BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0153.p (532 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 1004 0.0 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 139 6e-34 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 135 8e-33 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 118 2e-27 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 113 4e-26 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 1004 bits (2596), Expect = 0.0, Method: Compositional matrix adjust. Identities = 511/532 (96%), Positives = 526/532 (98%) Query: 1 MALHTDAAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFDLYAW 60 MALHTD+AHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGF+LYAW Sbjct: 1 MALHTDSAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAW 60 Query: 61 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIVVFVGASALCGMANSMLFLVLARGLQG 120 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASI+VFVGASALCGMANSMLFLVLARGLQG Sbjct: 61 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG 120 Query: 121 IGGGMLVGTCFASIADLFPDPRVRLRWQIILSASFGIATAVGPSLGGFLTQGLGWRWVFY 180 IGGGMLVGTCFAS+ADLFPDPRVRLRWQIILS SFGIATAVGPSLGGFLTQGLGWRWVFY Sbjct: 121 IGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFY 180 Query: 181 CNLPIGVLSLYFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGITG 240 CNLPIGV+SL+FVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRG+T Sbjct: 181 CNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTS 240 Query: 241 GMMVLLAVSVAAFYALWRWEQRAAQPILPFEMMRDKALASLFVLSVLAGFAMFSLLMYAP 300 GM+VLLAVSVAAFYALW+WEQRAAQPILPFEM+RD+ALASLF+LSVLAGFAMFS+LMYAP Sbjct: 241 GMLVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAP 300 Query: 301 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGV 360 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLG+ Sbjct: 301 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGI 360 Query: 361 VLCDRTTGNGFLLTVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML 420 VLCDR TG GFLL VMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML Sbjct: 361 VLCDRVTGKGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML 420 Query: 421 GTAITGTLVSQMYGAGVQKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGS 480 GTAITGTLVSQMYGAGV+KALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGS Sbjct: 421 GTAITGTLVSQMYGAGVEKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGS 480 Query: 481 ALLEAAREALVGAIHMGIAIAIVAALAGLWMVRRVPPIRFDQDKKVEPVMSE 532 ALLEAAREALVGAIHMGIAIAIVAAL GLWMVRRVPPIRFDQDKKVEPVMSE Sbjct: 481 ALLEAAREALVGAIHMGIAIAIVAALVGLWMVRRVPPIRFDQDKKVEPVMSE 532 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 139 bits (350), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 126/437 (28%), Positives = 202/437 (46%), Gaps = 15/437 (3%) Query: 22 LATLGICFVIMLVALDQTIVGTALPTIVAELKG-FDLYAWVATAYLLTSVITVPIFGRLG 80 L L I FV+ + LD T+V L I L W+ Y LT + + G L Sbjct: 32 LVALAIGFVMAM--LDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALA 89 Query: 81 DYFGRKPFVIASIVVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASIADLFPD 140 D FG + + + +FV AS LCG A + FL++AR LQG+G + + + + ++ D Sbjct: 90 DRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYED 149 Query: 141 PRVRLRWQIILSASFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVLSLYFVWRFVPHI 200 RVR R + SA+ G+A AVGP +GG L LGWR VF N+P+G++ L + +P + Sbjct: 150 DRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQV 209 Query: 201 RHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGITGGMMVL-LAVSVAAFYALWRW 259 A + G ++ L L ++ P G T ++ LAV+++A AL R Sbjct: 210 GGHARALNLSSHLAGVVM----LAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRH 265 Query: 260 EQRAAQPILPFEMMRDKALASLFVLSVLAGFAMFSLLMYAPLLFQGGFGLSPREAGLLIT 319 E R P+LP + A+ + L F ++ L Y L Q G G + GL + Sbjct: 266 ELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 320 PLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGVVLCDRTTGNGFLLTVMLAG 379 P++A I ++++GR+ P + + LG ++ + T L V + Sbjct: 326 PMMAVIFFGNLMSGRMSAHW-GPRVPLLLGLSVGTVFTALAMGFSPATPYWLLALVCASA 384 Query: 380 GFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLVSQMYGAGVQK 439 G+ +P +T Q A + H A A L + R IG ++G A+ G ++ + G ++ Sbjct: 385 NLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVTGWNLRN 444 Query: 440 AL------EGDHAAQWL 450 L G +A WL Sbjct: 445 PLAFGVMAAGYASALWL 461 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 135 bits (340), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 132/451 (29%), Positives = 204/451 (45%), Gaps = 23/451 (5%) Query: 1 MALHTDAAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELK-GFDLYA 59 M L +D H + + LP ++ A L + + D TI+ ALP I L+ + Sbjct: 10 MELFSD--HPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSI 67 Query: 60 WVATAYLLTSVITVPIFGRLGDYFGRKPFVIASIVVFVGASALCGMANSMLFLVLARGLQ 119 WV+ AYLL + +T+ F L G + A + VF AS C ++ S+ L+L R LQ Sbjct: 68 WVSNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQ 127 Query: 120 