BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0028.0135.p
         (219 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba...   400   e-113
gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herba...    62   3e-11

>gi|300311201|ref|YP_003775293.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 219

 Score =  400 bits (1027), Expect = e-113,   Method: Compositional matrix adjust.
 Identities = 202/219 (92%), Positives = 211/219 (96%)

Query: 1   MAYQTWFAFFLAACVIAISPGSGAVLSMSHGLNYGLRRTTATILGLEIALVVVLIIAGAG 60
           M YQTWFAFF AACVIAISPGSGAVLSMSHGLNYG+RRT+ATILGLEIALVV+LIIAGAG
Sbjct: 1   MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60

Query: 61  VGSLLVASETAFNVVKIAGALYLIYLGFSQWRARVPSEQEGEALDNANAVARAQRGQHWW 120
           VGSLLVASETAFNV+K+AGALYLIYLGFSQWRARVPSE++GEALD ANA ARA   Q WW
Sbjct: 61  VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120

Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHERPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180
           QRCMTGFLTNATNPKGIVFMVAVLPQFIDH+RPLWLQLLVLAVTMVAVDLVVMHGYAFAA
Sbjct: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180

Query: 181 SRLQRFFRSQKAIKAQNRFFGGVLMLVGAGLFFFKRTQH 219
           SRLQRFFRSQKAI+AQNRFFGGVLMLVGAGLFFFKRTQH
Sbjct: 181 SRLQRFFRSQKAIRAQNRFFGGVLMLVGAGLFFFKRTQH 219


>gi|300312440|ref|YP_003776532.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 208

 Score = 62.4 bits (150), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 57/195 (29%), Positives = 88/195 (45%), Gaps = 9/195 (4%)

Query: 9   FFLAACVIAISPGSGAVLSMSHGLNYGLRRTTATILGLEIALVVVLIIAGAGVGSLLVAS 68
           F LAA  + ++PG   +  ++ G+  G +       G    LV   ++A  G  +L+ A 
Sbjct: 7   FLLAAMTLTLAPGPDNLYVLTRGIAQGRKAGLVAAAGFCSGLVFHTLLAVLGFAALIKAY 66

Query: 69  ETAFNVVKIAGALYLIYLGFSQWRARVPSEQEGEALDNANAVARAQRGQHWWQRCMTGFL 128
             A++ ++ AGA YL YLG    R+   S     A D   A A   R + +WQ      L
Sbjct: 67  PPAYHALQYAGAAYLAYLGVRTLRSA--SAGLALAADGQGAAAVPLR-RIYWQSV----L 119

Query: 129 TNATNPKGIVFMVAVLPQFIDHER-PLWLQLLVLAVTMVAVDLVVMHGYAFAASRLQRFF 187
            N  NPK  +F +A LPQF+  E     LQ+LVLA+  +     +    A  +  +  +F
Sbjct: 120 ANMLNPKVTLFFIAFLPQFVQREAGHEALQMLVLALVFIVQAFAIFSAIALFSGAVGAYF 179

Query: 188 RSQKAIKAQ-NRFFG 201
           R + +     NR  G
Sbjct: 180 RRRASASVHLNRLAG 194


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.329    0.139    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 585,681
Number of extensions: 19599
Number of successful extensions: 97
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 94
Number of HSP's successfully gapped: 4
Length of query: 219
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 137
Effective length of database: 1,231,600
Effective search space: 168729200
Effective search space used: 168729200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 128 (53.9 bits)