BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0021.p (686 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313407|ref|YP_003777499.1| ring-opening enzyme protein [He... 1273 0.0 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 100 3e-22 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 96 8e-21 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 94 5e-20 gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 91 4e-19 >gi|300313407|ref|YP_003777499.1| ring-opening enzyme protein [Herbaspirillum seropedicae SmR1] Length = 686 Score = 1273 bits (3293), Expect = 0.0, Method: Compositional matrix adjust. Identities = 640/684 (93%), Positives = 666/684 (97%) Query: 1 MSARPPLLQSFIAGRWFGESPAQALHSAVNGHTVASTHQESIDFGEALEHARKKGLPALM 60 M+A PPLLQSFIAGRW G+ P+QALHSAVNG VASTH+ESIDFGEALEHARK GLPALM Sbjct: 1 MTAHPPLLQSFIAGRWLGQRPSQALHSAVNGSAVASTHEESIDFGEALEHARKTGLPALM 60 Query: 61 QLDFQQRAAILKALAKYLVEHKETLYAVSAHTGATRTDSWIDIEGGSGTLFTYASIGTNE 120 LDFQQRAAILKALAKYL+EHKE LYAVSAHTGATR DSWIDIEGGSGTLFTYASIG NE Sbjct: 61 ALDFQQRAAILKALAKYLMEHKEVLYAVSAHTGATRADSWIDIEGGSGTLFTYASIGANE 120 Query: 121 FPSSNLVHEGPAISLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMP 180 FPSSNLVHEGPA+ LGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMP Sbjct: 121 FPSSNLVHEGPAMRLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMP 180 Query: 181 CIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLDRLQAQDVVTFTGSADTA 240 CIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLL+RLQAQDVVTFTGSADTA Sbjct: 181 CIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQDVVTFTGSADTA 240 Query: 241 AKLRAHPNLIANSIPFNAEADSLNCAILGPDVNADDEEFDLFVKEVAREMTTKAGQKCTA 300 AKLRAHPNLIANSIPFNAEADSLNCAILGPD+ ADDEEFDLFVKEVAREMTTKAGQKCTA Sbjct: 241 AKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMTTKAGQKCTA 300 Query: 301 IRRAIVPRQHLDAISEKLAARLSKTVIGDPALESVRMGPLASRAQQRDVEERVALLKQSA 360 IRRAIVPR+H+DA++E+LAARLSK V+GDPALE+VRMGPLASRAQQRDVEERVALL+ SA Sbjct: 301 IRRAIVPRRHVDAVAERLAARLSKIVVGDPALETVRMGPLASRAQQRDVEERVALLRHSA 360 Query: 361 ELILGAGSSFQPQGEGVGEGAFFAPTLLLARDTSAGSPVHEVEAFGPVSTLMPYDDLDEA 420 +LILGAGS+F PQG+GVGEGAFFAPTLLLARDT+AGSPVHEVEAFGPVSTLMPYDDLDEA Sbjct: 361 DLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGPVSTLMPYDDLDEA 420 Query: 421 LALAARGRGSLVASLVTRSPSVAARAIPVAAAWHGRLLVLDREAAPESTGHGSPLPQLKH 480 LALAARGRGSLVASLVTR+P+VAARAIPVAAAWHGRLLVLDREAA ESTGHGSPLPQLKH Sbjct: 421 LALAARGRGSLVASLVTRTPAVAARAIPVAAAWHGRLLVLDREAAAESTGHGSPLPQLKH 480 Query: 481 GGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGAKVRESDIHPFRHYFE 540 GGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGA+VRES IHPFRHYFE Sbjct: 481 GGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGARVRESGIHPFRHYFE 540 Query: 541 DLQIGDSLATHRRTVTEADIVNFGCLSGDHFYMHFDDISARQSPFGKRIAHGYFVLSAAA 600 DLQIGDSL THRRTVTEADIVNFGCLSGDHFYMHFD+I+ARQSPFGKRIAHGYFVLSAAA Sbjct: 541 DLQIGDSLTTHRRTVTEADIVNFGCLSGDHFYMHFDEIAARQSPFGKRIAHGYFVLSAAA 600 Query: 601 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD 660 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD Sbjct: 601 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD 660 Query: 661 VEVSNQDGELVASYDILTLVAKKP 684 VEVSNQ+GELVASYDILTLVAKKP Sbjct: 661 VEVSNQEGELVASYDILTLVAKKP 684 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 100 bits (250), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 133/512 (25%), Positives = 210/512 (41%), Gaps = 61/512 (11%) Query: 7 LLQSFIAGRWFGESPAQALH--SAVNGHTV-ASTHQESIDFGEALEHARKKGLPALMQLD 63 + Q