BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0114.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 766 0.0 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 238 1e-63 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 207 1e-54 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 204 1e-53 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 194 1e-50 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 766 bits (1977), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/405 (93%), Positives = 385/405 (95%), Gaps = 8/405 (1%) Query: 1 MNLADISKLGVRDPFKQRYDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVEL 60 MNLADISKLGVRDPFKQRYDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVEL Sbjct: 1 MNLADISKLGVRDPFKQRYDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVEL 60 Query: 61 ALDAAHAAKKSWGKTSPAERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPL 120 ALDAAHAAKKSWGKTSP ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPL Sbjct: 61 ALDAAHAAKKSWGKTSPTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPL 120 Query: 121 AIDHFRYFAAAVRTQEGSICTIDSDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALA 180 AIDHFRYFAAAVRTQEGSIC ID+DTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALA Sbjct: 121 AIDHFRYFAAAVRTQEGSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALA 180 Query: 181 AGNCVVLKPAEQTPASIMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTG 240 AGNCVVLKPAEQTPASIMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTG Sbjct: 181 AGNCVVLKPAEQTPASIMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTG 240 Query: 241 ETTTGRLIMQYASQNLIPVTLELGGKSPNIFFADVLDKDDEFFDKALEGFAMFALNQGEV 300 ETTTGRLIMQYASQNLIPVTLELGGKSPNIFFADVLDKDD+FFDKALEGFAMFALNQGEV Sbjct: 241 ETTTGRLIMQYASQNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEV 300 Query: 301 CTCPSRVLVQESIYDRFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGK 360 CTCPSRVLVQESIY+RFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGK Sbjct: 301 CTCPSRVLVQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGK 360 Query: 361 QEGAKVLAGGGREELGGTNLPSAWAARSRPAACGPTAITCIRRMR 405 QEGAKVLAGGGREELGG +L S + + PT +MR Sbjct: 361 QEGAKVLAGGGREELGG-DLASGYYVK-------PTVFQGNNKMR 397 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 238 bits (606), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 149/349 (42%), Positives = 203/349 (58%), Gaps = 21/349 (6%) Query: 19 YDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTSPA 78 + +FI G+F P K + ++I P G+ +++ SA DV+ A+ AA AA W Sbjct: 33 FGHFIAGRFTQP-KAD-LDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPGWQALGGH 90 Query: 79 ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGS 138 RA L +A ++ + LLA ETLDNGKP+RE+ D+PL HF ++A + QE Sbjct: 91 GRARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWAQLQESE 150 Query: 139 ICTIDSDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIM 198 + H P+GVVGQI+PWNFP+LM WK+APALA GN VVLKPAE T + + Sbjct: 151 ----------FPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTAL 200 Query: 199 VLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLI 257 + EL +P GV+NIV G G G + ++ I KIAFTG T GRLI + + + Sbjct: 201 LFAELAQQAGLPAGVLNIVTGDGA-TGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGK 259 Query: 258 PVTLELGGKSPNIFFADVLDKDDEFFDKALEGFA-MFALNQGEVCTCPSRVLVQESIYDR 316 +TLELGGKSP I F +D D A+EG NQG+VC SR+LVQE I+D Sbjct: 260 SLTLELGGKSPFIVF------EDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDL 313 Query: 317 FIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAK 365 FI R R+ +K G PLDK + +GA S QLE++ S ++ G +EGA+ Sbjct: 314 FIARLKTRMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREGAQ 362 Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 65/221 (29%), Positives = 94/221 (42%), Gaps = 6/221 (2%) Query: 22 FIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTSPAERA 81 +IGGK V P GE G +V + +D+ AA W SP RA Sbjct: 521 YIGGKQVRP-DGEVSMACHSTQGERLEDVGLGNRKDIR-NAVAAAVKAGGWTSASPHRRA 578 Query: 82 NMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSICT 141 L IA+ + A E A G E A ++ +I + A EG++ Sbjct: 579 QGLYYIAENLSARTEEFARRIASTTGVTADEARA-EVDASIKRLFTYGAWADKFEGAVHQ 637 Query: 142 IDSDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMVLI 201 A E +GVVG + P P+L + +AP +A GN VV+ P+EQ P L Sbjct: 638 PPMRGVALAMPEAIGVVGVVCPEEKPLLALISLVAPLIAMGNRVVVVPSEQAPLVATDLY 697 Query: 202 ELI--ADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTG 240 +++ +DL P GV+NIV G