BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0116.p (667 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthet... 1288 0.0 gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) syntheta... 112 9e-26 >gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthetase [Herbaspirillum seropedicae SmR1] Length = 685 Score = 1288 bits (3334), Expect = 0.0, Method: Compositional matrix adjust. Identities = 620/659 (94%), Positives = 641/659 (97%) Query: 1 MPSPFFNLYNHGFARVAVAVPDCKVADPGFNAERTIALAEQAAARGAALVSFPELGLSAY 60 M SPFFNLY+HGFARVAVAVPDCKVADPGFNA+RTIALAEQAAARGA LVSFPELGLSAY Sbjct: 1 MQSPFFNLYSHGFARVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAY 60 Query: 61 SCEDLFQQRALLEASLQALQSVLQASLRIAPALIVGLPLKVDHQLYNCAVVVHAGRILGV 120 SCEDLFQQRALL+ASLQALQSVL+AS+RI ALIVGLPLKVDHQLYNCAVV+H GRILGV Sbjct: 61 SCEDLFQQRALLDASLQALQSVLEASMRIPAALIVGLPLKVDHQLYNCAVVLHHGRILGV 120 Query: 121 VPKSYLPNYSEFYEARQFSSADCAVTRRTRLLGQDVDFGSHLLFEIGNIPDFRFHIEICE 180 VPKSYLPNYSEFYEARQFSSADCAVTR LLGQ+V FGSHLLFEI NIPDFRFHIEICE Sbjct: 121 VPKSYLPNYSEFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICE 180 Query: 181 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE 240 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE Sbjct: 181 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE 240 Query: 241 STTDLAWDGQALICENGELLAESERFAEGGHVIYADIDLERLSRERFHQTTFGQSVRRHA 300 STTDLAWDGQALICENGELLAESERFA+GGHVIYAD+DLERLSRERFHQTTFGQSVRRHA Sbjct: 241 STTDLAWDGQALICENGELLAESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHA 300 Query: 301 EEVRRFEVVRFDVQVPQDQDLPLQRRVARFPYVPANAEQRELRCREVYNIQVQALVQRLS 360 +EVRRFEVV F+VQ+PQ Q LPLQRRVARFPYVPANAEQRELRCREVY+IQVQALVQRLS Sbjct: 301 DEVRRFEVVGFEVQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLS 360 Query: 361 SSNIKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRANILAYTMPGFATSSRTLVQAHQLM 420 S+ +KKVVIGVSGGLDSTHALLVCAKAMDKLGLPR+NILAYTMPGFATSSRTLVQAHQLM Sbjct: 361 SAGLKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLM 420 Query: 421 AQVGCSAQEIDIRPSCEQMLKDLDHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI 480 AQVGCSAQEIDIRPSCEQMLKDL HPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI Sbjct: 421 AQVGCSAQEIDIRPSCEQMLKDLGHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI 480 Query: 481 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHLVRWVAESGSLELKQPEVLIH 540 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITH+VRWVAESGSLELKQPEVLIH Sbjct: 481 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHMVRWVAESGSLELKQPEVLIH 540 Query: 541 ILETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAWQD 600 +LETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAW D Sbjct: 541 VLETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAWHD 600 Query: 601 RSIGHWPDGLHGARNQYTLPQIKKNLGIFVYRFFKTSQFKRSCVPNAPKVGSGGSLSPR 659 R +GHWPDGLHGA N+Y+LPQIKKNLGIFVYRFFKTSQFKRSCVPNAPKVGSGGSLSPR Sbjct: 601 RGVGHWPDGLHGAHNEYSLPQIKKNLGIFVYRFFKTSQFKRSCVPNAPKVGSGGSLSPR 659 >gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) synthetase [Herbaspirillum seropedicae SmR1] Length = 565 Score = 112 bits (280), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 145/592 (24%), Positives = 236/592 (39%), Gaps = 104/592 (17%) Query: 15 RVAVAVPDCKVADPGFNAERTIALAEQAAARGAALVSFPELGLSAYSCEDLFQQRALLEA 74 R+A+A + + D N + ++AA GA LV FPEL + Y DL ++ L+ Sbjct: 3 RIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFLDR 62 Query: 75 SLQALQSVLQASLRIAPAL--IVGLPLK---VDHQLYNCAVVVHAGRILGVVPKSYLPNY 129 + LQ V+ A+ R+ P L ++G P + L+N + V GRI+ K LP Y Sbjct: 63 AEATLQDVIDATRRL-PGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPVY 121 Query: 130 SEFYEARQFSSADCAVTRRTRLLGQDVDFGSHLLFEIGNIPDFRFHIEICEDVW------ 183 F + R F + + + R ICED W Sbjct: 122 GVFDDGRHFEAGPETAC-------------------VLPLKGMRVGFLICEDGWNDDLRS 162 Query: 184 VPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGESTT 243 + P A A A ++V+++AS VGK RH L R A LY + G+ Sbjct: 163 YRVNPFEKLAQADAQLVVSINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQD-- 220 Query: 244 DLAWDGQALICENGELLA-ESERFAEGGHVIYADIDLERLSRERFHQTTFGQSVRRHAEE 302 L +DG + + LA E+ R A E F F E Sbjct: 221 QLVYDGGSFAMSPLQGLAFEAARLA-----------------EDFQVLAF---------E 254 Query: 303 VRRFEVVRFD-VQVPQDQDLPLQRRVARFPYVPANAEQRELRCREVYNIQVQALVQRLSS 361 RF +R D V PQ D + E R++ + L Sbjct: 255 GGRF--LRSDGVGAPQCIDQTMA--------------TPEFHRRQI----LLGLRDYARR 294 Query: 362 SNIKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRANILAYTMPGFATSSRTLVQAHQLMA 421 KVV+G SGG+DS + + A+A+ N++A TMP +S+ ++ + L Sbjct: 295 CGFGKVVVGSSGGIDSAVTIALAAEALGA-----QNVVAVTMPSVFSSAGSVSDSETLCQ 349 Query: 422 QVGCSAQEIDIRPSCEQMLKDLDHPYSR--GEPVYDITFENVQAGERTNHLFRLANHHGA 479 +G + R ++++ + ++ G P+ + EN+QA R L +N + A Sbjct: 350 ALGVTL----FRHPIADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYDA 405 Query: 480 IVIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHLVRWVAESGSLELKQPEVLI 539 +++ TG+ SE+++G+CT GD + + KT + L R + E+ ++ Sbjct: 406 LLLTTGNKSEISVGYCTL-YGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAIID 464 Query: 540 HILETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVA 591 E++P+ QR D + PYE+ D L + + PA A Sbjct: 465 KAPSAELAPD-----------QRDTDSLPPYEVLDEILKWHIEGSRLPADEA 505 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,983,755 Number of extensions: 79422 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 3 Length of query: 667 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 575 Effective length of database: 1,184,250 Effective search space: 680943750 Effective search space used: 680943750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)