BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0076.p (251 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 484 e-138 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 188 4e-49 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 179 3e-46 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 158 5e-40 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 147 1e-36 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 484 bits (1246), Expect = e-138, Method: Compositional matrix adjust. Identities = 244/251 (97%), Positives = 250/251 (99%) Query: 1 MSALNLQGQVALVTGASRGIGRAIATELARQGAKVVGTATSESGAAAITEYLAALGPEAG 60 MSA NLQGQVALVTGASRGIGRAIATELARQGAKV+GTATSESGAAAITEYLAALGPEAG Sbjct: 1 MSAQNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAG 60 Query: 61 RGAVLNVNDAEASVALVEQVQKEFGALSILVNNAGITQDQLAMRMKDEEWDSVIATNLTA 120 RGAVLNVNDAEASVALVEQVQK+FG+LSILVNNAGITQDQLAMRMKDEEWDSVIATNLTA Sbjct: 61 RGAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTA 120 Query: 121 VGRLSRAVLRGMMKARSGRIISITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRN 180 VGRLSRAVLRGMMKAR+GRII+ITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRN Sbjct: 121 VGRLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRN 180 Query: 181 ITVNCVAPGFIDTDMTKALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGT 240 ITVNCVAPGFIDTDMT+ALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGT Sbjct: 181 ITVNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGT 240 Query: 241 TLNVNGGMYMG 251 TLNVNGGMYMG Sbjct: 241 TLNVNGGMYMG 251 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 188 bits (477), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 102/246 (41%), Positives = 152/246 (61%), Gaps = 6/246 (2%) Query: 9 QVALVTGASRGIGRAIATELARQGAKVV---GTATSESGAAAITEYLAALGPEAGRGAVL 65 ++A VTG GIG AI T L + G VV G ++ T A G Sbjct: 4 RIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG--- 60 Query: 66 NVNDAEASVALVEQVQKEFGALSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLS 125 NV++ E++ A E+V+ E G + +LVNNAGIT+D +M ++WD+VI TNL ++ ++ Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 126 RAVLRGMMKARSGRIISITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNC 185 + V+ GM + GRII+I+SV G+ G GQ NY+ AKAG+ G + ALA+E+ ++ +TVN Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 Query: 186 VAPGFIDTDMTKALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVN 245 V+PG+I TDM +A+ + I+ IP+ RLG EEIA VA++AS + Y TG+ ++N Sbjct: 181 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLN 240 Query: 246 GGMYMG 251 GG+YMG Sbjct: 241 GGLYMG 246 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 179 bits (453), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 112/240 (46%), Positives = 151/240 (62%), Gaps = 8/240 (3%) Query: 12 LVTGASRGIGRAIATELARQGAKVVGTATSESGAA-AITEYLAALGPEAGRGAVLNV-ND 69 VTG+SRGIGRAIA LAR+G +V + AA + +AALG + R +V N Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQV-RVLQFDVANR 68 Query: 70 AEASVALVEQVQKEFGALSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAVL 129 +A+ L+ V++ G +V NAGIT+D M E+WDSV+ TNL + ++ Sbjct: 69 EQAAQVLLADVEQH-GCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLV 127 Query: 130 RGMMKARS-GRIISITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAP 188 M++ R+ GRI+++ SV G GN GQ+NY+AAKAGV G ++ALA E+ R+ITVNCVAP Sbjct: 128 MPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAP 187 Query: 189 GFIDTDMTKALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGGM 248 G IDTDMT+ + IL+ IP R G EE+A AVAFL SP AAYIT ++VNGG+ Sbjct: 188 GLIDTDMTE---DAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 158 bits (399), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 102/252 (40%), Positives = 149/252 (59%), Gaps = 13/252 (5%) Query: 6 LQGQVALVTGAS--RGIGRAIATELARQGAKVV--GTATSESGAAAITEYLAALGPEAGR 61 L+ +VA++TG + G+G + A +A QGA++V +E AA A LG + Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAA-----ARLGAQH-L 56 Query: 62 GAVLNVNDAEASVALVEQVQKEFGALSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAV 121 G V +V D A A V K+FG + ILVNNAG+TQ + + +++D ++ +L Sbjct: 57 GLVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGT 116 Query: 122 GRLSRAVLRGMMKARSGRIISITSVVGEAGNP--GQMNYAAAKAGVAGMSRALAREIGSR 179 +S+AVL M +SG I+ I+SV + G G +Y+AAKAGV G++RA+ARE G + Sbjct: 117 LYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQ 176 Query: 180 NITVNCVAPGFIDTDMTK-ALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYIT 238 I VNC+ PG I TD+ K L+ ++ A I Q IPLGRLG +++ FLAS + Y T Sbjct: 177 GIRVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCT 236 Query: 239 GTTLNVNGGMYM 250 G TL+VNGGM + Sbjct: 237 GVTLDVNGGMLI 248 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 147 bits (370), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 98/250 (39%), Positives = 143/250 (57%), Gaps = 9/250 (3%) Query: 6 LQGQVALVTGAS--RGIGRAIATELARQGAKVVGTATSESGAAAITEYLAALGPEAGRGA 63 L+ +VA++TG + G+G A A +A QGA+VV + A A +G + G Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVV---VLDLAQADPAAAAARIGSQH-LGL 58 Query: 64 VLNVNDAEASVALVEQVQKEFGALSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGR 123 V +V D + + ++FG + ILVNNAGITQ + + ++D ++ +L Sbjct: 59 VADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLY 118 Query: 124 LSRAVLRGMMKARSGRIISITSVVGEAGNP--GQMNYAAAKAGVAGMSRALAREIGSRNI 181 +S+AVL M ++G II I+SV + G G +Y+AAKAGV G++RA+ARE G I Sbjct: 119 MSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGI 178 Query: 182 TVNCVAPGFIDTDMTKA-LNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGT 240 VN + PG I TD+ K L E++ A I + IPL RLG A++I A FL S + Y TG Sbjct: 179 RVNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGI 238 Query: 241 TLNVNGGMYM 250 TL+VNGGM + Sbjct: 239 TLDVNGGMLI 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.343 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 719,676 Number of extensions: 28746 Number of successful extensions: 324 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 49 Length of query: 251 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 168 Effective length of database: 1,226,865 Effective search space: 206113320 Effective search space used: 206113320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)