BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0041.p (552 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 869 0.0 gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 225 6e-60 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 206 5e-54 gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 171 1e-43 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 869 bits (2246), Expect = 0.0, Method: Compositional matrix adjust. Identities = 468/504 (92%), Positives = 481/504 (95%) Query: 1 MPHHTPLIATIVGGLVLAFIFGTLAHRLKMPPLIGYLVAGIAIGPFTPGFVGDADLAQEL 60 MPHHTPLIATIV GLVLAFIFGTLAHRLKMPPL+GYLVAGIAIGPFTPGFVGDADLAQEL Sbjct: 1 MPHHTPLIATIVAGLVLAFIFGTLAHRLKMPPLVGYLVAGIAIGPFTPGFVGDADLAQEL 60 Query: 61 AEIGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGWLLGWPFGEGFLF 120 AEIGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQI IATLLGM LGWLLGWP GEGFLF Sbjct: 61 AEIGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQITIATLLGMALGWLLGWPVGEGFLF 120 Query: 121 GLALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGE 180 GLALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGE Sbjct: 121 GLALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGE 180 Query: 181 ALSGNAVLLTLGLTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGF 240 ALSGNAVLLTLG+TLGKVVAF AVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGF Sbjct: 181 ALSGNAVLLTLGITLGKVVAFAAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGF 240 Query: 241 ALGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIL 300 ALGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSI+ Sbjct: 241 ALGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIV 300 Query: 301 VREPLLVLATVLIIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSL 360 REPLLVLATVLIIVVGKSL A LIVRAFG+PN TALTISASLAQIGEFSFILATLGLSL Sbjct: 301 AREPLLVLATVLIIVVGKSLGALLIVRAFGYPNGTALTISASLAQIGEFSFILATLGLSL 360 Query: 361 DLLSPMARDLILGGAILSILLNPLLFSLLDRYEARHAEPADVQDVPPTVDLQDHVVLVGY 420 DLLSPMARDLILGGAILSILLNPLLFSLLDRYEAR + D P V+LQDHVVLVG+ Sbjct: 361 DLLSPMARDLILGGAILSILLNPLLFSLLDRYEARQPKAPQGGDEPQKVELQDHVVLVGF 420 Query: 421 GRVGRGIGEKLRAQGKPFVVIEAQREHLDALRQDGVPALYGNAAQSELLKAAAVERARWL 480 GRVGRGIGE+LRAQGKPFVVIEAQ E+L+ALR GVPALYGNAAQSELL+AAA++RARWL Sbjct: 421 GRVGRGIGEQLRAQGKPFVVIEAQLENLEALRAAGVPALYGNAAQSELLQAAAIDRARWL 480 Query: 481 LVAIPEVFEAGQVIENALELNAGL 504 LVAIPEVFEAGQVIENALELN GL Sbjct: 481 LVAIPEVFEAGQVIENALELNPGL 504 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 225 bits (574), Expect = 6e-60, Method: Compositional matrix adjust. Identities = 171/503 (33%), Positives = 262/503 (52%), Gaps = 26/503 (5%) Query: 15 LVLAFIFGTLAHRL-KMPPLIGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVG 73 L+ A + G +A R+ ++PP++GYLV GI IGP GF D + L E GV+ LMF +G Sbjct: 11 LLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLGFAEDNETTHALGEFGVVFLMFSIG 70 Query: 74 LHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGW--------LLGWPFGEGFLFGLALS 125 L FSL L ++++I VAQ+A M LGW + F G AL+ Sbjct: 71 LEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQAAFALGGALT 130 Query: 126 VASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGN 185 ++ST ++ K L ER +ET+ GR +G L+ +D+A+V L+++PAL+ SGN Sbjct: 131 MSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALAN--------ESGN 182 Query: 186 AVLLTLGLTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSA 245 +++TLG GK + +A++L G +++ + S+ELF L +L I LG A + Sbjct: 183 -IMVTLGWAAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWITE 241 Query: 246 YVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSILVREPL 305 G+S ALGAF AGM++SE+E H+ E+ RD LFF+++GML + ++ Sbjct: 242 KA-GLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHWF 300 Query: 306 LVLATVLIIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLDLLSP 365 LVL + V+ K + R FG AL + LAQ GEF F+L + L L+ P Sbjct: 301 LVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDP 360 Query: 366 MARDLILGGAILSILLNPLLFSLLD----RYEARH---AEPADVQDVPPTVDLQDHVVLV 418 M +IL +LS+L P + + D ++ A A Q T+ +Q HV++ Sbjct: 361 MLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVIIA 420 Query: 419 