BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0091.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311236|ref|YP_003775328.1| hypothetical protein Hsero_1911... 1029 0.0 gi|300313035|ref|YP_003777127.1| serine phosphatase, regulator o... 98 2e-21 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 81 2e-16 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 81 3e-16 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 80 3e-16 >gi|300311236|ref|YP_003775328.1| hypothetical protein Hsero_1911 [Herbaspirillum seropedicae SmR1] Length = 600 Score = 1029 bits (2660), Expect = 0.0, Method: Compositional matrix adjust. Identities = 491/522 (94%), Positives = 503/522 (96%) Query: 1 MTRFAQLGLPGNLFLNVSPGALMQPEAIQGETLRYLEKLGVNPQRIIIELTENEPTYDYA 60 +TRFAQLGLPGNLFLNVSPGALMQPEAIQGETLRYL+ LGVNPQRIIIELTENEPTYDYA Sbjct: 79 LTRFAQLGLPGNLFLNVSPGALMQPEAIQGETLRYLDTLGVNPQRIIIELTENEPTYDYA 138 Query: 61 LLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFVQGINHDPMKLQFVR 120 LRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFVQGINHDPMKLQFVR Sbjct: 139 QLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFVQGINHDPMKLQFVR 198 Query: 121 SIQEIAEKSGSIVIAEGIESQAELLTIRDLGIACGQGYHISRPLPEPPVSISDDVTKTLR 180 SIQEIAEKSGSIVIAEGIESQAELLTIRDLGIACGQGYHISRPLPEPPVSISDDVTKTLR Sbjct: 199 SIQEIAEKSGSIVIAEGIESQAELLTIRDLGIACGQGYHISRPLPEPPVSISDDVTKTLR 258 Query: 181 RDKVPLAANKYGPGKRSATVAKLLRETQYVSPEVTNDAAYDMFVNDPQLQALPVVSNGLP 240 RDKVPL A KYGPGK S TV KLLRET+YVSPEV+ND YDMFVNDPQLQALPVVSNGLP Sbjct: 259 RDKVPLDAKKYGPGKHSTTVFKLLRETRYVSPEVSNDTVYDMFVNDPQLQALPVVSNGLP 318 Query: 241 IGLINRYSMVERFARLYGRELYGKKSCAFFMDPNPILTEQDTSLQDLSNLLVHAESHHLS 300 IGLINRYSMVERFARLYGRELYGKKSCAFFMDPNPILTEQ TSLQDLSNLLVHAESHHLS Sbjct: 319 IGLINRYSMVERFARLYGRELYGKKSCAFFMDPNPILTEQSTSLQDLSNLLVHAESHHLS 378 Query: 301 NGFIITDRGQYVGLGTGHDLLREITQMQIDAARHANPLTQLPGNVPINDHIEYLLQSGVE 360 NGFIITDRGQY+GLGTGHDLLREITQMQIDAARHANPLTQLPGNVPINDHIEYLLQSGVE Sbjct: 379 NGFIITDRGQYMGLGTGHDLLREITQMQIDAARHANPLTQLPGNVPINDHIEYLLQSGVE 438 Query: 361 FCICYCDLDNFKPFNDVYGYRKGDDMIQTTGRVLQQACDPNLDFIGHIGGDDFLILFQSE 420 FCICYCDLD FKPFNDVYGYRKGDDMIQ TGRVLQQACDP LDFIGHIGGDDFLILFQSE Sbjct: 439 FCICYCDLDQFKPFNDVYGYRKGDDMIQATGRVLQQACDPELDFIGHIGGDDFLILFQSE 498 Query: 421 DWEKRCRTILDTFEAETRAFYYDEDVQRKGYYTEDRQGNRVFHPLVSLSLGSVKIEPSQY 480 DWEKRCR++LD F+AETR FYYDEDV+R GYYTEDRQGNRVFH LVS+SLG+VKIEPSQY Sbjct: 499 DWEKRCRSMLDNFDAETRGFYYDEDVERGGYYTEDRQGNRVFHKLVSVSLGAVKIEPSQY 558 Query: 481 SSPHQIASAATRAKKEAKKIQGNSLFIERRIPTTFSWALKDA 522 SSPHQIASAATRAKK+AKKI GNSLFIERRIP TFSWALKDA Sbjct: 559 SSPHQIASAATRAKKQAKKIPGNSLFIERRIPATFSWALKDA 600 >gi|300313035|ref|YP_003777127.1| serine phosphatase, regulator of sigma subunit [Herbaspirillum seropedicae SmR1] Length = 422 Score = 97.8 bits (242), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 