BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0115.p (113 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311220|ref|YP_003775312.1| nitrogen regulatory P-II protei... 152 7e-39 gi|300309430|ref|YP_003773522.1| nitrogen regulatory PII-like pr... 144 2e-36 >gi|300311220|ref|YP_003775312.1| nitrogen regulatory P-II protein [Herbaspirillum seropedicae SmR1] Length = 112 Score = 152 bits (384), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 91/104 (87%), Positives = 93/104 (89%) Query: 1 MKQVTAIIKPFKLDEVRESLAEVGVTGLTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK 60 MKQVTAIIKPFKLDEVRESLAEVGVTGLTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK Sbjct: 1 MKQVTAIIKPFKLDEVRESLAEVGVTGLTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK 60 Query: 61 IEVVVDDKVVEQAVDAIIKAARTGKIGDXRRKALRVWQAVAARC 104 IEVVVDDKVVEQAVDAIIKAARTGKIGD + V Q + R Sbjct: 61 IEVVVDDKVVEQAVDAIIKAARTGKIGDGKIFVQEVEQVIRIRT 104 >gi|300309430|ref|YP_003773522.1| nitrogen regulatory PII-like protein [Herbaspirillum seropedicae SmR1] Length = 112 Score = 144 bits (363), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 70/90 (77%), Positives = 80/90 (88%) Query: 1 MKQVTAIIKPFKLDEVRESLAEVGVTGLTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK 60 MK +TAIIKPFKLDEVRE+L+ +GV G+TVTEVKGFGRQKGHTELYRGAEYVVDFLPK K Sbjct: 1 MKLITAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKTK 60 Query: 61 IEVVVDDKVVEQAVDAIIKAARTGKIGDXR 90 IE VDD +VE+A++AI AARTGKIGD + Sbjct: 61 IEAAVDDAIVERALEAIETAARTGKIGDGK 90 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 222,807 Number of extensions: 4562 Number of successful extensions: 22 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 22 Number of HSP's successfully gapped: 2 Length of query: 113 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 40 Effective length of database: 1,274,215 Effective search space: 50968600 Effective search space used: 50968600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 124 (52.4 bits)