BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0181.p (437 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 776 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 444 e-126 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 393 e-110 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 343 2e-95 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 338 8e-94 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 776 bits (2005), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/437 (94%), Positives = 427/437 (97%) Query: 1 MSYSPSAAATAAPPASSSASLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 MSYSPSAAAT A AS++A LE TY KIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD Sbjct: 1 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 Query: 61 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPI 120 LQFSEAVYGLGAGIFFIGYF+FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTP Sbjct: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 Query: 121 SFYVLRFLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLIMS 180 SFY+LRFLLGIFEAGFFPGVVLYLTYWFPA+RRARVNGLFMTSFAIAGAVGGPIAGLIMS Sbjct: 121 SFYILRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMS 180 Query: 181 SMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAENS 240 MEGVGHLANWQWLFI+EGIPSILAGFAVLAWLPE+PANAKWLS+AEK AVTQVLAAENS Sbjct: 181 GMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENS 240 Query: 241 APGKHFSFKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP 300 APGKHFSFKDACTNYRVW+CAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP Sbjct: 241 APGKHFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP 300 Query: 301 FVAGTLAMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAIGI 360 F+AGTLAM+WNGFHSDKTGERRLHSAIATLIACIGL+ATGMTLGNATLALCSLTLAAIGI Sbjct: 301 FIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGI 360 Query: 361 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 420 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA Sbjct: 361 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 420 Query: 421 ALEFLASFLVVVFVKKH 437 ALE +ASFLVVVF++KH Sbjct: 421 ALELVASFLVVVFIRKH 437 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 444 bits (1142), Expect = e-126, Method: Compositional matrix adjust. Identities = 217/400 (54%), Positives = 289/400 (72%), Gaps = 1/400 (0%) Query: 22 EATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFL 81 +ATY K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FS+ VYGLGAGIFF+GYF+ Sbjct: 18 DATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 Query: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPISFYVLRFLLGIFEAGFFPGVV 141 FEVPSN++L K+GAR +ARI I WG+ S AMAYVTTP FY++RFLLG+ EAGFFPGV+ Sbjct: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVI 137 Query: 142 LYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLIMSSMEGVGHLANWQWLFIIEGIP 201 LYLTYW+PA RR ++ LFMT A++G +GGP++G IM +M GV WQW+FI+E IP Sbjct: 138 LYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIP 197 Query: 202 SILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAENSAPGKHFSFKDACTNYRVWVCA 261 S++ G V+ ++ ++ +A WLS+ EK + ++ E S +H S N RVW+ A Sbjct: 198 SLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQK-EHLSLGAMFANGRVWLGA 256 Query: 262 AVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFVAGTLAMVWNGFHSDKTGER 321 +YFC V G ++FW P+IIK GV L +GL++AIP+ AMV G +D ER Sbjct: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 Query: 322 RLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAA 381 R H AI I CIGL+ + N TLA+ SLTLA++GIL P+FWS+P AFL+GTAAA Sbjct: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 Query: 382 GGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAA 421 GIALINS+GNLAGFV+P+++G LK +T S ++G+Y +AA Sbjct: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAA 416 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 393 bits (1009), Expect = e-110, Method: Compositional matrix adjust. Identities = 199/422 (47%), Positives = 279/422 (66%) Query: 6 SAAATAAPPASSSASLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSE 65 +A+ T + S + Y K+ WR++PFL+ +V+A++DRVNVGFAKLQM QDL FSE Sbjct: 3 TASTTGVGLDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSE 62 Query: 66 AVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPISFYVL 125 V+GLGAG+FFIGYFLFEVPSN+L+ K+GAR +ARI I WG+ S A +V FY+L Sbjct: 63 TVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYIL 122 Query: 126 RFLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLIMSSMEGV 185 RFLLG+ EAGF+PG++LYLTYWFP+ RRA+V +FM+ +AG +G P++G IM + Sbjct: 123 RFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQN 182 Query: 