BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0216.p (154 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311133|ref|YP_003775225.1| hemolysin-adenylate cyclase [He... 103 4e-24 >gi|300311133|ref|YP_003775225.1| hemolysin-adenylate cyclase [Herbaspirillum seropedicae SmR1] Length = 2656 Score = 103 bits (258), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 55/81 (67%), Positives = 65/81 (80%), Gaps = 1/81 (1%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 VTGGSA+DTFIA+AAAN FDG GG+HNRVSYA+S+V ETV+L G G+GG GSLA GDTY Sbjct: 1720 VTGGSANDTFIASAAANRFDGGGGSHNRVSYASSTVSETVDLFNGNGTGGAGSLANGDTY 1779 Query: 61 TNIQD-XGSVPPTISMAAAAA 80 TNIQD G + +A+AAA Sbjct: 1780 TNIQDVTGGTADDVFIASAAA 1800 Score = 103 bits (257), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 55/80 (68%), Positives = 65/80 (81%), Gaps = 2/80 (2%) Query: 2 TGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTYT 61 TGGSA+DTF+A+AAAN FDG GG+HNRVSYANS+VDET+NL++ G+GG GSLA GDTY Sbjct: 1913 TGGSANDTFVASAAANAFDGGGGSHNRVSYANSTVDETINLVSNTGTGGAGSLANGDTYA 1972 Query: 62 NIQD--XGSVPPTISMAAAA 79 NIQD GS TI +AAA Sbjct: 1973 NIQDVTGGSGNDTIVASAAA 1992 Score = 102 bits (254), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 55/100 (55%), Positives = 66/100 (66%), Gaps = 1/100 (1%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 VTGGS +DTF N AANNFDG GGTHNRV+Y++S+V ET++L+ GQGSGG GSLA GD Y Sbjct: 1524 VTGGSGNDTFYGNTAANNFDGGGGTHNRVNYSSSTVAETIDLLNGQGSGGAGSLANGDRY 1583 Query: 61 TNIQDX-GSVPPTISMAAAAATTGSATAPPPSRRRSTCSP 99 TNIQD G + +A+AAA R S S Sbjct: 1584 TNIQDATGGSADDVFIASAAANRFDGGGGSLHNRVSYASS 1623 Score = 101 bits (252), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 55/81 (67%), Positives = 59/81 (72%), Gaps = 2/81 (2%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 VTGG +DTF N AAN FDG GGTHNRVSYA S+V ETV+L GQGSGG GSLA GDTY Sbjct: 1847 VTGGGGNDTFYGNTAANTFDGGGGTHNRVSYATSTVAETVDLFLGQGSGGAGSLANGDTY 1906 Query: 61 TNIQDX--GSVPPTISMAAAA 79 TNIQD GS T +AAA Sbjct: 1907 TNIQDATGGSANDTFVASAAA 1927 Score = 97.8 bits (242), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 49/65 (75%), Positives = 56/65 (86%), Gaps = 3/65 (4%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 VTGG+ADD FIA+AAAN FDG GGTHNRVSYANS+ ET+NL++GQGS G+LAAGDTY Sbjct: 1785 VTGGTADDVFIASAAANAFDGGGGTHNRVSYANSNAAETINLVSGQGS---GTLAAGDTY 1841 Query: 61 TNIQD 65 NIQD Sbjct: 1842 LNIQD 1846 Score = 91.3 bits (225), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 47/65 (72%), Positives = 53/65 (81%), Gaps = 1/65 (1%) Query: 2 TGGSADDTFIANAAANNFDGSGGT-HNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 TGGSADD FIA+AAAN FDG GG+ HNRVSYA+S+ DET++L G GSGG SLA GDTY Sbjct: 1590 TGGSADDVFIASAAANRFDGGGGSLHNRVSYASSTADETIDLYNGTGSGGLNSLANGDTY 1649 Query: 61 TNIQD 65 NIQD Sbjct: 1650 ANIQD 1654 Score = 90.5 bits (223), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 53/65 (81%), Positives = 59/65 (90%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 VTGGSADD F+A+AAAN FDG GG+HNRVSYANS+VDETVNL++GQGSGG GSLA GDTY Sbjct: 1328 VTGGSADDLFVASAAANAFDGGGGSHNRVSYANSTVDETVNLVSGQGSGGIGSLANGDTY 1387 Query: 61 TNIQD 65 NIQD Sbjct: 1388 ANIQD 1392 Score = 90.1 bits (222), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 48/80 (60%), Positives = 57/80 (71%), Gaps = 1/80 (1%) Query: 2 TGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTYT 61 TGGS +DTF N AAN FDG GG+HNRV+Y+ S+V ETV+L GSGG GSLA GDTY Sbjct: 1394 TGGSGNDTFYGNTAANRFDGGGGSHNRVNYSGSTVAETVDLFNNVGSGGAGSLANGDTYA 1453 Query: 62 NIQD-XGSVPPTISMAAAAA 80 NIQD G + +A+AAA Sbjct: 1454 NIQDVTGGTADDVFIASAAA 1473 Score = 84.7 bits (208), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 55/66 (83%), Positives = 59/66 (89%), Gaps = 1/66 (1%) Query: 1 VTGGSADDTFIANAAANNFDGSGGT-HNRVSYANSSVDETVNLITGQGSGGTGSLAAGDT 59 VTGG+ADD FIA+AAAN FDG GG+ HNRVSYANSS DETVNL+TGQGSGGTGSLA GDT Sbjct: 1458 VTGGTADDVFIASAAANAFDGGGGSLHNRVSYANSSADETVNLVTGQGSGGTGSLANGDT 1517 Query: 60 YTNIQD 65 Y NIQD Sbjct: 1518 YINIQD 1523 Score = 84.7 bits (208), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 55/81 (67%), Positives = 61/81 (75%), Gaps = 2/81 (2%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 VTGG+ +DTF+A AAN FDG GGTHNRVSYANS+V ETV+LI G GSGG GS AAGD Y Sbjct: 1655 VTGGAGNDTFVATVAANTFDGGGGTHNRVSYANSTVAETVDLIAGLGSGGAGSFAAGDVY 1714 Query: 61 TNIQD--XGSVPPTISMAAAA 79 TNIQD GS T +AAA Sbjct: 1715 TNIQDVTGGSANDTFIASAAA 1735 Score = 75.1 bits (183), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 54/82 (65%), Positives = 60/82 (73%), Gaps = 2/82 (2%) Query: 1 VTGGSADDTFIANAAANNFDGSGGT-HNRVSYANSSVDETVNLITGQGSGGTGSLAAGDT 59 VTGGSADD IANAAANN DG GG+ HNRVSY NS+V ETV+L GSGG GSLA GDT Sbjct: 1262 VTGGSADDLIIANAAANNIDGGGGSLHNRVSYQNSTVSETVDLFNNVGSGGVGSLANGDT 1321 Query: 60 YTNIQD-XGSVPPTISMAAAAA 80 Y NIQD G + +A+AAA Sbjct: 1322 YANIQDVTGGSADDLFVASAAA 1343 Score = 74.7 bits (182), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 40/66 (60%), Positives = 48/66 (72%), Gaps = 4/66 (6%) Query: 1 VTGGSADDTFIANAAANNFDG-SGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDT 59 +TG DDTF+A+ AANNFDG +G HNRVSYA+SS ET++L G GTG+ A GD Sbjct: 1199 LTGSQFDDTFVASLAANNFDGGTGSLHNRVSYAHSSASETIDLFNGS---GTGTYAQGDR 1255 Query: 60 YTNIQD 65 YTNIQD Sbjct: 1256 YTNIQD 1261 Score = 61.2 bits (147), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 45/98 (45%), Positives = 59/98 (60%), Gaps = 7/98 (7%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 V G + DDTF+A+ N FDG G+ N VSYANS+ TVNL+TG G+GG A GDT+ Sbjct: 1070 VIGSAYDDTFVADNNHNVFDGRQGS-NTVSYANSTAAVTVNLVTGTGTGG---FADGDTF 1125 Query: 61 TNIQD-XGSVPPTISMAAAAATT--GSATAPPPSRRRS 95 TNIQ+ GS + +A++ A T G +A R S Sbjct: 1126 