BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0159.p (339 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi... 661 0.0 gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum... 135 5e-33 gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro... 103 3e-23 >gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae SmR1] Length = 340 Score = 661 bits (1706), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/340 (96%), Positives = 331/340 (97%), Gaps = 1/340 (0%) Query: 1 MTSFKQLAAVAAIGLVFGNAYAAGDAGVEPTTQSFLQALEAGGGKPLEQLSPQDARAVLV 60 MTSFKQLAAVAAIGLVFGNAYAAGDAGVEPTTQ+FLQALEAGGGKPLEQLSP DARAVLV Sbjct: 1 MTSFKQLAAVAAIGLVFGNAYAAGDAGVEPTTQAFLQALEAGGGKPLEQLSPADARAVLV 60 Query: 61 GAQASVKLELPKADVSEKTITADGQQIKLTIVRPAG-VTKTLPAFMFFHGGGWVLGDFPT 119 GAQASVKL+LPKADVSEKTI+ADGQQIKLTIVRPAG KTLPAFMFFHGGGWVLGDFPT Sbjct: 61 GAQASVKLQLPKADVSEKTISADGQQIKLTIVRPAGSAKKTLPAFMFFHGGGWVLGDFPT 120 Query: 120 HERLVRDLVANSGAVAVFVNYTPSPEAGYGVAINQAYAATKWVAEHGKEIKVDGKRLAVA 179 HERLVRDLVANSGAVAVFVNYTPSPEA YGVAINQAYAATKWVAEHGKEI VDGKRLAVA Sbjct: 121 HERLVRDLVANSGAVAVFVNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVA 180 Query: 180 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWF 239 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWF Sbjct: 181 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWF 240 Query: 240 WDSYTTDAAKRKEITASPLNATPAQLAGLPPTLIQTAENDVLRDEGEAYGRKLQAAGVAV 299 WDSYTTDAAKRKEITASPLNATPAQL+GLPPTLIQTAE DVLRDEGE YGRKL AAGVAV Sbjct: 241 WDSYTTDAAKRKEITASPLNATPAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAV 300 Query: 300 TSVRYNGMIHDFGLLNVLAKVPAVQTAMRQAGEELKFRLK 339 TSVRYNGMIHDFGLLNVLAKVPAVQTAMRQAGEELKFRLK Sbjct: 301 TSVRYNGMIHDFGLLNVLAKVPAVQTAMRQAGEELKFRLK 340 >gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum seropedicae SmR1] Length = 317 Score = 135 bits (340), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 95/295 (32%), Positives = 147/295 (49%), Gaps = 17/295 (5%) Query: 28 VEPTTQSFLQALEAG----GGKPLEQLSPQDARAVLVGAQASVKLELPKADVSEKTI--- 80 + P +FL +EAG +P+ + S ARA A + L+LP D++E+ Sbjct: 3 LHPDLDAFLALVEAGIQSGRQQPMHRKSVHQARADYEAA--APMLDLPPDDLAERRALEI 60 Query: 81 -TADGQQI--KLTIVRPAGVTKTLPAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF 137 T DG +I +L P P ++FHGGG+ +G +H+ L L A + + Sbjct: 61 PTRDGARIGARLYAPAPLDAAAPQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLA 120 Query: 138 VNYTPSPEAGYGVAINQAYAATKWVAEHGKEIKVDGKRLAVAGNSVGGN-MAAVVALMAK 196 V+Y +PE + A A A W+ +HG + + RLAV G+S GG AA+ + Sbjct: 121 VDYRLAPEHRFPTAFEDAADAYDWLLQHGPALGLATNRLAVGGDSAGGTLAAALALRARQ 180 Query: 197 DKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITAS 256 P L +VLL+P T A+ + S+ +FA GY L ++W + +R++ + Sbjct: 181 AARAPCL--QVLLYPCTSAHQDGDSHRRFATGYLLEGETLQWMFGHTLNGDHERQDWRFA 238 Query: 257 PLNATPAQLAGLPPTLIQTAENDVLRDEGEAYGRKLQAAGVAVTSVRYNGMIHDF 311 PL A A ++G P L+ ++D L DEG AY KL+ AGV V + GM+HDF Sbjct: 239 PLQA--ADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDF 291 >gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 103 bits (256), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 73/209 (34%), Positives = 102/209 (48%), Gaps = 5/209 (2%) Query: 102 PAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF-VNYTPSPEAGYGVAINQAYAATK 160 P M+FHGGG+V G + R +A G +AV V Y +P + A A+AA Sbjct: 84 PLVMYFHGGGFVSGSLEDADTPAR-FIAEHGQLAVLSVGYALAPAKPFPAAPEDAFAAAT 142 Query: 161 WVAEHGKEIKVDGKRLAVAGNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETA 220 W ++ E+KVD R+ VAG+ GG +AA + LM +D+ G L ++VL+ P+ D + Sbjct: 143 WACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCGKPLAAQVLISPMLDPSMRLL 202 Query: 221 SYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITASPLNATPAQLAGLPPTLIQTAENDV 280 LT + Y +R A+PL + +LAGLPP I TAE DV Sbjct: 203 GDAARLQSD-LTAETCAASYRQYLPQTMQRLHPYAAPLEVS--RLAGLPPAFIATAERDV 259 Query: 281 LRDEGEAYGRKLQAAGVAVTSVRYNGMIH 309 L E E Y L AGV R+ G+ H Sbjct: 260 LCPEAEKYASSLIRAGVPTQVSRFAGITH 288 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,139,745 Number of extensions: 50741 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 3 Length of query: 339 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 253 Effective length of database: 1,212,660 Effective search space: 306802980 Effective search space used: 306802980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)