BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0154.p (608 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspi... 852 0.0 gi|300311184|ref|YP_003775276.1| acetyltransferase [Herbaspirill... 312 5e-86 gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE prot... 297 1e-81 gi|300314038|ref|YP_003778130.1| hypothetical protein Hsero_4761... 139 6e-34 gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspi... 62 1e-10 >gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 454 Score = 852 bits (2202), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/426 (96%), Positives = 424/426 (99%), Gaps = 1/426 (0%) Query: 1 MSDTPFTHSQDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDK 60 MSDTPFTHSQDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDK Sbjct: 1 MSDTPFTHSQDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDK 60 Query: 61 GIGVTVFLGEGRQVRRGNASTSDFSQQALKETVEAAYNIARFTAIDDCAGLPDAEMLEMS 120 GIGVTVFLGEGRQVRRGNASTSDFSQQALKETVEAAYNIARFT+IDDCAGLPDAEMLEM+ Sbjct: 61 GIGVTVFLGEGRQVRRGNASTSDFSQQALKETVEAAYNIARFTSIDDCAGLPDAEMLEMA 120 Query: 121 PRDLKLFSPWNISAEEAVALAQRCETAALETDPRITNSEGAGVYVQHSHFIAANSRGFMG 180 PRDLKLFSPWNISAEEAVALAQRCETAALETDPRI+NSEGAGVYVQHSHFIAANSRGFMG Sbjct: 121 PRDLKLFSPWNISAEEAVALAQRCETAALETDPRISNSEGAGVYVQHSHFIAANSRGFMG 180 Query: 181 GYPFSRHTISVAPIAGKGAGMQRDDWYS-KRDPKKLAKPEAIGRYAAERALARLNARKLT 239 GYPFSRHTISVAPIAGKGA MQRDDWYS KRDPKKLA+PEAIGRYAAERALARLNARKL+ Sbjct: 181 GYPFSRHTISVAPIAGKGASMQRDDWYSSKRDPKKLAQPEAIGRYAAERALARLNARKLS 240 Query: 240 TRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVVGG 299 TRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHV+GG Sbjct: 241 TRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVIGG 300 Query: 300 VGSAPFDEEGVRTVKREVIKDGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLRSDLTTK 359 VGSAPFDEEGVRT+KREV+++GVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLRS+LTT+ Sbjct: 301 VGSAPFDEEGVRTLKREVVRNGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLRSELTTR 360 Query: 360 ADTFKGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAGNMK 419 ADTFKGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAGNMK Sbjct: 361 ADTFKGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAGNMK 420 Query: 420 DMLRQI 425 DMLRQI Sbjct: 421 DMLRQI 426 >gi|300311184|ref|YP_003775276.1| acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 163 Score = 312 bits (799), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 146/163 (89%), Positives = 154/163 (94%) Query: 446 MDDIHIRRGYLPGAIGRVAELHAAYYTENWSFGVYFEARIATELAAFMSRYDDSRDGFWT 505 MDDIHIRRGYLPGAIGRVAELHAAYYTENWSFGVYFEARIATELAAFMSRYDD+RDGFWT Sbjct: 1 MDDIHIRRGYLPGAIGRVAELHAAYYTENWSFGVYFEARIATELAAFMSRYDDARDGFWT 60 Query: 506 ATVHGRVEGSLTIDGQHAQQQGAWLRWFISSEKLRGRGVGDALLTEAIRFCAECDFPSIY 565 ATVHGR+EG+LTIDGQHAQ+QGAWLRWFI+SEKLRGRGVGDALL+EA+R CAE D SIY Sbjct: 61 ATVHGRIEGALTIDGQHAQEQGAWLRWFIASEKLRGRGVGDALLSEAVRCCAERDIASIY 120 Query: 566 LWTFEGLHAAHNLYQRHGFVLAEERIGSHWGVKLNEQRYELLL 608 LWTFEGLHAA LY+RHGFV EERIGSHWGVKL EQRYELLL Sbjct: 121 LWTFEGLHAARTLYERHGFVRVEERIGSHWGVKLKEQRYELLL 163 >gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 297 bits (761), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 163/417 (39%), Positives = 236/417 (56%), Gaps = 5/417 (1%) Query: 10 QDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDKGIGVTVFLG 69 Q + +AQ L AR GA+ AAV I E LS +V+ GK + + +++FLG Sbjct: 17 QQHFETIAQQTLEVARQHGATAAAVQIEEVESLSASVQGGKPNERALRQSAQLRLSLFLG 76 Query: 70 EGRQVRRGNASTSDFSQQALKETVEAAYNIARFTAIDDCAGLPDAEMLEMSPRDLKLFSP 129 + R + S+SD SQQ + V + A A D AGLP DL+L Sbjct: 77 Q----REASVSSSDLSQQGVAALVARGMDSAAIAAEDQHAGLPGLSAYPSDDVDLELDHA 132 Query: 130 WNISAEEAVALAQRCETAALETDPRITNSEGAGVYVQHSHFIAANSRGFMGGYPFSRHTI 189 W + +AVAL R E AAL + E A ++ + + ANS GF+ +RH++ Sbjct: 133 WQLGLRDAVALVARIERAALAVSSAVPQIEQAVLFSSRTRRVLANSEGFLRSSASTRHSL 192 Query: 190 SVAPIAGKGAGMQRDDWY-SKRDPKKLAKPEAIGRYAAERALARLNARKLTTRKCPVLFE 248 S +A + + + W+ ++RDP +L PE++G A RALARL AR++ T+ CPVLFE Sbjct: 193 SCTAVAIRDGAKEIESWWDARRDPAQLPSPESLGACAGRRALARLGARQVATQTCPVLFE 252 Query: 249 APLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVVGGVGSAPFDEE 308 AA LL FVQA+S LY S+FL D+LGQ+VF H+ I +DP + GG+GS +D Sbjct: 253 PQAAASLLNEFVQAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGN 312 Query: 309 GVRTVKREVIKDGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLRSDLTTKADTFKGMLK 368 G R +I G +QGY+L Y AR+LG+ + G N+ + S + + + M++ Sbjct: 313 GAALRPRTLIAGGRLQGYYLGAYGARRLGLPLAEHGAGPSNVMVSSTTSAASGDLEAMIR 372 Query: 369 KMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAGNMKDMLRQI 425 +MGTGL+++ L GQGVN +TGD+SR SG+WVENG I YPV IT+A N+++M I Sbjct: 373 RMGTGLVISGLNGQGVNLMTGDFSRACSGFWVENGQIAYPVAGITVASNLREMFAGI 429 >gi|300314038|ref|YP_003778130.1| hypothetical protein Hsero_4761 [Herbaspirillum seropedicae SmR1] Length = 326 Score = 139 bits (350), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 75/154 (48%), Positives = 90/154 (58%), Gaps = 2/154 (1%) Query: 451 IRRGYLPGAIGRVAELHAAYYTENWSFGVYFEARIATELAAFMSRYDDSRDGFWTATVHG 510 + +GY PG IG +A LHA YY E FGVYFE ++A+EL AF+ WT HG Sbjct: 168 LAQGYQPGCIGDIASLHARYYAEAAGFGVYFEHKVASELGAFVQSLPAPGKAIWTHQEHG 227 Query: 511 RVEGSLTIDGQHAQQQGAWLRWFISSEKLRGRGVGDALLTEAIRFCAECDFPSIYLWTFE 570 R S+ IDG A A LRWFI + LRGRGVG LL +A+ F YLWTF+ Sbjct: 228 RTLASIVIDGDVASGL-AHLRWFIVDDSLRGRGVGRRLLEQAMALVDAHHF-DCYLWTFQ 285 Query: 571 GLHAAHNLYQRHGFVLAEERIGSHWGVKLNEQRY 604 GL AA +LY GF LAEE G WG ++ EQRY Sbjct: 286 GLDAARHLYLEAGFALAEESPGEQWGSRVVEQRY 319 >gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 486 Score = 62.0 bits (149), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 44/154 (28%), Positives = 73/154 (47%), Gaps = 7/154 (4%) Query: 279 LGQQVFPEHIQILEDPHVVGGVGSAPFDEEGVRTVKREVIKDGVVQGYFLSTYSARKLGM 338 +G++V + + +++D + GS D+EG T +I+DG+++GY T +AR + M Sbjct: 290 IGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKM 349 Query: 339 QTTGNSGGSHNLTLRSDLTTKADTFKG------MLKKMGTGLLVTELMGQGVNYVTGDYS 392 TGN+ L T G +L + GL G V+ G + Sbjct: 350 DVTGNARRESFAHLPMPRMTNTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFV 409 Query: 393 RGAS-GYWVENGVIQYPVEEITIAGNMKDMLRQI 425 AS Y +E+G I YPV+ T+ GN D+L ++ Sbjct: 410 FSASEAYMIEDGKITYPVKGATLIGNGPDVLNRV 443 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,006,789 Number of extensions: 89470 Number of successful extensions: 267 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 259 Number of HSP's successfully gapped: 6 Length of query: 608 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 517 Effective length of database: 1,188,985 Effective search space: 614705245 Effective search space used: 614705245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)