BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0039.p (186 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311289|ref|YP_003775381.1| lipoprotein protein [Herbaspiri... 323 5e-90 >gi|300311289|ref|YP_003775381.1| lipoprotein protein [Herbaspirillum seropedicae SmR1] Length = 185 Score = 323 bits (829), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 160/186 (86%), Positives = 166/186 (89%), Gaps = 1/186 (0%) Query: 1 MPRPRFSVPFFVITAATLGLTACTTGKTAFYHQEEFNDTSAFSRSFPGSEKAVCEAARRA 60 M PR V +L L AC T K AFYHQEEF+DTSAFSRSFPGSEKAVCEAARRA Sbjct: 1 MLHPRLPVSLCFAATLSLTLAACGT-KAAFYHQEEFSDTSAFSRSFPGSEKAVCEAARRA 59 Query: 61 LLGQGYVISKSTDNTLDGNKSFQPASDKHMEIAFHVVCASNGNGKSSTMFASATQDGYGI 120 LLGQGY+I+K+TDNT+DGNKSFQPASDKHMEIAFHVVCASNGNGKSSTMF SATQDGYGI Sbjct: 60 LLGQGYIITKATDNTMDGNKSFQPASDKHMEIAFHVVCASNGNGKSSTMFVSATQDGYGI 119 Query: 121 KKSNSSASLGVSVLGSVSMPFAASDDSMVRVSSETIRSPDFYDRFFGAAERYLLVDVDDD 180 KKSNSSASLGVSVLGSVSMPFAASDDSMVRVSSETIRS DFYDRFFGAAERYLLVDVDDD Sbjct: 120 KKSNSSASLGVSVLGSVSMPFAASDDSMVRVSSETIRSSDFYDRFFGAAERYLLVDVDDD 179 Query: 181 TAPAKK 186 TAP KK Sbjct: 180 TAPGKK 185 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 464,429 Number of extensions: 13972 Number of successful extensions: 31 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 31 Number of HSP's successfully gapped: 1 Length of query: 186 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 106 Effective length of database: 1,241,070 Effective search space: 131553420 Effective search space used: 131553420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)