BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0211.p (361 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 559 e-160 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 138 9e-34 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 132 6e-32 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 120 2e-28 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 559 bits (1440), Expect = e-160, Method: Compositional matrix adjust. Identities = 304/353 (86%), Positives = 323/353 (91%), Gaps = 3/353 (0%) Query: 9 SQAPRTASELLPGLGEIEQAAGIVYAGMAPTPQLCWPLLNQALGAEVWVKHENHAPTGAF 68 S A RT LLP L +IEQAAGIVYAGMAPT QLCWPLLNQALGAEVWVKHENHAPTGAF Sbjct: 2 SHAART---LLPSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAF 58 Query: 69 KVRGGMVYLHHLARQQPQLSGVISATRGNHGQSVGLSARRFGIPATIVVPEGNSREKNAA 128 KVRGGMVYLHHLARQQPQLSGV+SATRGNHGQSVGL+ARR GI ATIVVP+GNSREKNAA Sbjct: 59 KVRGGMVYLHHLARQQPQLSGVVSATRGNHGQSVGLAARRLGISATIVVPQGNSREKNAA 118 Query: 129 MRALGVDLIEHGREFQDSREHAQRLACEQQLHMIPSFHRDLVAGVSTYWMELFAAQPALD 188 MRALGV+LIEHG EFQ+SRE+AQ+LA +QQLHMIPS HRDLVAGV++YWMELFAAQP LD Sbjct: 119 MRALGVELIEHGSEFQESREYAQQLAAQQQLHMIPSLHRDLVAGVASYWMELFAAQPGLD 178 Query: 189 VVLVPIGQGSGMCGAVAARNALGLKTRIIGVVSAHALAYKLSFEAGRKIEAPVSTCIADG 248 VVLVPIGQGSGMCGAVAARNALGL TRIIGVVSAHALAYKLSFEAGRKIE+PVSTCIADG Sbjct: 179 VVLVPIGQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIADG 238 Query: 249 VACRVPDQASLDVMLSEVDEVVAVTDDEVMDAMKLAFIATHNVAEGAGACALAAALQLRT 308 VACRVPDQASL+V+ SEVDEVVAVTDDEVMDAMKLA+IATHNVAEGAGACALAAA QLR Sbjct: 239 VACRVPDQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQLRA 298 Query: 309 RLRGKKIGVTLSGGNVDHDVFASILARPGAAADALLAPAQVVSLAQAAQRRNA 361 RLRG K+GVTLSGGNVDHDV A +LAR A AD LLAPAQV+ L QRR+A Sbjct: 299 RLRGLKVGVTLSGGNVDHDVLARVLARAAAPADMLLAPAQVLPLTSLPQRRSA 351 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 138 bits (347), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 106/324 (32%), Positives = 162/324 (50%), Gaps = 2/324 (0%) Query: 12 PRTASELLPGLGEIEQAAGIVYAGMAPTPQLCWPLLNQALGAEVWVKHENHAPTGAFKVR 71 P +++ LP ++ AA + TP L + LGA+++ K EN GAFK R Sbjct: 3 PVSSATELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFR 62 Query: 72 GGMVYLHHLARQQPQLSGVISATRGNHGQSVGLSARRFGIPATIVVPEGNSREKNAAMRA 131 G M L Q + GVI+ + GNH Q L+A+ GIPA IV+P+ K AA R Sbjct: 63 GAMNALTQFDATQRK-HGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRG 121 Query: 132 LGVDLIEHGREFQDSREHAQRLACEQQLHMIPSF-HRDLVAGVSTYWMELFAAQPALDVV 190 G +++ + R +D RLA E+++ +IP + H ++AG T +EL LD + Sbjct: 122 YGAEVVTYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDAL 181 Query: 191 LVPIGQGSGMCGAVAARNALGLKTRIIGVVSAHALAYKLSFEAGRKIEAPVSTCIADGVA 250 V +G G + GA A AL +I GV + S AG+ + IADG Sbjct: 182 FVCLGGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQ 241 Query: 251 CRVPDQASLDVMLSEVDEVVAVTDDEVMDAMKLAFIATHNVAEGAGACALAAALQLRTRL 310 + + + V+ VD++ V+D+E++ M+ V E G AAA +L+ +L Sbjct: 242 TQHLGKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQL 301 Query: 311 RGKKIGVTLSGGNVDHDVFASILA 334 +GK++GV +SGGNVD A+ LA Sbjct: 302 QGKRVGVIISGGNVDLGKLATFLA 325 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 132 bits (331), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 103/316 (32%), Positives = 165/316 (52%), Gaps = 5/316 (1%) Query: 22 LGEIEQAAGIVYAGMAPTPQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLHHLA 81 L +IE AA + + TP + L+Q +GA+V++K EN T +FK RG L +A Sbjct: 4 LNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLVDVA 63 Query: 82 RQQPQLSGVISATRGNHGQSVGLSARRFGIPATIVVPEGNSREKNAAMRALGVDLIEHGR 141 GV++ + GNH Q V A+R G+PATIV+P K R G +++ HG Sbjct: 64 ASGGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVLHGD 123 Query: 142 EFQDSREHAQRLACEQQLHMI-PSFHRDLVAGVSTYWMELFAAQPALDVVLVPIGQGSGM 200 + +R HA LA ++ L + P ++AG T +E+ A P LD ++V IG G + Sbjct: 124 TLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGGGLL 183 Query: 201 CGAVAARNALGLKTRIIGVVSAHALAYKLSFEAGRKIEAPV-STCIADGVACRVPDQASL 259 G A A+ + I+GV + + A + E PV ++ IA+G+A P + Sbjct: 184 AGMAVAARAIKPEIEIVGVQTRR---FPTMVNAVKGTEHPVGASTIAEGIAVGTPGTLTR 240 Query: 260 DVMLSEVDEVVAVTDDEVMDAMKLAFIATHNVAEGAGACALAAALQLRTRLRGKKIGVTL 319 ++ E DE++ V + ++ A+ + + EGAGA LAA ++ R RGK++G+ L Sbjct: 241 AIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVGLVL 300 Query: 320 SGGNVDHDVFASILAR 335 SGGN+D + +I+ R Sbjct: 301 SGGNIDPLLLTAIIER 316 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 120 bits (301), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 102/325 (31%), Positives = 156/325 (48%), Gaps = 12/325 (3%) Query: 32 VYAGMAPTPQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLHHLARQQPQLSGVI 91 VY TP L+Q + ++ K E+ +FK+RG + HL Q + GVI Sbjct: 14 VYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLK-RGVI 72 Query: 92 SATRGNHGQSVGLSARRFGIPATIVVPEGNSREKNAAMRALGVDLIEHGREFQDSREHAQ 151 A+ GNH Q V LSA R G A IV+P + K A++A G +++ G F D+ EHA Sbjct: 73 CASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHAL 132 Query: 152 RLACEQQLHMIPSFHRDLV-AGVSTYWMELFAAQP-ALDVVLVPIGQGSGMCGAVAARNA 209 L +Q+L + F V AG T ME+ P + + V IG G + G + A Sbjct: 133 TLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKA 192 Query: 210 LGLKTRIIGVVSAHALAYKLSFEAGRKIEAPVSTCIADGVACRVPDQASLDVMLSEVDEV 269 + +IIGV + + A S +AGR++ P +DG A ++ + + + VDEV Sbjct: 193 VRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEV 252 Query: 270 VAVTDDEVMDAMKLAFIATHNVAEGAGACALAAALQLRTR-------LRGKKIGVTLSGG 322 + V D V A+K F T ++ E AGA A+A A R ++G+ + G Sbjct: 253 IIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLITIACGA 312 Query: 323 NVDHD--VFASILARPGAAADALLA 345 N++ D F + +A G A +A+ A Sbjct: 313 NMNFDRLRFVAEMADAGEAREAVFA 337 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,014,477 Number of extensions: 44205 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 4 Length of query: 361 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 275 Effective length of database: 1,212,660 Effective search space: 333481500 Effective search space used: 333481500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)