKPAGDARPGWKVLRV 626 +VV + A YA V LP + F E GTF NAE R V P + W+V Sbjct: 668 IVVQDLFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVR-KVMPPKAGKSDWEVTVA 726 Query: 627 LGNLLELENFDYETAESIRDELLGKETDLSA----RLNNIASVQAQAVQAAAGGVERVAD 682 L L Y I DE+ +L + S+Q ++A G Sbjct: 727 LAEALGYP-MPYNHPSEIMDEIAALTPSFHGVSYDKLEKLGSIQWPCNESAPEG------ 779 Query: 683 VPI-----------------YFA--DAIARRSPPLL-------------ETRDGQAPKAW 710 PI YFA + + +R P +L +TR + + W Sbjct: 780 TPIMHVGSFIRGKGKFINTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQ-W 838 Query: 711 LSATL-------AAKLGVAEGDKVNVKQGSGSAALTAAIDKKLPENVV 751 S A G+ EGD V V+ +G L A + +K+ VV Sbjct: 839 HSEDRLEIHPHDAEDRGIREGDWVGVESRAGQTVLRATVTEKVQPGVV 886 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,168,670 Number of extensions: 87175 Number of successful extensions: 340 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 333 Number of HSP's successfully gapped: 2 Length of query: 775 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 682 Effective length of database: 1,179,515 Effective search space: 804429230 Effective search space used: 804429230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)