BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0219.p (614 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312840|ref|YP_003776932.1| methyl-accepting chemotaxis I p... 699 0.0 gi|300311131|ref|YP_003775223.1| hypothetical protein Hsero_1805... 313 4e-86 gi|300310582|ref|YP_003774674.1| methyl-accepting chemotaxis I p... 175 1e-44 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 126 6e-30 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 125 1e-29 >gi|300312840|ref|YP_003776932.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 646 Score = 699 bits (1803), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/452 (78%), Positives = 409/452 (90%) Query: 1 MRSWINHLNYSRKFVLIGALAFMLFIVPTTLLVRSNLDDLATAQRESLALAPGRELLLLL 60 MRS INHL +SRKF LIGALAF+LFIVPTTLLVRSNL DLA A+RE LA+ PGR+LLL+L Sbjct: 1 MRSLINHLRFSRKFALIGALAFLLFIVPTTLLVRSNLQDLAIAEREQLAVEPGRDLLLML 60 Query: 61 KQMQQHRGLSALYLADGDGAAASRAERQKEVDATFDRVRSGLKPLEDKALTDALDGLNAD 120 KQMQQHRGLSALYL+D + AA RAERQK+V+AT +RVR+G+ PL+DK L ++L+ + + Sbjct: 61 KQMQQHRGLSALYLSDTNAGAADRAERQKQVEATLERVRTGILPLQDKELMESLERVASQ 120 Query: 121 WRALTQSIASKSINGAQSNVQHAQLIKRELDLLDDVANSSGLALEAEAALFYVQRAVMSE 180 WRAL Q+++SK+I GAQSN+QHA+LI ELDL+D +ANSSGLALEAEAALFYVQRAVM+E Sbjct: 121 WRALAQAVSSKAITGAQSNLQHAELIALELDLIDQLANSSGLALEAEAALFYVQRAVMNE 180 Query: 181 LPQLTEALGQARARGAVMLGRGQADAEERARLENLSDRARRSYESARRSLTLAAGRHPLP 240 LPQLTEALGQ RARGAVMLGRGQA AEERARLENL+DRARRSY+SAR+SLTLAAGRH LP Sbjct: 181 LPQLTEALGQTRARGAVMLGRGQASAEERARLENLADRARRSYDSARKSLTLAAGRHALP 240 Query: 241 AEVESARAAAVTAADEAFKMADSAIVRADQLTMPAPQWVSTMTRIIDTQFVLVTTSFDMM 300 A +E ARA A+ AA+E FK+ADSAI+RAD L+MPAP+WV+TMTRIIDTQFVLV SFD+M Sbjct: 241 ANLERARAEALKAAEEGFKLADSAIIRADNLSMPAPEWVATMTRIIDTQFVLVNASFDLM 300 Query: 301 RDELQAKIPRLRNELIVLCASLAVLAMAALWLMVAITRATTSSLSEAVRLTEAVSAGDLT 360 RDELQAKIPR+RNEL++LCA L +LA AALW+MVAITRATTS++++AVRLTEA+SAGDLT Sbjct: 301 RDELQAKIPRVRNELLMLCALLGLLAAAALWVMVAITRATTSAINDAVRLTEAISAGDLT 360 Query: 361 QHVEVKGSDEVARLLMAMQAMTAQLSMVVGSVRSNSESVATASAQIAQGNADLSKRTEEQ 420 QHV++ GSDEVARLL AMQAMTA+LS VV SVRSNSESVATASAQIAQGNADLSKRTEEQ Sbjct: 361 QHVQINGSDEVARLLTAMQAMTARLSTVVSSVRSNSESVATASAQIAQGNADLSKRTEEQ 420 Query: 421 ASALQQTAASMEELGSTVQQNADGARQAGQLA 452 ASALQQTAASMEELGSTV QNADGARQAG LA Sbjct: 421 ASALQQTAASMEELGSTVAQNADGARQAGDLA 452 >gi|300311131|ref|YP_003775223.1| hypothetical protein Hsero_1805 [Herbaspirillum seropedicae SmR1] Length = 553 Score = 313 bits (801), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 157/165 (95%), Positives = 160/165 (96%), Gaps = 1/165 (0%) Query: 447 QAGQLALSTALNSLQAQQGEISNMNEKNAQNIAQLPALLNQISVRLADEMGAIQYQGELQ 506 QAGQLALS+AL SLQ QQGEI+NMNEKNAQNIAQLPALLNQISVRLADEMGAIQYQGELQ Sbjct: 389 QAGQLALSSALTSLQTQQGEIANMNEKNAQNIAQLPALLNQISVRLADEMGAIQYQGELQ 448 Query: 507 ATVAARLNELGNTIGENSSNMARDALSARQLQIDLAKHMAMQNDRVKNLGAIPQSLTSIT 566 ATVAARLNELGNTIGENSSNMARDALSARQLQIDLAKHMA QNDRVKNLGAIPQSLTSIT Sbjct: 449 ATVAARLNELGNTIGENSSNMARDALSARQLQIDLAKHMATQNDRVKNLGAIPQSLTSIT 508 Query: 567 EALMRQNINWQTVIDEMRTGRQTMVKDLRLELREAIREITATNRG 611 EALMRQNINWQTVIDEMRTGRQTMVKDLRLE+REAIREITA RG Sbjct: 509 EALMRQNINWQTVIDEMRTGRQTMVKDLRLEMREAIREITAV-RG 552 >gi|300310582|ref|YP_003774674.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 668 Score = 175 bits (443), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 155/457 (33%), Positives = 237/457 (51%), Gaps = 3/457 (0%) Query: 1 MRSWINHLNYSRKFVLIGALAFMLFIVPTTLLVRSNLDDLATAQRESLALAPGRELLLLL 60 M + +L +KF L+G L +LF VPTTL S + Q+E + P R LL + Sbjct: 1 MGQLLANLKLWQKFALLGLLGLVLFGVPTTLYFNSTERVIRFKQQEIAGVEPVRRLLRSV 60 Query: 61 KQMQQHRGLSALYLADGDGAAASRAERQKEVDATFDRVRSGLKPL--EDKALTDALDGLN 118 + MQQHRG+SA+ L A R + EVD + L+ + +D + Sbjct: 61 QLMQQHRGMSAVMLNGAADMAGPRQAKAAEVDQALAALAGALEEVGRDDTRIPVLFKNQL 120 Query: 119 ADWRALTQSIASKSINGAQSNVQHAQLIKRELDLLDDVANSSGLALEAEAALFYVQRAVM 178 A W + ++AS+SI +S H+ I DL + + + ++ + + A Sbjct: 121 AAWTKVRDAVASRSITPPESFAAHSAAIAALFDLNERLLDYYHYTIDPDFDSTQLINAAF 180 Query: 179 SELPQLTEALGQARARGAVMLGRGQADAEERARLENLSDRARRSYESARRSLTLA-AGRH 237 +P LTE LG+ARARGA +L + + EER L + +RA+ SAR+S A Sbjct: 181 IAMPALTEDLGRARARGASLLLKKELKLEERLELVAMLERAQERLSSARKSFAKGVAANA 240 Query: 238 PLPAEVESARAAAVTAADEAFKMADSAIVRADQLTMPAPQWVSTMTRIIDTQFVLVTTSF 297 + ++ S A +E K+ ++ +L+ P + T ID QF + Sbjct: 241 EIGVKLGSTFEKADRLGNEVMKLTREQVLLPQELSFPPDAYFKQFTLAIDEQFKAIGEVT 300 Query: 298 DMMRDELQAKIPRLRNELIVLCASLAVLAMAALWLMVAITRATTSSLSEAVRLTEAVSAG 357 +++ +L + LR E + + ++ LA+ WL V ITR+ T + +V + V+ Sbjct: 301 EVLAGQLDTQNAALRREQLTVLGAIFALALLVAWLGVMITRSVTQPILASVAMAGRVADY 360 Query: 358 DLTQHVEVKGSDEVARLLMAMQAMTAQLSMVVGSVRSNSESVATASAQIAQGNADLSKRT 417 DLT +G DE A+LL ++ AMTA L +VG VR++ E + AS +IA GNADLS+RT Sbjct: 361 DLTARSVAQGRDESAQLLGSLNAMTASLGGIVGRVRTSIEVIQHASREIATGNADLSRRT 420 Query: 418 EEQASALQQTAASMEELGSTVQQNADGARQAGQLALS 454 E QAS+L++TA++MEEL STVQ NA+ ARQA QLA S Sbjct: 421 ESQASSLEETASAMEELTSTVQNNAENARQADQLAGS 457 