BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0255.p (169 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311110|ref|YP_003775202.1| NTP pyrophosphohydrolase [Herba... 286 6e-79 >gi|300311110|ref|YP_003775202.1| NTP pyrophosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 191 Score = 286 bits (732), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 141/191 (73%), Positives = 155/191 (81%), Gaps = 22/191 (11%) Query: 1 MDAHLKETQKDTRQVYDGHFLKVQKDTIVLPDGKETSREYIKHPGAVXRI---------- 50 MDAHLKETQKD RQVY GHFLKVQ+DT++LPDGK REYIKHPGAV + Sbjct: 1 MDAHLKETQKDNRQVYTGHFLKVQQDTVILPDGKHAVREYIKHPGAVAVLPLFDDGSVLM 60 Query: 51 ------------IPPKAGKIDPGEDTLDCAKRELKEETGYTATEWQYVCTIHNAIAYSDE 98 I AGKIDPGEDTLDCAKREL+EETGYTA +WQYVCTIHNAIAYSDE Sbjct: 61 ERQFRYPLHRVFIEFPAGKIDPGEDTLDCAKRELQEETGYTAAQWQYVCTIHNAIAYSDE 120 Query: 99 HLVLYLARGLKEGERELDEGEFLDIYKAPLAELLEQVRNGQITDVKTVIGVFWLEKIING 158 HLVLY+ARGL +GER+LDEGEFLDIYKAPL EL+EQVRNG+ITDVKTVIGVFWLEKI++G Sbjct: 121 HLVLYVARGLTQGERKLDEGEFLDIYKAPLEELVEQVRNGEITDVKTVIGVFWLEKILSG 180 Query: 159 SWTPPERPAGI 169 +WTPP RPAGI Sbjct: 181 AWTPPPRPAGI 191 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 579,338 Number of extensions: 22788 Number of successful extensions: 47 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 46 Number of HSP's successfully gapped: 1 Length of query: 169 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 90 Effective length of database: 1,245,805 Effective search space: 112122450 Effective search space used: 112122450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 127 (53.5 bits)