BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0250.p (296 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC t... 567 e-163 gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 147 1e-36 gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC t... 147 1e-36 gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC tr... 137 7e-34 gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 112 5e-26 >gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 296 Score = 567 bits (1461), Expect = e-163, Method: Compositional matrix adjust. Identities = 292/296 (98%), Positives = 295/296 (99%) Query: 1 MDILLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNIHGG 60 MDILLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNIHGG Sbjct: 1 MDILLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNIHGG 60 Query: 61 PYLNYWLMIPIVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIFKNVAENIWG 120 PYLNYWLMIPIVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIFKNVAENIWG Sbjct: 61 PYLNYWLMIPIVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIFKNVAENIWG 120 Query: 121 KDDLPFPSPISGTPFQVFGANVQPMQVLVVIGALLIMAAVELFNRKSIYGKAVVATSNDR 180 KDDLPFPSPISG PFQ+FGANVQPMQVLVV+GALLIMAAVELFNRKSIYGKAVVATSNDR Sbjct: 121 KDDLPFPSPISGAPFQIFGANVQPMQVLVVVGALLIMAAVELFNRKSIYGKAVVATSNDR 180 Query: 181 DAAGLMGINTSMVITFSYALSSATAAFAGVLVAPLTLTGATMGAALGLKAFAVAIIGGLT 240 DAAGLMGINTSMVITFSYALSSATAAFAGVLVAPLTLTGATMGAALGLKAFAVAIIGGLT Sbjct: 181 DAAGLMGINTSMVITFSYALSSATAAFAGVLVAPLTLTGATMGAALGLKAFAVAIIGGLT 240 Query: 241 SGMGAIVGGLILGVAETVTGFYISTGYKEVPGLVLLLLVLAVKPAGLFGKTAIKKV 296 SGMGAIVGGLILGVAET+TGFYISTGYKEVPGLVLLLLVLAVKPAGLFGKTAIKKV Sbjct: 241 SGMGAIVGGLILGVAETLTGFYISTGYKEVPGLVLLLLVLAVKPAGLFGKTAIKKV 296 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 147 bits (370), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 97/313 (30%), Positives = 181/313 (57%), Gaps = 23/313 (7%) Query: 1 MDILLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNIH-- 58 MDI +Q + +G+ LG +YA+IA GY + + +NF G+ LM+GA+VGLS++ + Sbjct: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 Query: 59 --GGPYLNYWLM-----IPIVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIF 111 G P + ++ IP+ +V + +E I RP ++ +++ I ++I+ Sbjct: 61 APGLPGIVQLVIAIVGAIPVCIVVS----LLIERIAYRP-LRNAPRLAPLITAIGVSILL 115 Query: 112 KNVAENIWGKDDLPFPSPISGTPFQVFGANVQPMQVLVVIGALLIMAAVELFNRKSIYGK 171 + +A IWG+ LPFP + P + GA + P Q++++ A+L M + L K+ G+ Sbjct: 116 QTLAMMIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGR 175 Query: 172 AVVATSNDRDAAGLMGINTSMVITFSYALSSATAAFAGVL-VAPLTLTGATMGAALGLKA 230 A+ AT+ + AGLMG++ + VI ++A+ + AA AGV+ A + MG GLKA Sbjct: 176 AMRATAENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKA 235 Query: 231 FAVAIIGGLTSGMGAIVGGLILGVAET--------VTGFYISTGYKEVPGLVLLLLVLAV 282 F+ A++GG+ + GA++GG++LG+ E+ +TG ++ + Y+++ ++L++VL + Sbjct: 236 FSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTL 295 Query: 283 KPAGLFGKTAIKK 295 +P+G+ G+ + Sbjct: 296 RPSGIMGERVADR 308 >gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 147 bits (370), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 97/313 (30%), Positives = 181/313 (57%), Gaps = 23/313 (7%) Query: 1 MDILLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNIH-- 58 MDI +Q + +G+ LG +YA+IA GY + + +NF G+ LM+GA+VGLS++ + Sbjct: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 Query: 59 --GGPYLNYWLM-----IPIVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIF 111 G P + ++ IP+ +V + +E I RP ++ +++ I ++I+ Sbjct: 61 APGLPGIVQLVIAIVGAIPVCIVVS----LLIERIAYRP-LRNAPRLAPLITAIGVSILL 115 Query: 112 KNVAENIWGKDDLPFPSPISGTPFQVFGANVQPMQVLVVIGALLIMAAVELFNRKSIYGK 