BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0194.p (480 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 665 0.0 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 401 e-113 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 375 e-105 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 361 e-101 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 263 2e-71 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 665 bits (1716), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/453 (80%), Positives = 400/453 (88%), Gaps = 7/453 (1%) Query: 20 VACQSMAPDYQRPAAPVAAHYPQ-------QSDGQSEAAPALGWQAWFRDPQLQALITQA 72 ACQS+AP YQRPAAPVAA YP A+P L W+ +F DP LQALITQA Sbjct: 23 TACQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQA 82 Query: 73 LANNRDLRAAALRVEEARALYGIQRADLFPAVGAQAGLDRSRTPADLNLTGRPLVGSAYQ 132 L N+RDLR AALRV+EA+ALYGI ADLFP +GAQAGLDRSRTPADLNLT RPLVGSAYQ Sbjct: 83 LENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ 142 Query: 133 AGVGLSTWELDLWGRLRSLDDAALHTALASEATRRAAALSLVAQVADAWLELAEIDERLA 192 AG+GLS+WE+DLWGRLRSLDDAAL +A AS+ATR A ALSL+AQVADAWLELAE+DERL Sbjct: 143 AGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLE 202 Query: 193 IARDTIASRQESYRIFARRVEVGATSRLNLTQIETLLTQAQALGAQLEQQRAQRLNALTL 252 IA TIASRQES+RIF+RRVEVGATSRLNLTQIETLLTQAQALGAQL+QQRAQRLNALTL Sbjct: 203 IANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTL 262 Query: 253 LVGSPLELPVGQARPALDAAFPALSPGTPSLLLTRRPDVAAAEHRLQAANANIGAARAAF 312 LVGSP+ LPVGQAR LDA F L PG P+LLLTRRPDVAAAEHRL AANANIGAARAAF Sbjct: 263 LVGSPVVLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAF 322 Query: 313 FPSISLTGNLGTASAELSGLFRDGSHAWSFSPSLSLPIFTAGRLRNNLNLAEVRRDLAVA 372 FPSISLTGN+GTASAELSGLFRDGSHAW+FSPS++LPIFTAGRLRNNL L+EVRRDLAVA Sbjct: 323 FPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVA 382 Query: 373 NYEKTIQSAFRDVADALAAHQWLSRQLAIAETAEQVQAERARLSTLRYDNGAAPFLDVLD 432 YEKT+Q+AFR+VADALAA QWL++QL IA++A +VQ ERARLS LRYDNGAAPFLDVLD Sbjct: 383 AYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLD 442 Query: 433 AQRDLLSARQQRVQIRRALLSSSVALYAALGGD 465 AQRDLLSA+QQ VQIRRALLSSSVALYAALGG+ Sbjct: 443 AQRDLLSAQQQLVQIRRALLSSSVALYAALGGE 475 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 401 bits (1030), Expect = e-113, Method: Compositional matrix adjust. Identities = 234/467 (50%), Positives = 308/467 (65%), Gaps = 10/467 (2%) Query: 7 RIRPLLAALPL--LLVACQSMAPDYQRPAAPVAAHYPQQSDGQSE-AAPALGWQAWFRDP 63 R+ + AAL + +L C S+AP Y+RP APV + YP + G++E A LGW+ +F D Sbjct: 11 RLLHMCAALVMAGVLGGC-SLAPTYERPQAPVESSYPADAAGRAELQAVNLGWRQFFPDQ 69 Query: 64 QLQALITQALANNRDLRAAALRVEEARALYGIQRADLFPAVGAQAGLDRSRTPADLNLTG 123 +LQ LI AL NNRDLR A L +EEARA Y I RADL P + R+RT A +LTG Sbjct: 70 RLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTG 129 Query: 124 RPLVGSAYQAGVGLSTWELDLWGRLRSLDDAALHTALASEATRRAAALSLVAQVADAWLE 183 +P + ++YQ G+ + +ELD +GR+R+L DAAL + L++E +++A +SLVAQVA A+L Sbjct: 130 QPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLA 189 Query: 184 LAEIDERLAIARDTIASRQESYRIFARRVEVGATSRLNLTQIETLLTQAQALGAQLEQQR 243 E+L +A +T+ SRQ + + +R++VGA++ L+ +TL+ A+ QLE+QR Sbjct: 190 ERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQR 249 Query: 244 AQRLNALTLLVGSPL-ELPVGQ--ARPALDAAFPALSPGTPSLLLTRRPDVAAAEHRLQA 300 AQ NALTLLVG + ELP Q + + PA G PS LLT RPD+ +AE L A Sbjct: 250 AQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPA---GLPSDLLTNRPDIRSAEQSLLA 306 Query: 301 ANANIGAARAAFFPSISLTGNLGTASAELSGLFRDGSHAWSFSPSLSLPIFTAGRLRNNL 360 ANANIGAARAAFFP ISLTGN G+AS+ L GLF GS AWSF PSLSLPIF GR NL Sbjct: 307 ANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANL 366 Query: 361 NLAEVRRDLAVANYEKTIQSAFRDVADALAAHQWLSRQLAIAETAEQVQAERARLSTLRY 420 +LAEVR++ AV YEK IQ+AFR+VADAL A L Q+ E Q ER +L+ LR+ Sbjct: 367 DLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRF 426 Query: 421 DNGAAPFLDVLDAQRDLLSARQQRVQIRRALLSSSVALYAALGGDSN 467 NG A LD LDAQRDL SA+Q +Q R+ L++++ LY ALGG N Sbjct: 427 KNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLN 473 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 375 bits (962), Expect = e-105, Method: Compositional matrix adjust. Identities = 227/484 (46%), Positives = 299/484 (61%), Gaps = 26/484 (5%) Query: 2 CEPSLRIR-PLL-----AALPLLLVACQSMAPDYQRPAAPVAAHY---PQQSDGQSEAAP 52 +PSL R P+L L +LL C M PDY RP APVAAH+ ++ + +AP Sbjct: 1 MKPSLSTRSPVLPMMLPVMLAMLLGGCTLM-PDYHRPDAPVAAHFAGADSKNAAGASSAP 59 Query: 53 A---LGWQAWFRDPQLQALITQALANNRDLRAAALRVEEARALYGIQRADLFPAVGAQAG 109 +GW+ F DP LQ +I AL NNRDLR A L +E+ARA YG+QRA LFP++ A Sbjct: 60 VVADIGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATS 119 Query: 110 LDRSRTPADLNLTGRPLVGSAYQAGVGLSTWELDLWGRLRSLDDAALHTALASEATRRAA 169 SRTP DL+ TG L +Y A +G S +ELDL+GR+RSL + AL + L++ RR+ Sbjct: 120 ETASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRST 179 Query: 170 ALSLVAQVADAWLELAEIDERLAIARDTIASRQESYRIFARRVEVGATSRLNL----TQI 225 +SL+A+VA AWL LA +RL +A+DT+ S+ SY + R E+G++S L L T + Sbjct: 180 QISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSV 239 Query: 226 ETLLTQAQALGAQLEQQRAQRLNALTLLVGSPLE---LPVGQARPALDAAFP--ALSPGT 280 E+ ++ AQ++Q R NAL LLVG L LP G L AA P + PG Sbjct: 240 ESARVDVESYTAQVDQDR----NALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGL 295 Query: 281 PSLLLTRRPDVAAAEHRLQAANANIGAARAAFFPSISLTGNLGTASAELSGLFRDGSHAW 340 PS LL RR D+ AE L+AANANIGAARAAF+PSI+LT N GTASA L+GLF+ GS +W Sbjct: 296 PSDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSW 355 Query: 341 SFSPSLSLPIFTAGRLRNNLNLAEVRRDLAVANYEKTIQSAFRDVADALAAHQWLSRQLA 400 SF P +SLPIF G + NL++A V RD++VA YEK IQ+AFR+V+DALA L RQL Sbjct: 356 SFVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQ 415 Query: 401 IAETAEQVQAERARLSTLRYDNGAAPFLDVLDAQRDLLSARQQRVQIRRALLSSSVALYA 460 E Q E RLS RY +G +L VLD+QR L SA++ + + + ++ V Y Sbjct: 416 AQEALVQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYK 475 Query: 461 ALGG 464 LGG Sbjct: 476 TLGG 479 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 361 bits (927), Expect = e-101, Method: Compositional matrix adjust. Identities = 215/457 (47%), Positives = 284/457 (62%), Gaps = 16/457 (3%) Query: 22 CQSMAPDYQRPAAPVAAHYPQQSDGQ---SEAAPA--------LGWQAWFRDPQLQALIT 70 C ++APDY +PAAPV A + Q+ Q A PA LGW+ +F DP+LQ +I Sbjct: 28 CANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVIA 87 Query: 71 QALANNRDLRAAALRVEEARALYGIQRADLFPAVGAQAGLDRSRTPADLNLTGRPLVGSA 130 AL NNRDLR AAL +E+ARA Y I A+ +P++ A SRTPA+L+ TG V Sbjct: 88 AALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSHQ 147 Query: 131 YQAGVGLSTWELDLWGRLRSLDDAALHTALASEATRRAAALSLVAQVADAWLELAEIDER 190 VG+S +ELD +GRL++L DAAL + TRR+ +SLVA+VA AWL LA + Sbjct: 148 QSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDL 207 Query: 191 LAIARDTIASRQESYRIFARRVEVGATSRLNLTQIETLLTQAQALGAQLEQQRAQRLNAL 250 L +A+DT AS+ ++ + R ++G S ++L E + A+A A+ Q Q LNAL Sbjct: 208 LKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNAL 267 Query: 251 TLLVGSPLE---LPVGQARPALDAAFPALSPGTPSLLLTRRPDVAAAEHRLQAANANIGA 307 LLVG+ ++ LP G P + PA G PS +L RRPDV AAEH L++ANA+IGA Sbjct: 268 RLLVGADIDAALLPAG--LPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGA 325 Query: 308 ARAAFFPSISLTGNLGTASAELSGLFRDGSHAWSFSPSLSLPIFTAGRLRNNLNLAEVRR 367 ARAAFFPSISLT N GTAS L GLF+ GS +W+FSPS++LPIF AG LR +L+ A++ R Sbjct: 326 ARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITR 385 Query: 368 DLAVANYEKTIQSAFRDVADALAAHQWLSRQLAIAETAEQVQAERARLSTLRYDNGAAPF 427 D+ VANYEK IQ+AFR+VADALA L ++L A + RLS RY NG + Sbjct: 386 DIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSY 445 Query: 428 LDVLDAQRDLLSARQQRVQIRRALLSSSVALYAALGG 464 L L AQR L SA+Q + +R S+ + LY LGG Sbjct: 446 LTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGG 482 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 263 bits (673), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 189/469 (40%), Positives = 264/469 (56%), Gaps = 12/469 (2%) Query: 6 LRIRPLLAALPLLLVACQSMAPDYQRPAAPVAAHYPQQ--SDGQSEAAP---ALGWQAWF 60 LR+ P LA L AC + A + HYP+ D + AAP A+GWQ +F Sbjct: 9 LRLLPTLA-----LCACAPLTAPLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDYF 63 Query: 61 RDPQLQALITQALANNRDLRAAALRVEEARALYGIQRADLFPAVGAQAGLDRSRTPADLN 120 LQALI +AL +N DLR LR+++ +A + IQR+ P V +A RSR P + Sbjct: 64 PQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPP 123 Query: 121 LTGRPLVGSAYQAGVGLSTWELDLWGRLRSLDDAALHTALASEATRRAAALSLVAQVADA 180 +G Y+ G+G S+WELD WGR+ SL++AAL +AS A +A +SL+ QVA A Sbjct: 124 ESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAA 183 Query: 181 WLELAEIDERLAIARDTIASRQESYRIFARRVEVGATSRLNLTQIETLLTQAQALGAQLE 240 W L E+DERL + A+ ES + R + G L Q + L Q +A A+L+ Sbjct: 184 WTSLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQ 243 Query: 241 QQRAQRLNALTLLVGSPLELPVGQARPALDAAFP-ALSPGTPSLLLTRRPDVAAAEHRLQ 299 Q RA + + L +L G +LP P AL+PG PS LL RPD+ AAEHRLQ Sbjct: 244 QMRASQRSTLEMLAGL-TQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIAAEHRLQ 302 Query: 300 AANANIGAARAAFFPSISLTGNLGTASAELSGLFRDGSHAWSFSPSLSLPIFTAGRLRNN 359 A+ A + AARAAFFP ISLT G AS L LF+ S AW+F P +SLP+ G ++ Sbjct: 303 ASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSR 362 Query: 360 LNLAEVRRDLAVANYEKTIQSAFRDVADALAAHQWLSRQLAIAETAEQVQAERARLSTLR 419 ++A + + A+ +YE+T+Q+AFR+VA L WL QL + Q QA+R +R Sbjct: 363 YDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMR 422 Query: 420 YDNGAAPFLDVLDAQRDLLSARQQRVQIRRALLSSSVALYAALGGDSNS 468 G A LDVL+++RD L A+Q++ + ALL + +ALY ALGG + + Sbjct: 423 LRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQA 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.130 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,271,919 Number of extensions: 45761 Number of successful extensions: 285 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 19 Length of query: 480 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 391 Effective length of database: 1,198,455 Effective search space: 468595905 Effective search space used: 468595905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)