BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0081.p (176 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311244|ref|YP_003775336.1| metal-binding, possibly nucleic... 305 2e-84 >gi|300311244|ref|YP_003775336.1| metal-binding, possibly nucleic acid-binding protein [Herbaspirillum seropedicae SmR1] Length = 166 Score = 305 bits (780), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 154/166 (92%), Positives = 157/166 (94%), Gaps = 1/166 (0%) Query: 12 MNAFVIDAFEFCRLKQQAQGEIPVAELGRLAQETVDRSGAIHWTLSGDIDKLGHAQLSLA 71 MNAFVIDAF+FCRLKQQAQGEIPVAELGRLAQETVDRSG I W LSGDIDKLGHAQLSLA Sbjct: 1 MNAFVIDAFDFCRLKQQAQGEIPVAELGRLAQETVDRSGTIRWALSGDIDKLGHAQLSLA 60 Query: 72 VSGAVNLICQRCLTALNFEIDSKSLLVLAKDEAQADTIEELLDDDEIDVIVGSAAFDVNY 131 VSGAVNLICQRCLTALNFEIDSKSLLVLAKDEAQAD IEELLDDDEIDVIVGSAAFDVNY Sbjct: 61 VSGAVNLICQRCLTALNFEIDSKSLLVLAKDEAQADAIEELLDDDEIDVIVGSAAFDVNY 120 Query: 132 LIEDDALLALPVAPKHDVCPD-GSVAAEAKGAERASPFEVLKKLKQ 176 LIED+ALLALPVAPKHD CP+ GS A EAK AERASPFEVLKKLKQ Sbjct: 121 LIEDEALLALPVAPKHDKCPEGGSAAVEAKSAERASPFEVLKKLKQ 166 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 473,888 Number of extensions: 16270 Number of successful extensions: 49 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 48 Number of HSP's successfully gapped: 1 Length of query: 176 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 97 Effective length of database: 1,245,805 Effective search space: 120843085 Effective search space used: 120843085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)