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 126 bits (316), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 95/248 (38%), Positives = 146/248 (58%), Gaps = 29/248 (11%) Query: 264 AIVRADQLTMPAPQWVSTMTRIIDTQ--FVLVTTSF-DMMRDELQAKIPR------LRNE 314 A+V+A T A ++V + +I DTQ ++ + +F D +LQA+ + + E Sbjct: 137 ALVKAGNST-SADEYV--LRKITDTQNNYLALARAFADSQDAQLQAEGAKAVADGAMAIE 193 Query: 315 LIVLCASLAVLAMAALWLMVAITRATTSSLSEAVRLTEAVSAGDLTQHVEVKGSDEVARL 374 + + C+ AVLA L ++A R+ L+EAVR+ E V+AGDLT +E DE +L Sbjct: 194 VTLACSMAAVLAAIILGYLMA--RSIVKPLTEAVRVAENVAAGDLTTRIEPHSRDETGQL 251 Query: 375 LMAMQAMTAQLSMVVGSVRSNSESVATASAQIAQGNADLSKRTEEQASALQQTAASMEEL 434 + A++ M L +V VR +ES+ TAS +IA GN DLS RTE+QA +L++TA++MEE+ Sbjct: 252 MQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLEETASAMEEM 311 Query: 435 GSTVQQNADGARQAGQLALSTALNSLQAQQ--GEISN-MNEKNAQNIAQLPALLNQISVR 491 STV+QNAD ARQA Q+A+S + +LQ Q G + + M+E +NQ S + Sbjct: 312 TSTVRQNADNARQANQMAVSASEIALQGGQIVGRVVHTMDE------------INQSSRK 359 Query: 492 LADEMGAI 499 + D +G I Sbjct: 360 IVDIIGVI 367 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 125 bits (313), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 79/185 (42%), Positives = 114/185 (61%), Gaps = 12/185 (6%) Query: 315 LIVLCASLAVLAMAALWLMVAITRATTSSLSEAVRLTEAVSAGDLTQHVEVKGSDEVARL 374 +IV A+ LAM L V ++R L EAV + + V+ GDLT +E G DE +L Sbjct: 193 IIVTVAACIGLAM---LLAVWVSRLIARPLEEAVSVAQRVAGGDLTTRIEPAGKDETGQL 249 Query: 375 LMAMQAMTAQLSMVVGSVRSNSESVATASAQIAQGNADLSKRTEEQASALQQTAASMEEL 434 L+A+++M L +VG VR ++++ TAS +IA GN DLS RTEEQAS+L++TA++MEEL Sbjct: 250 LVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQASSLEETASAMEEL 309 Query: 435 GSTVQQNADGARQAGQLALSTALNSLQAQQGEISNMNEKNAQNIAQLPALLNQISVRLAD 494 STV+QNAD ARQA QLA+S + + +Q G + N + +N S ++AD Sbjct: 310 TSTVKQNADNARQANQLAVSA--SEVASQGGAVVN-------QVVDTMGSINASSRKIAD 360 Query: 495 EMGAI 499 +G I Sbjct: 361 IIGVI 365 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.125 0.330 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,355,721 Number of extensions: 43174 Number of successful extensions: 508 Number of sequences better than 1.0e-08: 65 Number of HSP's gapped: 455 Number of HSP's successfully gapped: 65 Length of query: 614 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 523 Effective length of database: 1,188,985 Effective search space: 621839155 Effective search space used: 621839155 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)