BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0241.p (311 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313481|ref|YP_003777573.1| isoleucine patch superfamily ac... 144 1e-35 >gi|300313481|ref|YP_003777573.1| isoleucine patch superfamily acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 188 Score = 144 bits (363), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 79/154 (51%), Positives = 101/154 (65%), Gaps = 2/154 (1%) Query: 1 MSFYVHPNALC-ESAHIGENTRIWAFAHILPQAVIGSDCNICDGVFVENDVVVGDRVTIK 59 M+++ H AL E A IGE TRIW +AHI A IG C+ VFV NDV++G+ V ++ Sbjct: 1 MTYHAHETALVDEGAQIGEATRIWHWAHICSGARIGERCSFGQNVFVGNDVLIGNNVKVQ 60 Query: 60 CGVQLWDGVHLENDVFIGPNVTFTNDLRPRSK-QYPASFAKTLVCEGASIGANATILPGL 118 V ++D V LE+DVF GP++ FTN PRS + +TLV GASIGANATI+ G Sbjct: 61 NNVSIYDAVTLEDDVFCGPSMVFTNVNNPRSAVSRKHEYRRTLVRRGASIGANATIVCGH 120 Query: 119 VIGRSAMIGAGAVVTQSVPPYAIVVGNPARITGY 152 IG A IGAGAVVT+ VP YA++VG PAR G+ Sbjct: 121 EIGEYAFIGAGAVVTRDVPAYALMVGTPARRIGW 154 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,003,055 Number of extensions: 42124 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 1 Length of query: 311 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 226 Effective length of database: 1,217,395 Effective search space: 275131270 Effective search space used: 275131270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)