49/136 (36%), Positives = 80/136 (58%) Query: 199 TVAKLLRETQYVSPEVTNDAAYDMFVNDPQLQALPVVSNGLPIGLINRYSMVERFARLYG 258 T L + V + N A ++F +L +LPVV PIGLI+R+ + + ++ Y Sbjct: 19 TAGALAVQVPVVQFDQNNAAVLEIFDQHKELVSLPVVEQQRPIGLISRHIFMSQMSKPYH 78 Query: 259 RELYGKKSCAFFMDPNPILTEQDTSLQDLSNLLVHAESHHLSNGFIITDRGQYVGLGTGH 318 RELY +KSC FMD +P++ + DT ++ L L V + L++GF+I ++GQ GLG+G Sbjct: 79 RELYERKSCIAFMDKDPLIVDADTPIETLGALAVASGDKTLADGFMIVEQGQLNGLGSGL 138 Query: 319 DLLREITQMQIDAARH 334 L+RE+ +Q++ R Sbjct: 139 ALMRELVDLQVEKNRQ 154 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 81.3 bits (199), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 46/127 (36%), Positives = 67/127 (52%) Query: 40 GVNPQRIIIELTENEPTYDYALLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYV 99 G P I IE+TE D +R+ + R G Q+A+DD G G+S++ + +Y+ Sbjct: 604 GCQPGLIAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSMDYL 663 Query: 100 KVDMHFVQGINHDPMKLQFVRSIQEIAEKSGSIVIAEGIESQAELLTIRDLGIACGQGYH 159 K+D FVQ + V +I +A + G VIAEG+E+ + +RD G QGY Sbjct: 664 KIDRSFVQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYL 723 Query: 160 ISRPLPE 166 SRPLPE Sbjct: 724 YSRPLPE 730 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 80.9 bits (198), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 43/130 (33%), Positives = 70/130 (53%) Query: 36 LEKLGVNPQRIIIELTENEPTYDYALLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQ 95 L++ + P + +E+TE+ D A +N +MGF ++IDD G G+SSL L Sbjct: 643 LQRHRLQPAALCLEITESAIMDDPARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLP 702 Query: 96 PEYVKVDMHFVQGINHDPMKLQFVRSIQEIAEKSGSIVIAEGIESQAELLTIRDLGIACG 155 + +K+D FV + D + VRS ++ G V+AEG+ES+ +R +G G Sbjct: 703 VDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQG 762 Query: 156 QGYHISRPLP 165 QGY + +P+P Sbjct: 763 QGYFMGKPMP 772 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 80.5 bits (197), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 48/153 (31%), Positives = 80/153 (52%), Gaps = 1/153 (0%) Query: 12 NLFLNVSPGALMQPEAIQGETLRYLEKLGVNPQRIIIELTENEPTYDYALLRNAAMHYRD 71 ++ +NVS L +P+ +Q L +E+ G NP+R+ +ELTE+ + + + Sbjct: 866 SIAVNVSAPQLREPDFVQ-TVLDTIERTGANPRRLKLELTESLLVDNVEDIIQKMFALKA 924 Query: 72 MGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFVQGINHDPMKLQFVRSIQEIAEKSGS 131 G + ++DD G G+SSL L +K+D FV+ + DP ++I +A+ G Sbjct: 925 YGVEFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQSLGL 984 Query: 132 IVIAEGIESQAELLTIRDLGIACGQGYHISRPL 164 VIAEG+E+ A+ + D G QGY RP+ Sbjct: 985 GVIAEGVETDAQRSFLADAGCHAYQGYFFCRPV 1017 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,674,126 Number of extensions: 71642 Number of successful extensions: 241 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 20 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)