BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0028.0193.p
         (356 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311156|ref|YP_003775248.1| type I antifreeze protein:HlyD ...   548   e-157
gi|300311270|ref|YP_003775362.1| HlyD family membrane-fusion pro...   294   6e-81

>gi|300311156|ref|YP_003775248.1| type I antifreeze protein:HlyD
           family secretion protein [Herbaspirillum seropedicae
           SmR1]
          Length = 360

 Score =  548 bits (1412), Expect = e-157,   Method: Compositional matrix adjust.
 Identities = 289/332 (87%), Positives = 307/332 (92%), Gaps = 2/332 (0%)

Query: 27  WLRLHRSGPGEGFASGNGRIEATEVDVATKLGGRVDAILVNEGDFVAAGQPLARMQVLSL 86
           W RLH +GPGEGF SGNGRIEATEVDVATKLGGRVDAILVNEGDFV+AGQPLARMQVLSL
Sbjct: 29  WQRLHHNGPGEGFTSGNGRIEATEVDVATKLGGRVDAILVNEGDFVSAGQPLARMQVLSL 88

Query: 87  EAQRDEAQARQQQSVSAVASAEAQVAVRESDHQAALAQAAQRARSLXG--RRLARSEVLV 144
           +AQRDEA A QQQS+SAVASAEAQVAVR+SD+QAALAQ  QR   L    RRLARSEVLV
Sbjct: 89  QAQRDEALAHQQQSLSAVASAEAQVAVRQSDYQAALAQTVQRESELDAARRRLARSEVLV 148

Query: 145 KEGASSLQEVDDDRARVLSMEAAVTAAKAQVTAAQAAVAAARTQVSGARATVTAAVATVQ 204
           +EGASS+QEVDDDRARV SMEAAVTA+KAQVTAA+AAV AARTQVSGAR+TVTAA ATVQ
Sbjct: 149 REGASSVQEVDDDRARVRSMEAAVTASKAQVTAAEAAVVAARTQVSGARSTVTAAQATVQ 208

Query: 205 RIKVDIDDSTLVAPRAGRVQYRIAQPGEVLGGGGKLLNLVDLSDVYMTFFVPETVAGKLA 264
           RIKVDIDDSTL APRAGRVQYRIAQPGEVLGGGGK+LNLVDLSDVYMTFF+PE V GKLA
Sbjct: 209 RIKVDIDDSTLAAPRAGRVQYRIAQPGEVLGGGGKVLNLVDLSDVYMTFFLPEVVVGKLA 268

Query: 265 LGGEARIVLDAAPQYVIPARISFVASTAQFTPKTVETASERQKLMFRVKAQIDPALLRQH 324
           LG EARI+LDAAPQYVIPARISFVASTAQFTPKTVETASERQKLMFRVKAQIDPALL+QH
Sbjct: 269 LGSEARIILDAAPQYVIPARISFVASTAQFTPKTVETASERQKLMFRVKAQIDPALLQQH 328

Query: 325 LSLVKTGLPGVAWVRTDNARDWPATLAVKVPQ 356
           L+LVKTGLPGVAWVRTD  + WPA LAV+VPQ
Sbjct: 329 LTLVKTGLPGVAWVRTDQNQPWPAQLAVQVPQ 360


>gi|300311270|ref|YP_003775362.1| HlyD family membrane-fusion
           protein [Herbaspirillum seropedicae SmR1]
          Length = 358

 Score =  294 bits (753), Expect = 6e-81,   Method: Compositional matrix adjust.
 Identities = 172/348 (49%), Positives = 232/348 (66%), Gaps = 6/348 (1%)

Query: 7   KKPLLAAALAVILA--GAAAYAWLRLHRSGPGEGFASGNGRIEATEVDVATKLGGRVDAI 64
           + PL A  L V+LA  G   +AW R     P  G  S NGR+EATEVD+ ++LGGR+  +
Sbjct: 6   QHPLFAGLLLVVLAAVGWTGWAWWR-DADAPA-GLVSSNGRLEATEVDIGSRLGGRIRHM 63

Query: 65  LVNEGDFVAAGQPLARMQVLSLEAQRDEAQARQQQSVSAVASAEAQVAVRESDHQAALAQ 124
           LV+EG+ V AGQ LA +QV SL AQ  EA+A+QQ++   V  A+AQ+A+R S+ + A A 
Sbjct: 64  LVDEGEAVQAGQVLAYLQVDSLHAQLREARAQQQRASQGVTVAQAQLAMRRSEQRIAQAL 123

Query: 125 AAQRARSLXG--RRLARSEVLVKEGASSLQEVDDDRARVLSMEAAVTAAKAQVTAAQAAV 182
             QR +SL    RR+ RS+ L +  A + QEVDD+   +    A + AA+AQ   A+AA+
Sbjct: 124 TVQRHKSLSAATRRVERSQPLGRMQALTAQEVDDEENTLAEARAQLDAAQAQEEGARAAI 183

Query: 183 AAARTQVSGARATVTAAVATVQRIKVDIDDSTLVAPRAGRVQYRIAQPGEVLGGGGKLLN 242
            AA   V+ ARA + AA A   RI+ D+++S + APR G +QYR+AQ GEV+  G KLLN
Sbjct: 184 RAAEADVASARALLEAAQAATARIEADLEESAIRAPRDGHIQYRLAQAGEVVSEGSKLLN 243

Query: 243 LVDLSDVYMTFFVPETVAGKLALGGEARIVLDAAPQYVIPARISFVASTAQFTPKTVETA 302
           L+DLSD++M FF+P  +AGK+ LG E R+VLDAAP   +PARI++V+  AQFTPK VETA
Sbjct: 244 LIDLSDLHMHFFLPAELAGKVRLGQEVRVVLDAAPTDALPARITYVSRQAQFTPKAVETA 303

Query: 303 SERQKLMFRVKAQIDPALLRQHLSLVKTGLPGVAWVRTDNARDWPATL 350
           +ERQKLMFRVKAQ+ P+        +K G+PGVAW++T     WP TL
Sbjct: 304 NERQKLMFRVKAQLTPSSRGSIAPHLKAGMPGVAWIKTAEQLPWPKTL 351


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.128    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 711,027
Number of extensions: 22405
Number of successful extensions: 142
Number of sequences better than 1.0e-08: 5
Number of HSP's gapped: 138
Number of HSP's successfully gapped: 5
Length of query: 356
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 270
Effective length of database: 1,212,660
Effective search space: 327418200
Effective search space used: 327418200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)