GYGRVGRGIGEKLRAQGKPFVVIEAQREHLDALRQDGVPALYGNAAQSELLKAAAVERAR 478 G+GR G+ + L +G + +E E + R G YG+AA+ E L AA + RA Sbjct: 421 GFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAA 480 Query: 479 WLLVAIPEVFEAGQVIENALELN 501 L+V A +V+ +A EL Sbjct: 481 ALVVTYASTPSALKVLHHAAELE 503 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 206 bits (523), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 141/383 (36%), Positives = 212/383 (55%), Gaps = 12/383 (3%) Query: 15 LVLAFIFGTLAHRLKMPPLIGYLVAGIAIGPFTPGF--VGDADLAQELAEIGVILLMFGV 72 +++A I + ++LK P ++GY+VAG+ IGP TP + + D L+E+GVI L+F + Sbjct: 15 MLIAGIVTVVFNKLKQPVVLGYIVAGVIIGPHTPPYDLIQDEKTVHILSELGVIFLLFSL 74 Query: 73 GLHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGWLLGWPFGEGFLFGLALSVASTVVL 132 GL FSLK L V A GA A+I + +G +G GW + G L+V+ST ++ Sbjct: 75 GLEFSLKKLAKVGATAFIGAAAEILLMIWIGYEIGLYFGWKRMDAIFLGAMLAVSSTTII 134 Query: 133 LKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGNAVLLTLG 192 +KAL E + + +I G LIVEDI LA+ +I LSG+ A SG+ + Sbjct: 135 VKALNELGMKNEKFAQIIFGILIVEDI---LAIGMIALLSGV------ATSGSVDSTEVF 185 Query: 193 LTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSF 252 T+GK+V F+ V L+VG +P +L I+ S E+ +AVL + GF L S Sbjct: 186 TTVGKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCL-LVMKLQYSV 244 Query: 253 ALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSILVREPLLVLATVL 312 ALGAF G +++ES HR P+RD FS +FFV++G+LFDP++LV+ + + L Sbjct: 245 ALGAFLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNVLVKYWMPITVITL 304 Query: 313 IIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLIL 372 +V GK ++ L G T + + LAQIGEFSFI+A LG SL + S +++ Sbjct: 305 AVVFGKLISCGLGTFIAGQGGRTPMRVGMGLAQIGEFSFIIAALGQSLKVTSDFLYPIVV 364 Query: 373 GGAILSILLNPLLFSLLDRYEAR 395 + ++ LL P L D A+ Sbjct: 365 AVSAVTALLTPYLIKAADPLSAK 387 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 171 bits (434), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 151/477 (31%), Positives = 245/477 (51%), Gaps = 12/477 (2%) Query: 24 LAHRLKMPPLIGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLS 83 +A RL + ++GYL+AGI IGP+ G + + E +E GV+LL+F +GL K L + Sbjct: 21 IAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVLLLFLIGLELDPKRLWA 80 Query: 84 VKNIAIPGAVAQIAIATLLGMGLGWLLGWPFGEGFLFGLALSVASTVVLLKALQERRLVE 143 ++ Q+A +L + P+ G + L +S++ST + L L ER+L Sbjct: 81 LRRSIFGWGSIQVAAVSLALLAAALAASVPWRIGLIGALGMSLSSTAIALATLGERKLTA 140 Query: 144 TQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGNAVLLTLGLTLGKVVAFVA 203 T G A L+ +DIA + + +P L G+ +G SG L G+V +A Sbjct: 141 TPAGSAAFSILLFQDIAAIPMMAAVPLL-GVAASQG---SGQGWLHA-----GQVALVIA 191 Query: 204 VMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSFALGAFFAGMIL 263 +++ GR ++ IL IA T RE+F L + + L V G+S ALG F AG++L Sbjct: 192 LLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSV-GMSMALGTFLAGVLL 250 Query: 264 SESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSILVREPLLVLATVLIIVVGKSLAAW 323 ++SE H + P + LFF++VGM D + +P +L VL + K+ W Sbjct: 251 ADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLGFLAIKTCVMW 310 Query: 324 LIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILGGAILSILLNP 383 + R FG P L + L+Q GEF+FI+ ++ +L+P L++ LS+L+ P Sbjct: 311 ALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVVVVALSMLVTP 370 Query: 384 LLFSLLDRYEARHAEPADVQDVPPTVDLQDH-VVLVGYGRVGRGIGEKLRAQGKPFVVIE 442 LL + DR R E + +D QDH V++ G+GR G+ IG L A G V++ Sbjct: 371 LLLLVHDRLLVRWMEGGK-RRPEDKIDPQDHAVIIAGFGRFGQIIGRLLAANGIGATVLD 429 Query: 443 AQREHLDALRQDGVPALYGNAAQSELLKAAAVERARWLLVAIPEVFEAGQVIENALE 499 + +D LR+ G YG+A + +LL+ A ++AR L++AI + A ++++ E Sbjct: 430 HDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEMVDKVRE 486 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,363,863 Number of extensions: 48518 Number of successful extensions: 335 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 326 Number of HSP's successfully gapped: 4 Length of query: 552 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 462 Effective length of database: 1,193,720 Effective search space: 551498640 Effective search space used: 551498640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)