BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0224.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 785 0.0 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 179 3e-46 gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 110 3e-25 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 785 bits (2027), Expect = 0.0, Method: Compositional matrix adjust. Identities = 405/457 (88%), Positives = 434/457 (94%) Query: 1 MTLSLKPGTGHGISSRGKGFMRRVAPRLDRRLGELQAMPRPRLAVLACTLLLALLVLWSM 60 MTLSLKP GI +RG+GFMRR+APRLDRRLGEL MPRPRLAVLA T+LLALLVLWS+ Sbjct: 1 MTLSLKPNATPGIGARGQGFMRRLAPRLDRRLGELATMPRPRLAVLAVTVLLALLVLWSV 60 Query: 61 LAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAVVRKGQTLISIADVRANADL 120 APIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGA V+KGQTLISI+DVRANADL Sbjct: 61 FAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRANADL 120 Query: 121 GEGRVKILGLRAKIARLSAEAAGAASLQMPADLDHDDPAVQSEQAAFAARSAKLMQEMSV 180 GEGRVKILGLRAKIARLSAEA+GA+SLQMP DL+ DPAVQSEQAAFAARS+KLMQEMSV Sbjct: 121 GEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQEMSV 180 Query: 181 LREQAAQRQAELGEAVSRQKSQASELDLAQRQYKLIHNMLERNAASQLEDIQAAARVQDA 240 LREQ+AQRQAELGEA+SRQKSQASEL+LAQRQYKLIH+ML RNAASQLEDIQAA+RVQDA Sbjct: 181 LREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAASRVQDA 240 Query: 241 QSKLSANDAMIPRLKAAIAESQAKMGEASSRFRSDARAELTAAETELARLQEEIKSRNDR 300 QSKL+A +AMIPRL AAIAE+QAK GEA+SRFRSDAR ELTAAETELARLQEEIKSRNDR Sbjct: 241 QSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKSRNDR 300 Query: 301 VSRSEVKAPMDGVVNRVMINTVGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELATGA 360 VSRSEVKAPMDGVVNRVMINT+GGVVRPGDPIIEMTPSDDKLVIEARISPTDRAEL TGA Sbjct: 301 VSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELVTGA 360 Query: 361 KARVKVSAYDYGVYGAMEGVVTEISADTVPDENNPRGERSYRIKIAVQRGDNDLQRKLAL 420 +ARVKVSAYDYGVYGAM+GVVTEISADTVPDE +PRGER YR+KI V+RG ++LQ+KLAL Sbjct: 361 RARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDELQQKLAL 420 Query: 421 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR 457 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR Sbjct: 421 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR 457 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 179 bits (455), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 119/408 (29%), Positives = 207/408 (50%), Gaps = 54/408 (13%) Query: 54 LLVLWSMLAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAVVRKGQTLISIAD 113 +L+LW+ ++ ID V R G+++A Q +Q +GG V ILV+EG V++GQ L+ + Sbjct: 26 VLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEGEAVKQGQLLMVLEQ 85 Query: 114 VRANADLGEGRVKILGLRAKIARLSAEAAGAASLQMPADLDHDDPAVQSEQAAFAARSAK 173 R L + R K+ LR +ARL AE G P LD+ + + +++ + R Sbjct: 86 DRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNFEPDLLDYKE-YIANQKDLYVKRKIL 144 Query: 174 LMQEMSVLREQAAQRQAELGEAVSRQKSQASELDLAQRQYKLIHNMLERNAASQLEDIQA 233 + Q+++ L++ L LA ++ ++ + LER Sbjct: 145 IDQDLASLQDM---------------------LGLANQELEM-NKSLERTG--------- 173 Query: 234 AARVQDAQSKLSANDAMIPRLKAAIAESQAKMGEASSRFRSDARAELTAAETELARLQEE 293 A + RL+ +A+ +A++ +++ +A ++T + +L +E+ Sbjct: 174 -----------DVGRADVLRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQ 222 Query: 294 IKSRNDRVSRSEVKAPMDGVVNRVMINTVGGVVRPGDPIIEMTPSDDKLVIEARISPTDR 353 ++ R + +E+KAP DGVV + + T+GGVVR GD ++E+ P D+ L++EA++S D Sbjct: 223 LEDRTQVLEHTELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDN-LIVEAKVSTADS 281 Query: 354 AELATGAKARVKVSAYDYGVYGAMEGVVTEISADTVPDENNPRGERSYRIKIAVQ----- 408 A + G A VK+ AYDY +YG + G V +S DT+ DE YR+ IA++ Sbjct: 282 AYVKVGQAANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEEAVFK 341 Query: 409 -RGDNDLQRKLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 R + +Q K PGMTA+ +I RTV +L P+++ Q ++ E Sbjct: 342 GRKADAIQVK----PGMTATVEIKANTRTVLSFLTKPIAKTLQESMGE 385 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 110 bits (275), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 128/459 (27%), Positives = 212/459 (46%), Gaps = 47/459 (10%) Query: 10 GHGISSRGK----GFMRRVAPRLDRRLGELQAMPRPRLAVLACTLLLALLV---LWSMLA 62 GH RG F + A L L LQ P R LL+ +++ LWS+L Sbjct: 22 GHAWRQRGHYRSDFFNKDEAEFLPAALS-LQEAPDSRSLRWTARLLVGMVIFALLWSLLG 80 Query: 63 PIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAVVRKGQTLISIADVRANADLGE 122 ID+VV +G+++ S + I ++ V AILV EG +V+ G+ LI + ++A+ + Sbjct: 81 RIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLVKAGEVLIELDSSSSDAERDK 140 Query: 123 GRVKILGLRAKIARLSAEAAGAASLQMP--ADLDHDDPAVQSEQAAFAARSAKLMQEMSV 180 + R + AR A +L+ P A + P V QA + A ++L+ + Sbjct: 141 AADAVAQARLQAARAQAMMEAIQNLRTPELAQV----PGV--AQAQWEAVRSQLVAQYQD 194 Query: 181 LREQAAQRQAELGEAVSRQKSQASELDLAQRQYKLIHNMLERNAASQ---LEDIQAAARV 237 R R L + +SR + L LA ++ ++ + S+ LE QA RV Sbjct: 195 FRA----RLGRLDDEISRY---GAALKLATQRASDYQTLVADHTVSRHAWLEKEQA--RV 245 Query: 238 QDAQSKLSANDAMIPRLKAAIAESQAKMGEASSRFRSDARAELTAAETELARLQEEIKSR 297 D Q +L +DA R + A +Q + SR +D E + Q++ + Sbjct: 246 -DLQGQL--DDA---RNQRATLVAQTRKDAHDSRIEADKTIEAS---------QQDQRRA 290 Query: 298 NDRVSRSEVKAPMDGVVNRVMINTVGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELA 357 ++ ++ AP+ G V ++ ++T+GGVV P++++ P D ++ +EA + D + Sbjct: 291 DEHSKLLKLVAPVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVE 350 Query: 358 TGAKARVKVSAYDYGVYGAMEGVVTEISADTVPDENNPRGERSYRIKIAVQRGDNDLQ-R 416 G A VK+ A+DY YG + V IS D + DE Y +I + + + R Sbjct: 351 IGQAAEVKIDAFDYTKYGTLPARVVHISRDAIQDEKKG---LLYSARIVLDQSSLIVDGR 407 Query: 417 KLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 K+ L GM A+ +I G R V +Y +SPL R + AL E Sbjct: 408 KVQLSAGMAANVEIKTGNRRVIEYALSPLIRHQKEALHE 446 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,165,825 Number of extensions: 41575 Number of successful extensions: 256 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 5 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)