TNIQNVVGSAQDDLIIASSDANTIDGGLSATGAHNRVS 1163 Score = 59.7 bits (143), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 33/65 (50%), Positives = 45/65 (69%), Gaps = 3/65 (4%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 V G + DDTF+ NA AN+FDG G ++ VSYA S+ +VN I+G+G+GG A GDTY Sbjct: 630 VIGTAGDDTFVGNADANSFDGGSGGNDTVSYAGSAQGVSVNFISGRGTGGN---AEGDTY 686 Query: 61 TNIQD 65 +IQ+ Sbjct: 687 NHIQN 691 Score = 58.2 bits (139), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 35/65 (53%), Positives = 46/65 (70%), Gaps = 4/65 (6%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 +TGG+ +DTF+A+ A NNFDG G+ NRVSYA S+ TV+L+ G G+GG A DTY Sbjct: 946 LTGGAGNDTFVASLAENNFDGGAGS-NRVSYAASTSAVTVDLVLGVGTGG---YANNDTY 1001 Query: 61 TNIQD 65 NIQ+ Sbjct: 1002 ANIQN 1006 Score = 56.2 bits (134), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 33/60 (55%), Positives = 41/60 (68%), Gaps = 1/60 (1%) Query: 7 DDTFIANAAANNFDG-SGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTYTNIQD 65 DDTF+A+A AN+FDG +G HNRVSYA S+ TV+L G+G +G A GD NIQD Sbjct: 886 DDTFVASADANSFDGGTGSLHNRVSYAASTAGVTVDLNYSNGTGTSGGYAQGDKLANIQD 945 Score = 55.8 bits (133), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 37/74 (50%), Positives = 47/74 (63%), Gaps = 4/74 (5%) Query: 8 DTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTYTNIQD-X 66 DTF+ANA+ N DG G + VSY +S+ TVNL+TG GSGG A GDTYT+IQ+ Sbjct: 514 DTFVANASVNRLDGGSGGSDTVSYESSNAAVTVNLVTGTGSGGH---AQGDTYTHIQNVV 570 Query: 67 GSVPPTISMAAAAA 80 GS I +A+ A Sbjct: 571 GSAFNDIFVASGDA 584 Score = 54.3 bits (129), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 34/65 (52%), Positives = 42/65 (64%), Gaps = 4/65 (6%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDETVNLITGQGSGGTGSLAAGDTY 60 V G + +D F+A+ AN FDG G+ N VSY NS+V VNL+TG GSGG A GDTY Sbjct: 569 VVGSAFNDIFVASGDANRFDGGAGS-NTVSYVNSAVGVNVNLLTGVGSGGA---ADGDTY 624 Query: 61 TNIQD 65 IQ+ Sbjct: 625 VRIQN 629 Score = 53.9 bits (128), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 37/67 (55%), Positives = 43/67 (64%), Gaps = 6/67 (8%) Query: 1 VTGGSADDTFIANAAANNFDGSGGTHNRVSYANSSVDE--TVNLITGQGSGGTGSLAAGD 58 VTG DDTFIA+ AAN FDG G +N VSYA + TV+L G+GS G AAGD Sbjct: 1007 VTGSGFDDTFIASLAANAFDGGAG-NNTVSYAKAGDGNGVTVDLFNGRGSNG---FAAGD 1062 Query: 59 TYTNIQD 65 TY NIQ+ Sbjct: 1063 TYANIQN 1069 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.309 0.121 0.347 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 333,264 Number of extensions: 10366 Number of successful extensions: 114 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 25 Length of query: 154 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 77 Effective length of database: 1,255,275 Effective search space: 96656175 Effective search space used: 96656175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 126 (53.1 bits)