BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0232.p (182 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 343 6e-96 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 107 8e-25 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 96 2e-21 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 96 2e-21 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 94 4e-21 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 343 bits (879), Expect = 6e-96, Method: Compositional matrix adjust. Identities = 169/182 (92%), Positives = 176/182 (96%) Query: 1 VLRLFTTTDQELGRVTALVNNAGILDHQSRLDAMSAERITRVLVANVTGSLLCAREAVKR 60 VL+LF T D ELGRVTALVNNAG+LDHQSRL+ MSAERI RVL+ANVTGSLLCAREAVKR Sbjct: 70 VLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLCAREAVKR 129 Query: 61 MSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAVR 120 MSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAVR Sbjct: 130 MSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAVR 189 Query: 121 PGLIYTDIHASGGEAGRVDRLKDAVPMKRGGTAEEVANAILWLLSDESSYATGTFIDVSG 180 PGLIYTDIHASGGEAGRVDRLKDAVPMKRGG+A EVANAILWLLS+E+SYATGTFIDVSG Sbjct: 190 PGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATGTFIDVSG 249 Query: 181 GR 182 GR Sbjct: 250 GR 251 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 107 bits (266), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 64/174 (36%), Positives = 101/174 (58%), Gaps = 6/174 (3%) Query: 11 ELGRVTALVNNAGILDHQSRLDAMSAERITRVLVANVTGSLLCAREAVKRMSTRHGGQGG 70 ++GR+ L+NNAG L ++ L+ ++ E ++ N + R + M + GG Sbjct: 81 QMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAFTRAVIPVMRAQGGGN-- 138 Query: 71 AIVNLSSMAAKLGG-PGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAVRPGLIYTDIH 129 I+N++S+AA+ GG PG + YAASKG + T G+AKE+ A+ IRVNAV PG+I T Sbjct: 139 -IINVTSVAARHGGGPGALI-YAASKGFVSTLTRGMAKELLADKIRVNAVAPGVIMTPFQ 196 Query: 130 ASGGEAGRVDRLKDAVPMKRGGTAEEVANAILWLLSDE-SSYATGTFIDVSGGR 182 +++ + +PM R G +E + A L+L S++ S Y TG IDV+GG+ Sbjct: 197 ERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQIIDVNGGQ 250 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 95.9 bits (237), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 65/177 (36%), Positives = 94/177 (53%), Gaps = 5/177 (2%) Query: 5 FTTTDQELGRVTALVNNAGILDHQSRLDAMSAERITRVLVANVTGSLLCAREAVKRMSTR 64 F +++ GR LVNNAG+ S LD E + + NVTG LL + A + M + Sbjct: 76 FAHIEKDYGRCDVLVNNAGVARTYSFLD-YPLENWLQTMNVNVTGVLLAGQHAARLMVKQ 134 Query: 65 HGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAVRPGLI 124 Q G IVN+SS++ G G Y SK A+ T +A E+A GI VN+V PG + Sbjct: 135 ---QWGRIVNISSISGIRAGAGR-TAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPV 190 Query: 125 YTDIHASGGEAGRVDRLKDAVPMKRGGTAEEVANAILWLLSDESSYATGTFIDVSGG 181 T + + + VPM+R GT +E+A+A+ +L ++ES+Y TG I V GG Sbjct: 191 DTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 95.5 bits (236), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 64/179 (35%), Positives = 99/179 (55%), Gaps = 10/179 (5%) Query: 5 FTTTDQELGRVTALVNNAGIL-DHQSRLDAMSAERITRVLVANVTGSLLCAREAVKRMST 63 F E+G V LVNNAGI D Q R MS + V+ N+ ++ ++ M Sbjct: 72 FEKVRAEIGEVDVLVNNAGITRDGQFR--KMSKDDWDAVIDTNLNSLFNVTKQVIEGMCE 129 Query: 64 RHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAVRPGL 123 R G+ I+N+SS+ + G G+ +Y+ +K I FT+ LA+EVA +G+ VN V PG Sbjct: 130 RGFGR---IINISSVNGQKGQFGQ-TNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGY 185 Query: 124 IYTD-IHASGGEAGRVDRLKDAVPMKRGGTAEEVANAILWLLSDESSYATGTFIDVSGG 181 I TD + A E ++++ +P+KR G EE+A+ + W+ S E YATG+ ++GG Sbjct: 186 IGTDMVRAIRPEV--LEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGG 242 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 94.4 bits (233), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 61/177 (34%), Positives = 98/177 (55%), Gaps = 13/177 (7%) Query: 10 QELGRVTALVNNAGILDHQSRLDAMSAERITRVLVANVTGSLLCAREAVKRMSTRHGGQG 69 Q+ GR+ LVNNAGI L+ A+ R+L ++ G+L ++ + M + Q Sbjct: 77 QQFGRIDILVNNAGITQPVKTLEITGADY-DRILDVSLRGTLYMSQAVLPAMRAQ---QN 132 Query: 70 GAIVNLSSMAAK-----LGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAVRPGLI 124 G+I+ +SS++A+ LGGP Y+A+K + +A+E EGIRVN++ PGLI Sbjct: 133 GSIICISSVSAQRGGGILGGP----HYSAAKAGVLGLARAMAREFGPEGIRVNSITPGLI 188 Query: 125 YTDIHASGGEAGRVDRLKDAVPMKRGGTAEEVANAILWLLSDESSYATGTFIDVSGG 181 TDI + + + +P+ R G A+++ A ++L SD S Y TG +DV+GG Sbjct: 189 GTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGITLDVNGG 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 590,855 Number of extensions: 24130 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 38 Length of query: 182 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 102 Effective length of database: 1,241,070 Effective search space: 126589140 Effective search space used: 126589140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)