171 + +A IWG+ LPFP + P + GA + P Q++++ A+L M + L K+ G+ Sbjct: 116 QTLAMMIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGR 175 Query: 172 AVVATSNDRDAAGLMGINTSMVITFSYALSSATAAFAGVL-VAPLTLTGATMGAALGLKA 230 A+ AT+ + AGLMG++ + VI ++A+ + AA AGV+ A + MG GLKA Sbjct: 176 AMRATAENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKA 235 Query: 231 FAVAIIGGLTSGMGAIVGGLILGVAET--------VTGFYISTGYKEVPGLVLLLLVLAV 282 F+ A++GG+ + GA++GG++LG+ E+ +TG ++ + Y+++ ++L++VL + Sbjct: 236 FSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTL 295 Query: 283 KPAGLFGKTAIKK 295 +P+G+ G+ + Sbjct: 296 RPSGIMGERVADR 308 >gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 308 Score = 137 bits (346), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 111/305 (36%), Positives = 173/305 (56%), Gaps = 16/305 (5%) Query: 4 LLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNI-----H 58 L Q + +G++LG IYA+IA GY + + G +NF GE M+ + VGL + I + Sbjct: 8 LTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIGVQSGY 67 Query: 59 GGPYLNYWLMIPIVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIFKNVAENI 118 P L +I VLV G L G VE + RP ++ ++S I ++I +N + Sbjct: 68 PLPLLLGGALIVSVLVTG-LYGWTVERVAYRP-LRGGPRLVPLISAIGMSIFLQNYVQIG 125 Query: 119 WGKDDLPFPSPISGT-PFQVFGANVQ---PMQVLVVIG-ALLIMAAVELFNRKSIYGKAV 173 G D+ P ISG FQ+ G++ P +V++G L++M A+ LF +S G+A Sbjct: 126 QGARDMSVPVLISGALEFQM-GSDFTVTIPYSRMVIVGVTLVLMVALTLFIARSRMGRAC 184 Query: 174 VATSNDRDAAGLMGINTSMVITFSYALSSATAAFAGVLVAPLTL--TGATMGAALGLKAF 231 A + D A L+GI+T+ VI+F++ L + AA GVL+A LT+ +G G+KAF Sbjct: 185 RACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIA-LTIGKLNPYIGFIAGIKAF 243 Query: 232 AVAIIGGLTSGMGAIVGGLILGVAETVTGFYISTGYKEVPGLVLLLLVLAVKPAGLFGKT 291 A++GG+ S GA++GG++LG+AET Y+ + YK+V LL+L+L +P GL GK Sbjct: 244 TAAVLGGIGSIPGAMLGGVLLGLAETFAAGYLPSEYKDVVSFGLLVLILLFRPTGLLGKP 303 Query: 292 AIKKV 296 ++KV Sbjct: 304 DVEKV 308 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 112 bits (279), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 85/296 (28%), Positives = 156/296 (52%), Gaps = 7/296 (2%) Query: 3 ILLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNIHGGPY 62 +L Q + + ++LG +YA+ A G+ L F G +N G MLG+ L +V + Sbjct: 1 MLEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMA---- 56 Query: 63 LNYWLMIPIVLVFGALQGMFVEWIGVRPAIKIKS-EFGWIMSTIALAIIFKNVAENIWGK 121 L W+ + + +V L G+ ++ + ++P K + +++TI +AI+ N+++ ++G Sbjct: 57 LPLWVALLLAMVATGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGLFGA 116 Query: 122 DDLPFPS-PISGTPFQVFGANVQPMQVLVVIGALLIMAAVELFNRKSIYGKAVVATSNDR 180 ++ FP I + ++ +QV +++ A ++M + RK+ G+A+ A + Sbjct: 117 ENKRFPQGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESP 176 Query: 181 DAAGLMGINTS-MVITFSYALSSATAAFAGVLVAPLTLTGATMGAALGLKAFAVAIIGGL 239 AA L+GIN + + S+A ++ A ++ MG + K AV I+GG+ Sbjct: 177 KAAYLLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILGGM 236 Query: 240 TSGMGAIVGGLILGVAETVTGFYISTGYKEVPGLVLLLLVLAVKPAGLFGKTAIKK 295 GA++GGL LG AE +T YIS+ +++ LL L+L VKP+G+FGK +K Sbjct: 237 GDIRGAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGKVLERK 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.142 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 885,644 Number of extensions: 39677 Number of successful extensions: 341 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 319 Number of HSP's successfully gapped: 25 Length of query: 296 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 211 Effective length of database: 1,217,395 Effective search space: 256870345 Effective search space used: 256870345 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)