BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0169.p (297 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [... 480 e-137 gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [... 138 5e-34 gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herba... 82 6e-17 >gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 480 bits (1236), Expect = e-137, Method: Compositional matrix adjust. Identities = 246/297 (82%), Positives = 270/297 (90%) Query: 1 MKVAVMGAGAVGCYFAGMLARAGHDVVLVGRPQHVEAFNTKGLRLEAKTFDETLPVEAST 60 MKVAVMGAGAVGCYFAGMLAR GH V LVGRPQHVEAFNT+GLRLE K FDET+ V+AST Sbjct: 1 MKVAVMGAGAVGCYFAGMLARVGHQVALVGRPQHVEAFNTRGLRLETKAFDETIAVKAST 60 Query: 61 DPAIVAGADLVLFCVKSPDTETAGAQLRPHLAPDTLVLTLQNGTDNAERLRTVIPQPVSA 120 D AI A A+LVLFCVKSPDTETAGAQL PHLAPDTLVL+LQNGTDNAERLRTV+ QPV+A Sbjct: 61 DAAIAADAELVLFCVKSPDTETAGAQLAPHLAPDTLVLSLQNGTDNAERLRTVMAQPVAA 120 Query: 121 AVVYVATEMAGPGHVKHHGRGELVIEPSARSQQAAEALIAAGVPTEISENVRGALWLKLI 180 AVVYVATEMAGPGHVKHHGRGELVIEP+A S+ AA+AL+AAGVPTEIS+NVRG LWLKLI Sbjct: 121 AVVYVATEMAGPGHVKHHGRGELVIEPAAGSRDAADALVAAGVPTEISDNVRGELWLKLI 180 Query: 181 INCAYNALSAITLLPYGKLVEGVGVLEVMDHLVSECKAVAAAEGVTLAGDVDVAIRKIIE 240 +NCAYNALSAI LPYGKLVEG G+ EV+ LV+ECKAVAAAEGVTLA DVD A+RKI++ Sbjct: 181 LNCAYNALSAIAQLPYGKLVEGPGIPEVIAQLVTECKAVAAAEGVTLADDVDSAVRKIVQ 240 Query: 241 TMPTQFSSTAQDVARGKPSEIDHLNGYIVRRGQAHGIATPVNLTVHALVKLIESKAR 297 TMP QFSSTAQD+ARGKPSEIDHLNG+IV R QAHG++ P NLTVHALVKLIESKA+ Sbjct: 241 TMPGQFSSTAQDLARGKPSEIDHLNGFIVARAQAHGLSAPANLTVHALVKLIESKAQ 297 >gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 138 bits (348), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 105/302 (34%), Positives = 153/302 (50%), Gaps = 10/302 (3%) Query: 1 MKVAVMGAGAVGCYFAGMLARAGHDVVLVG-RPQHVEAFNTKGLRLEAKTFDETLPVEAS 59 MK+A++GAGA+G FA LA AG DV L+ P +EA ++ GL L + T+ V Sbjct: 1 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG 60 Query: 60 TDPAIVAGADLVLFCVKSPDTETAGAQLRPHLA-PDTLVLTLQNGTDNAERLRTVIPQP- 117 + DL++ K T+ A A + HLA P T L++QNG NA+ + VIP+ Sbjct: 61 RADSFQDAFDLIILFTKGFHTQAAIASV-AHLAGPHTWALSVQNGLGNADLIAEVIPRER 119 Query: 118 VSAAVVYVATEMAGPGHVKHHGRGELVI-----EPSARSQQAAEALIAAGVPTEISENVR 172 + + + ++ PG V HG G++ I +PSA+ Q A AAG+P V+ Sbjct: 120 IIVGMTNLPADLRAPGVVHSHGEGQVRIWTLSGQPSAQLTQVAADFNAAGLPCSADPLVQ 179 Query: 173 GALWLKLIINCAYNALSAITLLPYGKLVEGVGVLEVMDHLVSECKAVAAAEGV-TLAGDV 231 A+W KL N A NA+ A+T L G E+ + +E AVA A G+ V Sbjct: 180 QAIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIAAETVAVAQAAGIDARLVQV 239 Query: 232 DVAIRKIIETMPTQFSSTAQDVARGKPSEIDHLNGYIVRRGQAHGIATPVNLTVHALVKL 291 + AI T S QD+ G+ +EID +NG +VR + G+ P T+H LVKL Sbjct: 240 EDAIEHAFRTHLHHKPSMLQDIEAGRRTEIDFINGAVVREAERLGLPVPTTQTLHQLVKL 299 Query: 292 IE 293 +E Sbjct: 300 ME 301 >gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 81.6 bits (200), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 77/319 (24%), Positives = 142/319 (44%), Gaps = 32/319 (10%) Query: 1 MKVAVMGAGAVGCYFAGMLARAGHDVVLVGRPQHVEAFNTKGLRLEAKTFDETLPVEAST 60 +++ + GAGA+G A LA AGH V ++ R Q ++A GL L T + Sbjct: 15 LRICIAGAGAIGGTLAVRLAAAGHQVSVLARGQTLQAIREHGLTLH--DLHGTAHARPAA 72 Query: 61 DPAIVAGA-DLVLFCVKSPDTETAGAQLRPHLAPDTLVLTLQNGTD-----------NAE 108 D G D++ C KS D Q+ P + T+V+ NG + + Sbjct: 73 DHRPSFGVQDVIFLCAKSQDMAGLLPQVAPMVGETTVVIPTNNGVPWWYFHREGGRFDGQ 132 Query: 109 RLRTVIPQ----------PVSAAVVYVATEMAGPGHVKHHGRGELVI-EPSA----RSQQ 153 ++ V P+ + +V+++ E+ G ++ +V+ EPS R + Sbjct: 133 TVKAVDPEGALAAALPLAQIIGSVLFITAEVESAGVIRSVNPHLMVLGEPSGEMSERLLR 192 Query: 154 AAEALIAAGVPTEISENVRGALWLKLIINCAYNALSAITLLPYGKLVEGVGVLEVMDHLV 213 A+ +AG+ ++ +R LW K+I N + N LS IT +L + EV+ ++ Sbjct: 193 VRAAVESAGIEARATDRIRDKLWTKIIANISTNPLSVITQGTLQQLYGLPELREVVSQIM 252 Query: 214 SECKAVAAAEGVTLAGDVD-VAIRKIIETMPTQFSSTAQDVARGKPSEIDHLNGYIVRRG 272 E VA++ G + D+D + ++ E M +S QD+ RG+P E+ + ++ Sbjct: 253 RETLLVASSHGARV--DIDPLTFLQLGEAMGAFRTSMLQDLERGRPLELAAIGDAVLEMA 310 Query: 273 QAHGIATPVNLTVHALVKL 291 + + I P+ + +L + Sbjct: 311 ERYAIPMPITRAIVSLARF 329 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 917,117 Number of extensions: 37320 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 3 Length of query: 297 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 212 Effective length of database: 1,217,395 Effective search space: 258087740 Effective search space used: 258087740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)