BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0027.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2... 609 e-176 gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 184 9e-48 gi|300311090|ref|YP_003775182.1| allophanate hydrolase subunit 2... 163 2e-41 >gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 325 Score = 609 bits (1571), Expect = e-176, Method: Compositional matrix adjust. Identities = 311/323 (96%), Positives = 318/323 (98%) Query: 1 MTIKVKQPGLATSVQDGGREGYYHLGIPPSGALDQYALRSANLLVGNHATTSALECSLLG 60 MTIKVKQPGLATSVQDGGREGYYHLGIPPSGALDQYALRSANLLVGNHA+TSALECSLLG Sbjct: 1 MTIKVKQPGLATSVQDGGREGYYHLGIPPSGALDQYALRSANLLVGNHASTSALECSLLG 60 Query: 61 PQLEFRSDALVAVTGARMQPRVDGSERPLDTAFMVRSGQVLSFDYPKAGARSYLAIAGGI 120 PQLEFRSDALVAVTGARMQPRVDGSERPLDTAFMVRSGQVLSFDYPKAGARSYLAIAGGI Sbjct: 61 PQLEFRSDALVAVTGARMQPRVDGSERPLDTAFMVRSGQVLSFDYPKAGARSYLAIAGGI 120 Query: 121 DVPVVLESRSTYALGALGGWQGRKLAKEDELPLGRASPKAVEGRALPLSLVPVFDRETTL 180 DVPVVLESRSTYALGALGGWQGRKLAK+DELPLGRASPKA+EGR LPLSLVPV+DRE TL Sbjct: 121 DVPVVLESRSTYALGALGGWQGRKLAKDDELPLGRASPKAIEGRTLPLSLVPVYDREATL 180 Query: 181 RVVPGLYIHRLTEEAVERFFADTWTVGSEADRIGYRFKGGQAMQFKPREQPFGAGSDPSN 240 RVVPGLYIHRLT+EAVERFF DTWTVGSEADRIGYRFKGGQAMQF+PREQPFGAGSDPSN Sbjct: 181 RVVPGLYIHRLTDEAVERFFDDTWTVGSEADRIGYRFKGGQAMQFRPREQPFGAGSDPSN 240 Query: 241 IVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISADLDLIGQLQPNHKAQFVRV 300 IVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISADLDLIGQLQPNHKAQFVRV Sbjct: 241 IVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISADLDLIGQLQPNHKAQFVRV 300 Query: 301 TLEQALEARRQYQQRFQRLSSLL 323 TLEQALEARRQYQQR QRL+S L Sbjct: 301 TLEQALEARRQYQQRIQRLASFL 323 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 184 bits (467), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 123/310 (39%), Positives = 168/310 (54%), Gaps = 9/310 (2%) Query: 2 TIKVKQPGLATSVQDG-GREGYYHLGIPPSGALDQYALRSANLLVGNHATTSALECSLLG 60 T++V PG T+VQD GR GY+ +G+PPSG +D+ ALR N L+GN + LE ++ G Sbjct: 454 TVEVLAPGTQTTVQDHPGRIGYWAVGVPPSGPMDERALRLGNRLLGNAEEAAGLEVTMSG 513 Query: 61 PQLEFRSDALVAVTGARMQPRVDGSERPLDTAFMVRSGQVLSFD-YPKAGARSYLAIAGG 119 P L F + A+V VTGA + +DG +P+ T + +G L AGARSYLA+ GG Sbjct: 514 PILRFNTAAMVVVTGAVIPVLLDGVAQPMATVLHIAAGSTLKLGAISGAGARSYLAVRGG 573 Query: 120 IDVPVVLESRSTYALGALGGWQGRKLAKEDELPLGRASPKAVE--GRALPLSLVPVFDRE 177 + VP L SRST+ LG GG GR LA D L L + A+ G+ LP +L P + Sbjct: 574 VQVPTYLGSRSTFTLGQFGGHAGRALASGDILHLAPLAADAMTQAGQTLPAALYPALEAV 633 Query: 178 TTLRVVPGLY--IHRLTEEAVERFFADTWTVGSEADRIGYRFKGGQAMQFKPREQPFGAG 235 +RV+ G + T +E FFA W V + R G R G + R+ AG Sbjct: 634 RQVRVIYGPHGAPEYFTPAYMETFFATDWEVHFNSSRTGVRLIGPKPEWV--RDSGGEAG 691 Query: 236 SDPSNIVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISADLDLIGQLQPNHKA 295 PSNI D Y +G++ G + P++L D S GG+ TVI ADL IGQL+ K Sbjct: 692 LHPSNIHDNPYAVGAVDFTGDM-PVILGPDGPSLGGFVCPVTVIEADLWQIGQLKAGDKV 750 Query: 296 QFVRVTLEQA 305 +FV V + A Sbjct: 751 RFVPVEVNTA 760 >gi|300311090|ref|YP_003775182.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 344 Score = 163 bits (412), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 120/334 (35%), Positives = 171/334 (51%), Gaps = 32/334 (9%) Query: 3 IKVKQPGLATSVQDGGREGYYHLGIPPSGALDQYALRSANLLVGNHATTSALECSLLGPQ 62 I + QPG+ SVQD GR G+ LGI P GAL+ AL SANLLVGN + LE ++ + Sbjct: 2 IDIIQPGMLASVQDMGRYGHRRLGIHPGGALNTLALASANLLVGNPVDAAGLEITMGVAE 61 Query: 63 LEFRSDALVAVTGARMQPRVDGSERPLDTAFMVRSGQVLSFDY---------PKAGARSY 113 L F +A+ G + +DG + VR+G +L P+ R+Y Sbjct: 62 LRFTRATRIALGGDDIGATLDGKPIAACWSIPVRAGSILKLSLAAADSPEQAPRHSMRTY 121 Query: 114 LAIAGGIDVPVVLESRSTYALGALGGWQGRKLAKEDELPLG--RASPKAVEGRAL----- 166 LA+AGGIDVP+VL SRST GG QGR L + D+LP+G +A+ AV G A Sbjct: 122 LAVAGGIDVPLVLGSRSTDLKAGFGGHQGRALKRGDKLPIGDAQAAAAAVHGPAFGVRSP 181 Query: 167 ---PLSL-VPVFDRETTLRVVPGLYIHRLTEEAVERFFADTWTVGSEADRIGYRFKGGQA 222 L+L + TLRV+P + ++ A E ++DTW V ++R+GYR +G + Sbjct: 182 RWSGLALRADEHSQHVTLRVLPAPEFDQFSKSAREALWSDTWRVTPNSNRMGYRLEGPEL 241 Query: 223 MQFKPREQPFGAGSDPSNIVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISAD 282 + + R+ G P G IQ+P +PI+L+ DA + GGY IG +I AD Sbjct: 242 KRKQGRDM-LSHGVVP----------GVIQVPPSGQPIILMGDAQTTGGYPKIGVIIGAD 290 Query: 283 LDLIGQLQPNHKAQFVRVTLEQALEARRQYQQRF 316 L + Q N + + AL A + QQR+ Sbjct: 291 LWKLAQAPLNATLRLEPCEMADALAAAEE-QQRY 323 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,080,894 Number of extensions: 48452 Number of successful extensions: 142 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 3 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)