186 GHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAENSAPGKH 245 G L WQW+F+IE IP++L G A + +L +AKWL+D EK ++ + + Sbjct: 183 GGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESK 242 Query: 246 FSFKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFVAGT 305 F + RVW+ +YF V G + W P+++K GVKGNL+IGL+SAIPF Sbjct: 243 HGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAI 302 Query: 306 LAMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAIGILAAFP 365 +AM G +D+ ERR H I L+ +G + + N +++ SL+LAA G+L P Sbjct: 303 IAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAP 362 Query: 366 VFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALEFL 425 +FWS+P AFL+G AAA GIA INS+GNLAGFV+PY+IG LK T + ++G+Y +A + + Sbjct: 363 LFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVV 422 Query: 426 AS 427 S Sbjct: 423 GS 424 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 343 bits (880), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 179/419 (42%), Positives = 265/419 (63%), Gaps = 7/419 (1%) Query: 1 MSYSPSAAATAAPPASSSASLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 M+ S A + P+ +S + +A Y K+ R+IPFL +V++++DR+N+GFA+LQM QD Sbjct: 1 MTPSSGGATLSTTPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQD 60 Query: 61 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPI 120 L FS+A+YGLGA IF++GY LFEVPSN+ L + GAR+T +RI +LWGLAS+ M VT Sbjct: 61 LGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAA 120 Query: 121 SFYVLRFLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLIMS 180 FY LRFLLG+FEAGFFPG+VLYL+YWFPA+RRA V +F A+AG +GG ++G IM Sbjct: 121 HFYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMR 180 Query: 181 SMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAENS 240 M GV L WQW+F IEG P+++ G + L + PA AKWLS E++ + Q + + Sbjct: 181 DMAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFL-QARHQQEA 239 Query: 241 APGKHFS------FKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIG 294 H S + A ++ ++ A +YF + G+ T+ FW P +I++ G+K +++ Sbjct: 240 LRTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVS 299 Query: 295 LISAIPFVAGTLAMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLT 354 L S IP G + ++ SD+ ER H A+ T + L A + + ++L L+ Sbjct: 300 LYSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLS 359 Query: 355 LAAIGILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLS 413 +AA+ I AA P+FWS+P L A GIA I+S+G +G V+P++IG +KT TG++ Sbjct: 360 VAAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMD 418 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 338 bits (866), Expect = 8e-94, Method: Compositional matrix adjust. Identities = 191/431 (44%), Positives = 269/431 (62%), Gaps = 12/431 (2%) Query: 17 SSASLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFF 76 S+ + Y K+ W+++P+L+ +V+A++DRVNVGFAKL ML DL SEA YGLGAGIFF Sbjct: 8 SAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFF 67 Query: 77 IGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPIS----------FYVLR 126 +GYF+FEVPSNL+L ++GA+ +ARI I WG+ S MA+ T PI+ FY LR Sbjct: 68 LGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAF-TAPIAQWLGVSNETVFYTLR 126 Query: 127 FLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLIMSSMEGVG 186 FLLG EAGFFPGV+LY+TYW+PA +RV + + + +GGPI+G +M M Sbjct: 127 FLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGD 186 Query: 187 HLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAENSAPGKHF 246 LA WQW+F+IE +P+ L G +L L A+AKWL+ EK + + LAAEN + Sbjct: 187 SLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEK-VNV 245 Query: 247 SFKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFVAGTL 306 K +W A VY ++ G+ I FWAP+II++ GVK L IG+ISAIP++ G Sbjct: 246 PLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGAT 305 Query: 307 AMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAIGILAAFPV 366 AMV+ H++K G+ R +S +++A + L+ + N + L + +A G L + + Sbjct: 306 AMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIAL 365 Query: 367 FWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALEFLA 426 FW PGA+ G AA GIA+INS+GNL GFV PY++GAL T+ GS GL + FLA Sbjct: 366 FWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLA 425 Query: 427 SFLVVVFVKKH 437 L+ + +K Sbjct: 426 GVLIALGGRKR 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,154,232 Number of extensions: 43444 Number of successful extensions: 302 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 23 Length of query: 437 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 349 Effective length of database: 1,203,190 Effective search space: 419913310 Effective search space used: 419913310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)