BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0037.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [H... 578 e-166 gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 102 4e-23 gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop... 56 3e-09 >gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 578 bits (1491), Expect = e-166, Method: Compositional matrix adjust. Identities = 276/288 (95%), Positives = 283/288 (98%) Query: 12 MTEAVVKTVQCISPAGLHTMAYKEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRV 71 MTEAVVKTVQCISPAGLHTMAYKEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRV Sbjct: 1 MTEAVVKTVQCISPAGLHTMAYKEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRV 60 Query: 72 ICPDVVGRGRSGRLANPQFYTVPQYVSDMVTLLARADAEIVDWFGTSMGGLIGMGLASLP 131 ICPDVVGRGRSGRLANPQFYTVPQYVSDMVTLLAR DA+IVDWFGTSMGGLIGM LASLP Sbjct: 61 ICPDVVGRGRSGRLANPQFYTVPQYVSDMVTLLARLDAQIVDWFGTSMGGLIGMALASLP 120 Query: 132 GNPIRRLVLNDIGPSINGAALARIGDYIGQDVRFDTFEEAAQFIRTISFSFGQHSDEEWH 191 GNPIRRLVLNDIGPSINGAALARIGDYIGQDVRFD+FEEAAQ+IRTIS SFGQHSDEEWH Sbjct: 121 GNPIRRLVLNDIGPSINGAALARIGDYIGQDVRFDSFEEAAQYIRTISASFGQHSDEEWH 180 Query: 192 KLAKDVLRQNEEGKWVRHYDLGLAVPFKLTTPEAASAAEQMLWAAYDAIRCPTLVVRGAQ 251 KLAKDVLRQNE+G+WVRHYDL LA+PFK TTPEAASAAEQMLWAAYDAIRCPTL+VRGAQ Sbjct: 181 KLAKDVLRQNEQGQWVRHYDLALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQ 240 Query: 252 SDLLLPEVAQEMTRRGPKAKLVELPDVGHAPTFMHAPQIEVARQFLLG 299 SDLLLPEVAQEMTRRGPKAKLVELPDVGHAPTFMHAPQIEVARQFLLG Sbjct: 241 SDLLLPEVAQEMTRRGPKAKLVELPDVGHAPTFMHAPQIEVARQFLLG 288 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 102 bits (254), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 83/270 (30%), Positives = 132/270 (48%), Gaps = 18/270 (6%) Query: 35 EWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRVICPDVVGRGRSGRLANPQFYTVP 94 EWGD + +L+ +HG+ + F +A+ L YR I D GRG+S A +YT Sbjct: 19 EWGDPQGFPLLL-LHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWDAGANYYT-D 76 Query: 95 QYVSDMVTLLARADAEIVDWFGTSMGGLIGMGLASLPGNPIRRLVLNDIGPSI--NGAAL 152 YV+D+ L+AR + D G SMGG+ + A+ +RRL++ D GP N Sbjct: 77 AYVADVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAGPGAFENSPGA 136 Query: 153 ARIGDYIGQD-VRFDTFEEAAQFIRTISFSFGQHSDEEWHKLAKDVLRQNEEGKWVRHYD 211 RI + F +++EA+ F+R + S ++E + ++L+ +G + YD Sbjct: 137 TRIRRELATTPSHFASWDEASTFMRALRPSV---TEEARQQRLHNMLKPAAQGGYTWRYD 193 Query: 212 LGLAVPFKLTTPEAASAAEQMLWAAYDAIRCPTLVVRGAQSDLLLPEVAQEMTRRGPKAK 271 + + +P+ + + L A+ CPTLVVRG +SD L E+A EM + + Sbjct: 194 -HVGISSTRLSPDLSRVVD--LRPHIMALMCPTLVVRGERSDYLSAEMAAEMRQLNARIL 250 Query: 272 LVELPDVGHAPTFMHAPQ----IEVARQFL 297 + D GH ++H Q EV RQFL Sbjct: 251 TSTIADAGH---YIHDDQPEAFAEVVRQFL 277 >gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum seropedicae SmR1] Length = 300 Score = 56.2 bits (134), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 75/283 (26%), Positives = 120/283 (42%), Gaps = 34/283 (12%) Query: 34 KEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRVICPDVVGRGRSGRLANPQFYTV 93 + WGD P + + +HG +S F M L + VI PD G G + R + Y Sbjct: 25 RHWGDPEAPKIFM-LHGWMDMSASFQFMVDALQGQWHVIAPDWRGFGLTER-SQADSYWF 82 Query: 94 PQYVSDMVTLLAR-ADAEIVDWFGTSMGGLIGMGLASLPGNPIRRLV-LNDIG-PSIN-G 149 P Y++D+ ++ A +E ++ G SMGG + A + I+RL+ L +G P+ Sbjct: 83 PDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGVRPQRIKRLINLEGLGLPATQPE 142 Query: 150 AALARIGDYIGQDVR-------FDTFEEAAQFIRTISFSFGQHSDEEWHKLAKDVLRQNE 202 A R ++ +VR + T EE AQ ++ + SDE LA+ +Q++ Sbjct: 143 QAPGRFAQWM-DEVRNRPVLRGYATLEEVAQRLQRNNARL---SDERAAFLAQHWAQQDD 198 Query: 203 EGKWVRHYDLGLAVPFKLTTPEAASAAEQMLWAAYDAIRCPTLVVRGAQSDLL-----LP 257 G W D K +P A E M A + I P L + SD+ Sbjct: 199 SGLWQILGDPA----HKQVSPILYRADEMM--ACWRRITAPVLWIEAIDSDIWRFFGPAS 252 Query: 258 EVAQEMTRR---GPKAKLVELPDVGHAPTFMHAPQIEVARQFL 297 +E+ RR P+ ++ + D GH +H Q E Q + Sbjct: 253 LKREEIDRRIACIPRVQVEMIADAGH---MLHHDQPEQLAQLI 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,004,927 Number of extensions: 43562 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 3 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)