BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0040.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311287|ref|YP_003775379.1| 6,7-dimethyl-8-ribityllumazine ... 292 1e-80 gi|300312880|ref|YP_003776972.1| 6,7-dimethyl-8-ribityllumazine ... 61 6e-11 >gi|300311287|ref|YP_003775379.1| 6,7-dimethyl-8-ribityllumazine synthase [Herbaspirillum seropedicae SmR1] Length = 169 Score = 292 bits (747), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 155/169 (91%), Positives = 161/169 (95%), Gaps = 2/169 (1%) Query: 6 QSHLSAVPSHHGQRIAFVQASWHKDIVDQCRIAFTEEMAKVGWPTSSIDFFELPGAFEIP 65 QSHLSAV SHHGQRIAF+QA WHKDIVDQCRIAFTEEMAK+GWP +SIDFFELPGAFEIP Sbjct: 3 QSHLSAVSSHHGQRIAFIQACWHKDIVDQCRIAFTEEMAKLGWPAASIDFFELPGAFEIP 62 Query: 66 LHAKVLAKTGKYAGIVASGLVVDGGIYRHDFVAQSVISALMQVQLETEVPVFSAVLTPHH 125 LHAKVLAKTG+YAGIVASGLVVDGGIYRHDFVAQSVISALMQVQL+TEVPVFSAVLTPHH Sbjct: 63 LHAKVLAKTGRYAGIVASGLVVDGGIYRHDFVAQSVISALMQVQLDTEVPVFSAVLTPHH 122 Query: 126 FHSHSEHHDYFHQHFLVKGREAAHACATTVAKLAEVRAADAAQRSLNAA 174 FHSHSEHHDYFHQHFLVKGREAA ACATTVAKLA+VRA AQ SLNAA Sbjct: 123 FHSHSEHHDYFHQHFLVKGREAAQACATTVAKLAQVRA--GAQASLNAA 169 >gi|300312880|ref|YP_003776972.1| 6,7-dimethyl-8-ribityllumazine synthase [Herbaspirillum seropedicae SmR1] Length = 161 Score = 60.8 bits (146), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 43/149 (28%), Positives = 74/149 (49%), Gaps = 10/149 (6%) Query: 13 PSHHGQ--RIAFVQASWHKDIVDQCRIAFTEEMAKVGWPTSSIDFFELPGAFEIPLHAKV 70 P +G+ RI VQA +++D+ A E+ +G I +PGA EIPL + Sbjct: 8 PDFNGESLRIGIVQARFNEDVCHGLLAACLAELKHLGVADEDILHVTVPGALEIPLALQK 67 Query: 71 LAKTGKYAGIVASGLVVDGGIYRHDFVAQSVISALMQVQLETEVPVFSAVLTPHHFHSHS 130 +A+T ++ ++A G V+ G Y + V+ + + ++ L+ VP+ +AVLT + Sbjct: 68 MAETEQFDALIALGAVIRGETYHFELVSNESGAGITRIGLDFGVPIANAVLT-------T 120 Query: 131 EHHDYFHQHFLVKGREAAHACATTVAKLA 159 E+ + KG +AA A +A LA Sbjct: 121 ENDEQAEVRMAEKGADAAR-VAVEMANLA 148 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.131 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 444,216 Number of extensions: 15170 Number of successful extensions: 41 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 41 Number of HSP's successfully gapped: 2 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 127 (53.5 bits)