BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0168.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310738|ref|YP_003774830.1| transcription regulator protein... 192 2e-50 gi|300311999|ref|YP_003776091.1| GntR family transcription regul... 88 6e-19 gi|300313741|ref|YP_003777833.1| GntR family transcription regul... 77 1e-15 gi|300313534|ref|YP_003777626.1| GntR family transcription regul... 74 2e-14 gi|300311599|ref|YP_003775691.1| transcription regulator protein... 74 2e-14 >gi|300310738|ref|YP_003774830.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 234 Score = 192 bits (488), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 103/216 (47%), Positives = 132/216 (61%) Query: 30 GSLPEYVYAQLRQDIFDMRLLPGDQITETEIAAYFKVSRTPVRQALQRLQNDGLMQGYVR 89 +L E VY +L+ DIF RLLPGD TETE+A VSRTP+R AL RLQ +G ++ R Sbjct: 7 NALAEQVYHRLKDDIFSFRLLPGDNFTETEMAQRQGVSRTPLRDALFRLQREGYLEVGFR 66 Query: 90 GGWEVVPIDFKRFSDLYEMRKLIETHAVRRLCAQAGDQSVLAGLRATWCVAAEAREPDGP 149 GW+V PIDF R +LY++R ++E A+ +LC+Q + L L A W +AR+ D Sbjct: 67 RGWKVCPIDFDRLDNLYDLRIVLEMAALDKLCSQPEASAELTQLNAIWLAEPDARQTDAV 126 Query: 150 KAALLDEVFHQTLVRAAGNQEMAESFDRLTDRIRIVRRLDFLYGDCLHSTYEEHAAILDS 209 K A LDE FH LV AAGN+EM +T++IRIVRRLDFL + +TYEEH IL Sbjct: 127 KVAQLDEAFHTNLVAAAGNEEMTRVHTEVTEKIRIVRRLDFLKKKRIDATYEEHGKILQL 186 Query: 210 IAAGDAEGAMRLMGEHIDGSHAEVNQITLHRLHSAR 245 IA A A L+ HI S EV +IT+ L AR Sbjct: 187 IAKRKATEAQILLRAHITQSKLEVRKITIWMLAEAR 222 >gi|300311999|ref|YP_003776091.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 222 Score = 88.2 bits (217), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 70/212 (33%), Positives = 110/212 (51%), Gaps = 8/212 (3%) Query: 40 LRQDIFDM----RLLPGDQITETEIAAYFKVSRTPVRQALQRLQNDGLMQGYVRGGWEVV 95 LR+ I ++ +L PG + ET +A F VSRTP+R+AL +L + G+++ R G V Sbjct: 11 LRESIEELIAVSKLSPGQHLDETSLAEEFGVSRTPIREALIQLASMGIVEMRPRRGAIVA 70 Query: 96 PIDFKRFSDLYEMRKLIETHAVRRLCAQAGDQSVLAGLRATWCVAAEAREPDGPKAAL-L 154 I ++ +++E+ E RL A+ + A L A AR+ + P A L Sbjct: 71 EIGPQQLIEMFEVMAEFEA-MCGRLAARRMTPAEHAELLAAHQACQAARDANDPDAYFYL 129 Query: 155 DEVFHQTLVRAAGNQEMAESFDRLTDRIRIVRRLDFLYGDCLHSTYEEHAAILDSIAAGD 214 +E FH + + N +A+ L R+R RRL D L S+++EH A++D+I +GD Sbjct: 130 NEAFHDHIYAGSHNGFLADQARALHRRLRPYRRLQLRVRDRLKSSFDEHQAVVDAIISGD 189 Query: 215 AEGAMRLMGEH--IDGSHAEVNQITLHRLHSA 244 AE + L+ EH I G +LH+L SA Sbjct: 190 AEKTVDLLREHIMIQGQRFADLVASLHQLKSA 221 >gi|300313741|ref|YP_003777833.