BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0068.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 872 0.0 gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 415 e-117 gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 233 3e-62 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 90 5e-19 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 872 bits (2254), Expect = 0.0, Method: Compositional matrix adjust. Identities = 471/493 (95%), Positives = 481/493 (97%), Gaps = 1/493 (0%) Query: 1 MTPMKNTLAGALLAASVFAAAPAVQAAPPVASPAVAALPDFADIVERTGPAVVNIRTTER 60 MTP+K TLA ALLAASVF AAP AAPPVASPAVAALPDFADIVERTGP VVNIRTTER Sbjct: 1 MTPLKTTLASALLAASVFVAAPVALAAPPVASPAVAALPDFADIVERTGPGVVNIRTTER 60 Query: 61 VRQNAQGQGGGMDDPEMQEFFRRFFGIPIPRQQQPGTPRGNGKQGGQGQQEEVPRGVGSG 120 VRQNAQGQGG +DDPEMQEFFRRFFGIPIPRQQQPGTPRGNGKQGGQGQQEEVPRGVGSG Sbjct: 61 VRQNAQGQGG-VDDPEMQEFFRRFFGIPIPRQQQPGTPRGNGKQGGQGQQEEVPRGVGSG 119 Query: 121 FIISADGFIMTNAHVVDGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPRLNM 180 FIISADGFIMTNAHVV+GASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPRLNM Sbjct: 120 FIISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPRLNM 179 Query: 181 GDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNSGGPLI 240 GDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNSGGPLI Sbjct: 180 GDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNSGGPLI 239 Query: 241 NLKGEVVGINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGEVTKD 300 NLKGEV+GINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGEVTKD Sbjct: 240 NLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGEVTKD 299 Query: 301 VAESLGLARAQGALVQRVEAGGPADKAGLEAGDIILKYNGAAIERPSDLPRMVGATKPGA 360 VAESLGLARAQGALVQRVEAGGPA+KAGLEAGDIILKYNGAAIERPSDLPRMVG+TKPGA Sbjct: 300 VAESLGLARAQGALVQRVEAGGPAEKAGLEAGDIILKYNGAAIERPSDLPRMVGSTKPGA 359 Query: 361 KATVNIWRKGSARDVSVTVVELEADKPKQAEEKKAKPDNTPNSLGLVVSDLSDAQKKELK 420 KATV+IWRKGSARDVSVTVVELEADKPKQAEEKKAKPDN PNSLGLVVSDLSDAQ+KELK Sbjct: 360 KATVSIWRKGSARDVSVTVVELEADKPKQAEEKKAKPDNAPNSLGLVVSDLSDAQRKELK 419 Query: 421 VDNGVLVENVSGAAAAAGIRPGDVIQRLNEVDVNDSKQFGQLVAKLDAKKRAAVLVRRGD 480 VDNGVLVE VSGAAAAAGIRPGDVIQRLNEVDV D+KQF QLV+KLDAKKRAAVLVRRGD Sbjct: 420 VDNGVLVEAVSGAAAAAGIRPGDVIQRLNEVDVKDAKQFAQLVSKLDAKKRAAVLVRRGD 479 Query: 481 SSQFVPLRPNGGN 493 SSQFVPLRPNGGN Sbjct: 480 SSQFVPLRPNGGN 492 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 415 bits (1067), Expect = e-117, Method: Compositional matrix adjust. Identities = 227/455 (49%), Positives = 302/455 (66%), Gaps = 28/455 (6%) Query: 40 DFADIVERTGPAVVNIRTTERVRQNAQGQGGGMD--DPEMQEFFRRFFGIPIPRQQQPGT 97 DF+ IV+R GPAVVNI T + R+ A G+D DP EFF+RF P+ Q P Sbjct: 64 DFSGIVQRYGPAVVNISVTGKARKVADDDDNGLDPNDP-FSEFFKRFG----PQLQMPQQ 118 Query: 98 PRGNGKQGGQGQQEEVPRGVGSGFIISADGFIMTNAHVVDGASEVYVTLTDKREFKAKIV 157 PR + G+GSGFIIS DG I+TNAHVVDGA EV V LTD+REFKAK++ Sbjct: 119 PR-------------IMHGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFKAKVL 165 Query: 158 GSDTRTDVAVLKIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDT 217 G D ++D+AV++ID NLP + +GD +++VG+ VLAIGSP+G +NT TAGI+SAK+R Sbjct: 166 GIDKQSDIAVIRIDAKNLPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGIISAKSRSL 225 Query: 218 GD--YLPLIQTDVAVNPGNSGGPLINLKGEVVGINSQIYSRSGGFMGISFAVPIDEALRV 275 D Y+P IQTDVAVNPGNSGGPL +L