BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0184.p (460 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 807 0.0 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 142 6e-35 gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 122 7e-29 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 101 1e-22 gi|300310674|ref|YP_003774766.1| amidase family protein [Herbasp... 99 7e-22 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 807 bits (2084), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/450 (92%), Positives = 435/450 (96%) Query: 11 MPSTLSQLAADLDAGRITSLQLTEQALARIADPAGEGARVFTSVYADQARAAARASDTLR 70 MPSTLS+LAADL AG TSLQLTEQALARIADPAGEGARVFT VYA+QARAAARASDTLR Sbjct: 1 MPSTLSRLAADLAAGHTTSLQLTEQALARIADPAGEGARVFTRVYAEQARAAARASDTLR 60 Query: 71 AAKLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVIGK 130 AA+LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKP A+AHAAVVQNL+KAGAI+IGK Sbjct: 61 AARLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGK 120 Query: 131 TNMTEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG 190 TNMTEFAYSGLGINPHYGTPQN WERN+EGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG Sbjct: 121 TNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG 180 Query: 191 SVRIPSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECCAIMDAVLAGEAYVS 250 SVRIPSALNGLTGFKPTA+RVSMQGVLPLSAYLDSIGPLAVSV+CCAIMDAVLAGE YV+ Sbjct: 181 SVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVA 240 Query: 251 VVAHPLRGLRLLAPTNVVLDAMDETVGKAYRAALDKLRAAGAVIVEAEVPAFNALAAINA 310 V+AHPLRGLRLLAPTNVVLD MD+TVG+AYR AL KL AAGA+IVEAEVPAFNALAAINA Sbjct: 241 VLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINA 300 Query: 311 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAEVQAQ 370 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIA+V+AQ Sbjct: 301 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQ 360 Query: 371 LAGYDALVMPTVPIVAPRIAELQASDDAYYAANGKILRNPTLINFLDGCAVSLPCHAPGT 430 LAGYDALVMPTVPIVAPRIAELQASDDAYY ANGK+LRNPTLINFLDGCA+SLPCHAPG+ Sbjct: 361 LAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGS 420 Query: 431 APVGLSLAAAGGEDKRLLAIALAVEGLLAA 460 APVGLSLAA GG+DKRLL+I LAVE LLAA Sbjct: 421 APVGLSLAACGGQDKRLLSIGLAVEALLAA 450 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 142 bits (358), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 141/432 (32%), Positives = 209/432 (48%), Gaps = 52/432 (12%) Query: 57 DQARAAARASDTLRAAKLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAV 116 +Q R+ AR D + PL G+P+++KD DVAG + AG+ LR HA V Sbjct: 48 EQLRSQARMLDE----QGGHGPLSGIPIAIKDNIDVAGIASTAGTAALRNN-VPQRHATV 102 Query: 117 VQNLMKAGAIVIGKTNMTEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVT 176 V+ L AGA+ GK NM E A+ N +G +N ++ + I GGSS G + Sbjct: 103 VEYLFGAGALHAGKANMHELAFGITNNNGVFGPCRNPYDAEL----IAGGSSGGCGALLA 158 Query: 177 DGMAFAAVGSDTGGSVRIPSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECC 236 +A AAVG+DTGGSVRIP+AL G+ G +PT+ R S GV+P+S D+ GP+A SV Sbjct: 159 ARLAPAAVGTDTGGSVRIPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADV 218 Query: 237 AIMDAVLAG-EAYVSVVAHPLRGLRLLAPTNVVLDAMDETVGKAYRAALDKLRAAGAVIV 295 A++D ++AG ++A P LRL N ++E V AAL +LR AG +V Sbjct: 219 ALLDQIMAGSRDSAPLLALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELV 278 Query: 296 EAEVPAFNALAAINAKGGFTAAEAYAWHRALI---AENAAGYDQRVVSRILRGKDMSAAD 352 + ++P LA ++ GF A H LI A A + V+ R+L ++ + D Sbjct: 279 DVDLP---TLAPLSHAAGF----AIVLHEFLIDLPAYLQASDARDVIKRVL--AEVGSPD 329 Query: 353 LLDVLHAR-PRWIAEVQAQLA------------------GYDALVMPTVPIVAPRIAELQ 393 + ++ A + + E +LA G DAL+ PT + A + E Sbjct: 330 VARIIQATLDQPVPEHAYRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGE-- 387 Query: 394 ASDDAYYAANGKILRNPTLINFLDGC------AVSLPCHAPGTA-PVGLSLAAAGGEDKR 446 DD ++ P DG ++S+P PVGL L G D+R Sbjct: 388 --DDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRR 445 Query: 447 LLAIALAVEGLL 458 LL +A ++E +L Sbjct: 446 LLQVAASLETVL 457 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 122 bits (305), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 142/482 (29%), Positives = 216/482 (44%), Gaps = 57/482 (11%) Query: 14 TLSQLAADLDAGRITSLQLTEQALARIADPAGEGARVFTSVYADQARAAARASDTLRAAK 73 TL +L+A L A +I++ +L L RI D + A F V + A A+ +D+ R + Sbjct: 5 TLKELSAQLQARKISATELATLFLDRI-DASDLNA--FLHVDRELTLAQAKVADS-RLSD 60 Query: 74 LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVIGKTNM 133 L GVP++ KD+F G T AGS +L + A VV+ AG + +GK N Sbjct: 61 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNY-VSPFDATVVEQFNAAGMVTLGKLNC 119 Query: 134 TEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVR 193 EFA N ++G N W++ G SS G+A +V +A AA +DTGGS+R Sbjct: 120 DEFAMGSGNENSYFGPTLNPWDKRAVPGG----SSGGSAAAVAARLAPAATATDTGGSIR 175 Query: 194 IPSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECCAIMDAVLAG-------- 245 P++L G+TG KPT VS G++ ++ LD GP+A + E C ++ + G Sbjct: 176 QPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTS 235 Query: 246 -----EAYVSVVAHPLRGLRLLAPTNVVLDAMDETVGKAYRAALDKLRAAGAVIVEA--- 297 E + + L+GLR+ P + V A RAAL + GA +VE Sbjct: 236 LQRPKEDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLP 295 Query: 298 ----EVPAFNALAAINAKGGFTAAEAYAW-HRALIAEN---------AAGYDQRVVSRIL 343 +P + +A A + + + HRA N A G+ + V RIL Sbjct: 296 KTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRIL 355 Query: 344 RGKDMSAADLLDVLHARPRWIAEVQAQ-----LAG----YDALVMPTVPIVAPRIAELQA 394 G + + D + + + I + AQ LAG D ++ P P VA + + Sbjct: 356 VGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDKTN 415 Query: 395 SDDAYYAANGKILRNPTLINFLDGCAVSLPC-----HAPGTAPVGLSLAAAGGEDKRLLA 449 A Y A+ I T + L G +S+PC G PVGL + ++ RLL Sbjct: 416 DPVANYLAD--IFTLSTSLAGLPG--MSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLN 471 Query: 450 IA 451 +A Sbjct: 472 VA 473 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 105/320 (32%), Positives = 150/320 (46%), Gaps = 34/320 (10%) Query: 10 IMPSTLSQLAADLDAGRITSLQLTEQALARIADPAGEGARVFTSVYAD-QARAAARASDT 68 + ++++ L + DAGR TS L LARI +G + V + +A A D Sbjct: 49 VFEASITHLQSAQDAGRATSRSLVLAYLARIRAYDQQGPSLNAIVTLNPKALEEADQLDR 108 Query: 69 LRAAKLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVI 128 R R PL G+P+ VKD +D T G++ L A A A V+ L +AGA+++ Sbjct: 109 ERRQSGPRGPLHGIPILVKDNYDTVDMPTTGGTLAL-ATLQAQADAFQVKRLREAGAVIL 167 Query: 129 GKTNMTEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDT 188 GKT M E A ++ G +N ++ R PGGSS G +V A A +GSDT Sbjct: 168 GKTTMHELAAGVTTVSSLTGFTRNPYDPR----RAPGGSSGGTGAAVAASFAAAGMGSDT 223 Query: 189 GGSVRIPSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECCAIM-DAVLAGEA 247 GS+RIP+A L G + T S GV+PLS+ D PLA SVE AIM DA + + Sbjct: 224 CGSIRIPAAHQNLFGLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDP 283 Query: 248 YVS----------------VVAHPLRGLRLLAPTNVVLDAM------DETVGKAYRAALD 285 S + A L+G R+ VL A+ D V A AL Sbjct: 284 QDSSTVDANGHIPKSYRDGLRADSLQGARI-----GVLRALFGAAPEDAEVSAAINKALQ 338 Query: 286 KLRAAGAVIVEAEVPAFNAL 305 +L+ GA++ + +P + L Sbjct: 339 QLKDQGAIVTDVTIPELDGL 358 >gi|300310674|ref|YP_003774766.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 99.0 bits (245), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 62/154 (40%), Positives = 86/154 (55%), Gaps = 4/154 (2%) Query: 81 GVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVIGKTNMTEFAYSG 140 G V++KD D+AG+ T GS L P A+AHA VV L++AG + + NM E AY Sbjct: 26 GPTVAIKDCIDIAGQPTCGGSAALADAPPASAHADVVARLLEAGWQITARANMHELAYGM 85 Query: 141 LGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALNG 200 GIN GT N + G GS+S + D ++GSDTGGS+R+P+A G Sbjct: 86 TGINEWNGTAVNPQDPACMTGGSSSGSASAVGAGLVD----VSIGSDTGGSIRLPAACCG 141 Query: 201 LTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVE 234 + GFKP+ RVS G P + LD +GP + S++ Sbjct: 142 VIGFKPSFGRVSRAGAYPALSSLDCLGPFSRSMD 175 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,412,441 Number of extensions: 58968 Number of successful extensions: 270 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 245 Number of HSP's successfully gapped: 11 Length of query: 460 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 372 Effective length of database: 1,203,190 Effective search space: 447586680 Effective search space used: 447586680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)