BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0198.p (208 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313768|ref|YP_003777860.1| acyl carrier protein phosphodie... 119 1e-28 gi|300311442|ref|YP_003775534.1| acyl-carrier-protein phosphodie... 117 8e-28 gi|300313224|ref|YP_003777316.1| [acyl-carrier-protein] phosphod... 103 7e-24 gi|300309901|ref|YP_003773993.1| acyl carrier protein phosphodie... 90 1e-19 >gi|300313768|ref|YP_003777860.1| acyl carrier protein phosphodiesterase [Herbaspirillum seropedicae SmR1] Length = 208 Score = 119 bits (299), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 74/197 (37%), Positives = 107/197 (54%), Gaps = 1/197 (0%) Query: 1 MKILHLLASPRQEASNAWWLSRQIVERLGAQHPQAKVILRDLYASGIAHIDSAYADALGS 60 MKILHL +S EAS + LS +VE L HP A+V +RDL A+ IAH+D A Sbjct: 1 MKILHLDSSILAEASASRVLSAAVVEELRILHPTAQVSVRDLAAAPIAHLDGPIAAGFRQ 60 Query: 61 PVALEDRDTGPGTLALSDRLIEELEQVDQVVIGTPMHNYTVPSVLKAWIDHVVRVRRTFA 120 P A ALS+ L+ + D +V+G PM+N+++PS LKAW+D V + RTF Sbjct: 61 PGANHASPEVRAEQALSETLVSQFLNSDVIVLGVPMYNFSIPSQLKAWLDRVAQPGRTFR 120 Query: 121 ITPDGKTGLLRDRPVHVAVVSGGRFTGASARQPDFLTPYLKVALATIGLHDLRFTVMEGL 180 T +G GL ++ V +A GG ++ A D YLK +G+ +RF E L Sbjct: 121 YTENGPLGLSGEKAVIIASTRGGMYSSGPAAVMDHQEAYLKAFFGFLGIERVRFVRAERL 180 Query: 181 AAGAQAVRNERERVAVE 197 GA+ +R++ R A++ Sbjct: 181 IKGAE-LRSQSMRAALD 196 >gi|300311442|ref|YP_003775534.1| acyl-carrier-protein phosphodiesterase [Herbaspirillum seropedicae SmR1] Length = 202 Score = 117 bits (293), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 65/187 (34%), Positives = 98/187 (52%), Gaps = 1/187 (0%) Query: 3 ILHLLASPRQEASNAWWLSRQIVERLGAQHPQAKVILRDLYASGIAHIDSAYADALGSPV 62 +LH+ +S R S + L+ V + A++P KV+ RDL A+ + H+ + A +P Sbjct: 4 LLHIDSSARSGGSISRQLTASFVSQWQAKNPGGKVVHRDLAANALPHLSESMLGAYFTPA 63 Query: 63 ALEDRDTGPGTLALSDRLIEELEQVDQVVIGTPMHNYTVPSVLKAWIDHVVRVRRTFAIT 122 + + SD L++EL D +VIG PM+N+ PS LKAWIDHV R RTF T Sbjct: 64 DARSAEQAE-VIKQSDALVDELLAADTIVIGVPMYNFAPPSTLKAWIDHVFRAGRTFKYT 122 Query: 123 PDGKTGLLRDRPVHVAVVSGGRFTGASARQPDFLTPYLKVALATIGLHDLRFTVMEGLAA 182 G GL+ + + + GG ++ DF YLK AL IG+ D+ V EG++ Sbjct: 123 ETGPVGLVTGKKAVIILSRGGMYSEGPMEALDFQGKYLKSALGFIGITDVELVVAEGVSM 182 Query: 183 GAQAVRN 189 G +A + Sbjct: 183 GEEAAKQ 189 >gi|300313224|ref|YP_003777316.1| [acyl-carrier-protein] phosphodiesterase [Herbaspirillum seropedicae SmR1] Length = 203 Score = 103 bits (258), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 71/184 (38%), Positives = 97/184 (52%), Gaps = 3/184 (1%) Query: 1 MKILHLLASPRQEASNAWWLSRQIVERLGAQHPQAKVILRDLYASGIAHIDSAYADALGS 60 MK+LHL S E S + LS IV L + P +VI RDL A+ H+ + + A G Sbjct: 1 MKLLHLDTSILGEHSVSRQLSADIVAHLRSLQPDLEVIYRDLAAAAPRHLSAEHLAAFGG 60 Query: 61 PVALEDRDTGPGTLALSDRLIEELEQVDQVVIGTPMHNYTVPSVLKAWIDHVVRVRRTFA 120 A D LAL ++EL Q D +VIG PM+N+++PS LKAWID V TFA Sbjct: 61 --AEVDDPALRADLALGQACLDELLQADLLVIGAPMYNFSLPSQLKAWIDRVAVAGTTFA 118 Query: 121 ITPDGKTGLLRDRPVHVAVVSGGRFTGASARQP-DFLTPYLKVALATIGLHDLRFTVMEG 179 T G GLL+ R ++A V GG++ +A D YL+ L +G+ D+ EG Sbjct: 119 YTDAGPEGLLKGRRAYIASVRGGQYAEGTAEAALDHQERYLRDLLGFLGIVDVSVVRAEG 178 Query: 180 LAAG 183 +A G Sbjct: 179 VAMG 182 >gi|300309901|ref|YP_003773993.1| acyl carrier protein phosphodiesterase [Herbaspirillum seropedicae SmR1] Length = 205 Score = 89.7 bits (221), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 68/203 (33%), Positives = 99/203 (48%), Gaps = 8/203 (3%) Query: 3 ILHLLASPRQEASNAWWLSRQIVERLGAQHPQAKVILRDLYASGIAHIDSAYADALGSPV 62 +L + +SPR S + + +I E + A + +RDL A + HI SAY G Sbjct: 4 VLLITSSPRGADSLSSRFATEIAEGIKASS-AGTLKVRDLIADPLPHITSAYIQ--GRLT 60 Query: 63 ALEDRDTGP-GTLALSDRLIEELEQVDQVVIGTPMHNYTVPSVLKAWIDHVVRVRRTFAI 121 A E+R +AL+ L++EL+ D VVIG+ M N+ S LKAW DHV TFA Sbjct: 61 APENRTPDQVAAVALAQELVDELKAADIVVIGSGMINFGPSSQLKAWFDHVTWPSVTFAY 120 Query: 122 TPDGKTGLLRDRPVHVAVVSGGRFTGASARQPDFLTPYLKVALATIGLHDLRFTVMEGLA 181 +G GLL + ++ +GG F+ DF + YL L+ IGL D+ +EG Sbjct: 121 DDNGPKGLLTGKKTYLVAAAGGVFSEGIWAAFDFQSNYLLHLLSFIGLSDVEMVCVEGTV 180 Query: 182 AGAQAVR----NERERVAVELDR 200 G A + N V V L++ Sbjct: 181 FGPDAAKAAIANTEAAVQVLLNK 203 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 712,303 Number of extensions: 32142 Number of successful extensions: 102 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 4 Length of query: 208 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 127 Effective length of database: 1,236,335 Effective search space: 157014545 Effective search space used: 157014545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)