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 222 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 61/201 (30%), Positives = 96/201 (47%), Gaps = 5/201 (2%) Query: 34 EYVYAQLRQDIFDMRLLPGDQITETEIAAYFKVSRTPVRQALQRLQNDGLMQGYVRGGWE 93 E VY LRQ + + PG Q+ E +A VSRTPVR AL+RL DGL G Sbjct: 6 EKVYEVLRQRLVGGQYKPGTQLKEEPLARELGVSRTPVRTALRRLVEDGLATADAGQGIH 65 Query: 94 VVPIDFKRFSDLYEMRKLIETHAVRRLCAQAGDQSVLAGLRATWCVAAEAREPDG----P 149 V + +++R L+E +A L A+ GD+ +LA LR + A+A + G Sbjct: 66 VAAWSDWDIEETFQLRMLLEPYAA-SLAAERGDEKMLALLRESNEKMAQAIKSGGLHSVE 124 Query: 150 KAALLDEVFHQTLVRAAGNQEMAESFDRLTDRIRIVRRLDFLYGDCLHSTYEEHAAILDS 209 K + +FH+T++ + + + + + D IVR L + H +L + Sbjct: 125 KIQAANSIFHRTILEYSASPRLRAMLETIIDMPIIVRSFHLYEKSELEQSLHHHQDLLLA 184 Query: 210 IAAGDAEGAMRLMGEHIDGSH 230 + A DAE A ++M H+ S+ Sbjct: 185 MQARDAELARQVMQLHLRMSY 205 >gi|300313534|ref|YP_003777626.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 58/203 (28%), Positives = 99/203 (48%), Gaps = 4/203 (1%) Query: 36 VYAQLRQDIFDMRLLPGDQITETEIAAYFKVSRTPVRQALQRLQNDGLMQGYVRGGWEVV 95 + ++ + I +L PG ++ E ++A FKVSRT VR+AL L G++ R GW V+ Sbjct: 13 IAVRITEAILAKQLAPGTRLGEQQLADLFKVSRTQVREALTHLMARGIVTVSARRGWFVI 72 Query: 96 PIDFKRFSDLYEMRKLIETHAVRRLCAQAGDQSVLAGLRATWC--VAAEAREPDGPKAAL 153 + + +E R++IE +R+ + + + LR AA A G ++ L Sbjct: 73 EPTPAQAREAFEARRVIELGLLRQARLRPVGDTAIGQLREHIAREEAAIAGADVGARSYL 132 Query: 154 LDEVFHQTLVRAAGNQEMAESFDRLTDRIRIVRRLDFLYGDCLHSTYEEHAAILDSIAAG 213 L + FH L GN +AE+ LT R + L + + EH I++++ G Sbjct: 133 LGD-FHVCLAECLGNSLLAETLRDLTARTTLTAML-HQSSEQAEDSCSEHVLIVEALERG 190 Query: 214 DAEGAMRLMGEHIDGSHAEVNQI 236 D E A +LM +H+ + +NQ+ Sbjct: 191 DLEQAEQLMHDHLQHVESGLNQV 213 >gi|300311599|ref|YP_003775691.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 69/249 (27%), Positives = 112/249 (44%), Gaps = 28/249 (11%) Query: 19 DLEGEDALVRNGSLPEYVYAQLRQDIFDMRLLPGDQITETEIAAYFKVSRTPVRQALQRL 78 D EGE +L E+V+ +++ I + PG +I+E E+A + +SR P+R+A+ RL Sbjct: 11 DREGE-------TLSEHVFRKIQSAIVQGEIAPGSKISEPELARTYGISRGPLREAIHRL 63 Query: 79 QNDGLMQGYVRGGWEVVPIDFKRFSDLYEMRKLIETHAVRRLCAQAGDQSVLAGLRATWC 138 + L+ G VV + + +L+++R+ +E A RL AQ ++ L LR Sbjct: 64 EGQNLLVRVPHVGARVVSLTLEGLVELFQIRESLEGMAC-RLAAQRMSRAELDELRRVL- 121 Query: 139 VAAEAREPD-------GPKAALLDEVFHQTLVRAAGNQEMAESFDRLTDRIRIVRRLDFL 191 + E D G D FH +++A+GNQ + + ++ R Sbjct: 122 ---DTHEKDEAFQAGRGYYQQEGDYDFHYKIIQASGNQILTRIL--CGELYQLARMYRIQ 176 Query: 192 YGDCLH---STYEEHAAILDSIAAGDAEGAMRLMGEHIDGSHAEVNQITLHRLHSARATA 248 Y H + EH ILD++A GD E A LM HI S + H++ A+A Sbjct: 177 YSATPHRPRQAFAEHHRILDALADGDGELADLLMRRHIGASRRNIE----HQIAQAQAGG 232 Query: 249 TTKPPAYVS 257 K S Sbjct: 233 AGKRAGQAS 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 772,885 Number of extensions: 31305 Number of successful extensions: 142 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 133 Number of HSP's successfully gapped: 11 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)