GIGGGMLVGTCFASIADLFPDPRVRLRWQII-LSASF-GIATAVGPSLGGFLTQGLGWRW 177 G+GG ++ A +FP+ RL +I+ L+A +TAVGP LGG L LGW W Sbjct: 128 GLGGAAMLSIAPAIHRTIFPN---RLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEW 184 Query: 178 VFYCNLPIGVLSLYFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPK-- 235 +F N+P+GVL+++ W VP R A D GAL +L++G+L + + + Sbjct: 185 IFAINVPLGVLAVWLAWNAVPDTRKPAQAA---FDLAGALWSALAMGALIMAADTCARFA 241 Query: 236 -----RGITGGMMVLLAVSVAAFYALWRWEQRAAQPILPFEMMRDKALASLFVLSVLAGF 290 G ++ A A R ++RAA P+LP ++ + SL L+ LA F Sbjct: 242 EPGQGSGAAFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRF-SLAALTSLASF 300 Query: 291 AMFSLLMYA-PLLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLG 349 + A P LFQ +G + E+ L TP I + + GR+ R L Sbjct: 301 VGQGIAFVALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGL 360 Query: 350 FALIAASCLGVVLCDRTTGNGFLLTVMLAG-GFGLGLVLPNLTVFAQQAAAREHLGIATA 408 L +L D+ ++ G GFG N + +RE G A+ Sbjct: 361 ALFALGLVLLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGN--VSRERSGNASG 418 Query: 409 LLQSLRMIGGMLGTAITGTLVSQMYGAGVQK 439 +L +R G LG A+ G +++ A V + Sbjct: 419 VLAIMRTFGQCLGAALLGVILAVRTAAAVAQ 449 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 118 bits (295), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 124/454 (27%), Positives = 209/454 (46%), Gaps = 27/454 (5%) Query: 36 LDQTIVGTALPTIVAELKGFDLYAWVA-TAYLLTSVITVPIFGRLGDYFGRKPFVIASIV 94 +D T++ T+LP + ++ + +A T+YL++ + +PI G + D FG ++I Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 95 VFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASIADLFPDPR-VRLRWQIILSA 153 VF+ S LCG+++++ V AR LQGIGG M+V I V+ + + A Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 154 SFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVLSLYFVWRFVPHIRHMQHAGKMRLDW 213 FG +GP LGGF+T WRW+F+ N+PI +L LY RF+P ++ Q A LD Sbjct: 121 LFG--PVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVA---PLDL 175 Query: 214 PGALLISLSLGSLQLLVELLPKRGITGGMMVLLAVSVAAFYALWRWE-QRAAQPILPFEM 272 G LL +L L + + + ++G + + ++ F A + W +RA+ P++ + Sbjct: 176 KGFLLSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLAL 235 Query: 273 MRDKAL-ASLFVLSVL-AGFAMFSLLMYAPLLFQGGFGLSPREAGLLITPLVACITVASI 330 ++ L A +F S G L+ PL+ Q GFG +P +G+L A Sbjct: 236 LKIPTLRAGVFGGSFYRVGIGAIPFLL--PLMLQLGFGYTPFHSGMLTCASAAGAMFMKT 293 Query: 331 VNGRIITRIPNPNLMMNLGFALIAASCLGV-VLCDRTTGNGFLLTV--MLAGGFGLGLVL 387 + R + R +++ G A LG+ + T +LL V +L GG L Sbjct: 294 IAARFLRRYGFRRVLLVNG----ALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQF 349 Query: 388 PNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLVSQMYGAGVQKALEGDHAA 447 +L + + L AT++ + + +G + G + + ++G HA Sbjct: 350 TSLNAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAG------FALQMSNEIQG-HAT 402 Query: 448 QWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGSA 481 +DF P LV + + L L Q HD A Sbjct: 403 LVAQDFW-PAFLVVGAVAACALLMLRQLPHDAGA 435 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 113 bits (283), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 115/402 (28%), Positives = 180/402 (44%), Gaps = 21/402 (5%) Query: 36 LDQTIVGTALPTIVAEL-KGFDLYAWVATAYLLTSVITVPIFGRLGDYFGRKPFVIASIV 94 LD TI ++ TI L +V T+Y + ITVP+ G L + FG I S+V Sbjct: 47 LDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMV 106 Query: 95 VFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASIADLFPDPRVRLRWQIILSAS 154 +F SALCGMANS+ L+ R LQG+ GG L+ + +FP + + + Sbjct: 107 MFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTT 166 Query: 155 FGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVLSLYFVWRFVPHIRHMQHAGKMRLDWP 214 +A GP LGG L W ++F+ N+P+ ++ Y WR + R+ K+ +D Sbjct: 167 L-VAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLK--RYETKMSKLPIDRV 223 Query: 215 GALLISLSLGSLQLLV-ELLPKRGITGGMMVLLAVSVAAFY-ALWRWEQRAAQPILPFEM 272 G +L+ + + +LQL++ E K +VLLAV+ F+ A WE PI+ + Sbjct: 224 GLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRV 283 Query: 273 MRDKALASLFVLSVLAGFAMFSLLMYAPLLFQGGFGLSPREAGLL--ITPLVACITVASI 330 R + + LA A F ++ PL QG G + +G + +T ++A +T + Sbjct: 284 FRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMV 343 Query: 331 VNGRIITRIPNPNLMMNLGFALIAASCLGVVLCDR---TTGNGFLLT--VMLAGGFGLGL 385 ++ T I L+ I + LG + R TT F + G GL L Sbjct: 344 --AKMSTTIDARKLV------CIGVTWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPL 395 Query: 386 VLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGT 427 LT A + E A L+ R + G + T+I T Sbjct: 396 FFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNT 437 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,538,537 Number of extensions: 61027 Number of successful extensions: 436 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 412 Number of HSP's successfully gapped: 15 Length of query: 532 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 442 Effective length of database: 1,193,720 Effective search space: 527624240 Effective search space used: 527624240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)