E LA + + + G Sbjct: 698 QVLDTSDL-PAGVINIVTGKSAELLPVLAEHDEVEALWVRG 737 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 207 bits (527), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 130/356 (36%), Positives = 189/356 (53%), Gaps = 13/356 (3%) Query: 18 RYDNF-IGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTS 76 R D+F I GK+ PP E ++P CE+A +A DV+ A+ AA A +W TS Sbjct: 14 RLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATS 73 Query: 77 PAERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQE 136 P ERA +L ++ + EL A A TL+ G PI A +PLA +H R R Sbjct: 74 PLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR----VARDNL 129 Query: 137 GSICTIDSDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPAS 196 I EP+GV I PWN+PI K+ PALAAG VVLKP+E +P S Sbjct: 130 ADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLS 189 Query: 197 IMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQN 255 ++ E++ + +P GV N+V G G E G L+++ ++ I+ TG T G L+ Q A+ Sbjct: 190 ALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVT 249 Query: 256 LIPVTLELGGKSPNIFFADVLDKDDEFFDKAL-EGFAMFALNQGEVCTCPSRVLVQESIY 314 + V ELGGKSPN+ D ++A+ G A N G+ C+ P+R++V + Sbjct: 250 VKRVAQELGGKSPNLVLPDA------DLERAIPPGVAAALRNMGQSCSAPTRLIVPRAAL 303 Query: 315 DRFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG 370 R E AL +A +K G+P D +T G A++ Q ++ I+ G Q+GA++LAGG Sbjct: 304 ARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGG 359 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 204 bits (518), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 136/358 (37%), Positives = 193/358 (53%), Gaps = 14/358 (3%) Query: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKK--SWGKTSPA 78 +FI G+ G+ ++P+ + A+ +A DV A+ AA A + +W K S Sbjct: 7 HFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSGL 66 Query: 79 ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEG- 137 +R +L K+A +E + +LLA + G+ E D+P AI H R A EG Sbjct: 67 QRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEGR 126 Query: 138 SICT---IDSDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTP 194 +I T + T +Y EP+GVVG I+PWN P+++ WK+A LAAG VV+KPAE+TP Sbjct: 127 TIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETP 186 Query: 195 ASIMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYAS 253 S + L L + P GV+N+V G+G G+ L + +AKI+FTG GR I + A Sbjct: 187 QSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAG 246 Query: 254 QNLIPVTLELGGKSPNIFFADVLDKDDEFFDKALEGFAM-FALNQGEVCTCPSRVLVQES 312 + VTLELGGKSP I F DD F+ AL G + +NQG+VC SR+LVQ S Sbjct: 247 EQFKRVTLELGGKSPQIVF------DDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS 300 Query: 313 IYDRFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG 370 + RF + + A I G+P +G A + Q E++ YI G EGA +LAGG Sbjct: 301 LAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG 358 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 194 bits (493), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 120/355 (33%), Positives = 192/355 (54%), Gaps = 15/355 (4%) Query: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAH-AAKKSWGKTSPAE 79 +FI ++V P GE + P G+ F +AR +A D++ A+ AA A + +WG+ S AE Sbjct: 4 HFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAAE 63 Query: 80 RANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSI 139 R+ +L+K+A +++ + E LA E D GKP+++ A D + +F ++A A G Sbjct: 64 RSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARA-DAAAIVRYFEFYAGAADKLHGDT 122 Query: 140 CTIDSDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMV 199 + EP GV G I+PWN+P+ + + ALAAGN V+KPAE SI+ Sbjct: 123 IPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILR 182 Query: 200 LIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIP 258 + EL A++ P G +NIV G+G EAG L + + I+FTG G+L+++ A+++ P Sbjct: 183 VAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTP 242 Query: 259 VTLELGGKSPNIFFADVLDKDDEFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYDRFI 318 VTLELGGKSP I F D D E + N G+ C+ SR+LVQ Y++ + Sbjct: 243 VTLELGGKSPQIVFEDA---DMEAMLPVV--VNAIVQNAGQTCSAGSRLLVQRGAYEQVL 297 Query: 319 ERALKRVAAIKQGNP---LDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG 370 ER + ++ G+ LD +I + Q E++ ++ +++G + A G Sbjct: 298 ERLSGLFSQLQVGSSQMDLDCGPLI----RKTQQERVAGFLSDAQRDGIALAAQG 348 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,205,225 Number of extensions: 49160 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 21 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)