G+V+GINSQIYS++GGF G+SF++PID A++V Sbjct: 226 PDDNYVPFIQTDVAVNPGNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKV 285 Query: 276 ADQLKASGRVTRGRIGVQIGEVTKDVAESLGLARAQGALVQRVEAGGPADKAGLEAGDII 335 QL G+VTRGR+GV + ++ + +++S GL +A+GALV VE G PADKAGL+AGD+I Sbjct: 286 EQQLVTHGKVTRGRLGVSVQDLNQALSDSFGLKKAEGALVSSVEKGSPADKAGLQAGDVI 345 Query: 336 LKYNGAAIERPSDLPRMVGATKPGAKATVNIWRKGSARDVSVTVVELEADKPKQAEEKKA 395 + +NG AI+ DLP +V T PG+ + + R G R ++VTV E++ K + KA Sbjct: 346 ISFNGHAIDHSVDLPTLVADTPPGSTKPMEVMRAGKVRTINVTVGEMKQAK----NDAKA 401 Query: 396 KPDNTPNSLGLVVSDLSDAQKKELKVDNGVLVENVSGAAAAAGIRPGDVIQRLNEVDVND 455 + LGL V L ++ +L G+LVE+VSG AA AGI+ GDVI +N V+ Sbjct: 402 SKSDDQGRLGLAVRPLDKDEQAQLGGQKGLLVEDVSGPAAVAGIQSGDVILAVNGTPVSS 461 Query: 456 SKQFGQLVAKLDAKKRAAVLVRRGDSSQFVPLRPN 490 Q +L DA K A+LV RGD FVPL N Sbjct: 462 VAQLRKLAT--DAGKNVALLVERGDEKIFVPLALN 494 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 233 bits (594), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 139/353 (39%), Positives = 207/353 (58%), Gaps = 28/353 (7%) Query: 31 ASPAVAALPDFADIVERTGPAVVNIRTTERVR-QNAQGQGGGMDDPEMQEFFRRFFGIPI 89 A+P AL + + P+VVNI TT R Q + Q +DP FFR+FFG Sbjct: 50 ATPNAPALDSYRHAARQAMPSVVNIFTTTEARPQKSPFQ----NDP----FFRKFFG--- 98 Query: 90 PRQQQPGTPRGNGKQGGQGQQEEVPRGVGSGFIISADGFIMTNAHVVDGASEVYVTLTDK 149 Q + QQ++ +GSG I+S G+I+TN HVV+ A ++ V L D Sbjct: 99 -------------DQFDEQQQDDKQSSLGSGVIVSPQGYILTNNHVVEAADKIEVALADG 145 Query: 150 REFKAKIVGSDTRTDVAVLKIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGI 209 R+ AK+VG D TD+AV+KID NLP + +G + VG+ VLAIG+PFG+ TVT GI Sbjct: 146 RKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPENSSVGDVVLAIGNPFGVGQTVTMGI 205 Query: 210 VSAKARD---TGDYLPLIQTDVAVNPGNSGGPLINLKGEVVGINSQIYSRSGGFMGISFA 266 VSA R+ + IQTD A+NPGNSGG L++ G ++GIN+ IYSR+GG +GI FA Sbjct: 206 VSALGRNHLGINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFA 265 Query: 267 VPIDEALRVADQLKASGRVTRGRIGVQIGEVTKDVAESLGLARAQGALVQRVEAGGPADK 326 +P+ A V + + + G+V RG IGV+ ++T ++AES GL + GA++ V GGPAD+ Sbjct: 266 IPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPELAESFGLGKKTGAIIAGVLKGGPADR 325 Query: 327 AGLEAGDIILKYNGAAIERPSDLPRMVGATKPGAKATVNIWRKGSARDVSVTV 379 AG+ GDI++ + + +++ ++ PG + + + RK +++TV Sbjct: 326 AGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQIAMTVLRKSQETKLNITV 378 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 89.7 bits (221), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 63/167 (37%), Positives = 97/167 (58%), Gaps = 9/167 (5%) Query: 118 GSGFIISADGFIMTNAHVVDGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPR 177 G+GF I G++++N HVV A V V L RE A+++ D DVA+LK + + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 178 LNMGDSDKIRVGEWVLAIGSPFG--LENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNS 235 L + D+ +GE V A+GSP G T+T GI+S G +Q+DVA+ PG+S Sbjct: 331 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILSGVRLLNGQE--FLQSDVAILPGSS 387 Query: 236 GGPLINLKGEVVGIN-SQIYSRSGGFMGISFAVPIDEAL-RVADQLK 280 GGPL++ G+VVGI + + +R G G++F +P+ A+ R+ QL+ Sbjct: 388 GGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASAVERLNLQLQ 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,349,233 Number of extensions: 55484 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 4 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)