FIA RW + +++ +G A + D +A+ AR A +L Sbjct: 1 MKQHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLS 60 Query: 64 FQQRAAILKALAKYLVEHKETLYAVSAHTGA-----TRTDSWIDIEGGSGTLFTYASIGT 118 +R+ +L LA L +H+E L + R D+ + F + + Sbjct: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVR-----YFEFYAGAA 115 Query: 119 NEFPSSNLVHEGPAISLGKQGRFAGTHILVPRG--GVAVHINAFNFPVWGMLEKFAPTFL 176 ++ + ++ AG +L R GV HI +N+P+ Sbjct: 116 DKLHGDTIPYQ------------AGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALA 163 Query: 177 AGMPCIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIG---STGDLLDRLQAQDVVTF 233 AG C+VKPA L + G PEG+L +V G GDLL + + ++F Sbjct: 164 AGNTCVVKPAEDACLSILRVAELAAEVGF-PEGALNIVTGYGHEAGDLLVKHPDVNHISF 222 Query: 234 TGSADTAAKLRAHPNLIANSIPFNAEADSLNCAILGPDVNADDEEFDLFVKEVAREMTTK 293 TGS + L+ + + +L P + +D + + + V + Sbjct: 223 TGSPNVG-------KLVVRAAAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQN 275 Query: 294 AGQKCTAIRRAIVPRQHLDAISEKLAARLSKTVIGDPALESVRMGPLASRAQQRDVEERV 353 AGQ C+A R +V R + + E+L+ S+ +G ++ + GPL + QQ ERV Sbjct: 276 AGQTCSAGSRLLVQRGAYEQVLERLSGLFSQLQVGSSQMD-LDCGPLIRKTQQ----ERV 330 Query: 354 ALLKQSAELILGAGSSFQPQGE---GVGEGAFF-APTLLLARDTSAGSPVHEVEAFGPVS 409 A A+ G + QG+ GV G F+ AP LL RD + + E FGPV Sbjct: 331 AGFLSDAQR---DGIALAAQGKIAAGVPTGGFYQAPALL--RDVPPRHRLAQEEVFGPVL 385 Query: 410 TLMPYDDLDEALALAARGRGSLVASLVTRSPSVAARAIPVAAAWHGRLLVLDREAAPEST 469 MP++D A+ALA LVAS+ TR R + +A A + ++ A Sbjct: 386 AAMPFEDEAHAIALANGTDYGLVASVWTRD---GGRQMRLARAIRSGQVFINNYGA---- 438 Query: 470 GHGSPLP--QLKHGGPGRAGGGEELGGSRAVK 499 G G LP +K G GR G E L G +K Sbjct: 439 GGGVELPFGGVKASGHGREKGFEALYGFTVLK 470 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 96.3 bits (238), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 76/277 (27%), Positives = 131/277 (47%), Gaps = 18/277 (6%) Query: 152 GVAVHINAFNFPVWGMLEKFAPTFLAGMPCIVKPATATSYLAEAAVRLIQQSGLLPEGSL 211 GV I +N+P++ + K P AG ++KP+ + A ++ ++G+ P G Sbjct: 149 GVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLFAEIVHEAGV-PAGVF 207 Query: 212 QLVIGSTGDLLDRLQAQ---DVVTFTGSADTAAKLRAHPNLIANSIPFNAEADSLNCAIL 268 LV GS ++ L A D+++ TGS L+A + + + Sbjct: 208 NLVNGSGAEVGAALSAHPQVDMISITGSTRAGV-------LVAQAAAVTVKRVAQELGGK 260 Query: 269 GPDVNADDEEFDLFVKEVAREMTTKAGQKCTAIRRAIVPRQHLDAISEKLAARLSKTVIG 328 P++ D + + + GQ C+A R IVPR L + E A L++ +G Sbjct: 261 SPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALATLAQMKVG 320 Query: 329 DPALESVRMGPLASRAQQRDVEERV-ALLKQSAELILGAGSSFQPQGEGVGEGAFFAPTL 387 DP + GPLA+RAQ V++ + A L+ A L+ AG +P +G+ +G + PT+ Sbjct: 321 DPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLL--AGGPGRP--DGLAQGYYARPTI 376 Query: 388 LLARDTSAGSPVHEVEAFGPVSTLMPYDDLDEALALA 424 + + + E FGPV ++PYD ++EA+A+A Sbjct: 377 F--SEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIA 411 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 93.6 bits (231), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 115/452 (25%), Positives = 188/452 (41%), Gaps = 54/452 (11%) Query: 5 PPLL--QSFIAGRWFGESPAQ--ALHSAVNGHTVASTH-QESIDFGEALEHARKKGLPAL 59 P LL Q+F+ G+W + A+H+ NG + + + A+E A PA Sbjct: 7 PALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAA-NAAWPAW 65 Query: 60 MQLDFQQRAAILKALAKYLVEHKETLYAV-SAHTGATRTDSWIDIEGGSGTLFTYASIGT 118 + ++R+AIL+ + ++ + + L + +A G ++ +I + + +A G Sbjct: 66 KKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGK 125 Query: 119 NEF--------PSSNLVHEGPAISLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEK 170 + PS+ +V AI GV I +NFP + K Sbjct: 126 RTYGDTIPSPSPSNRIVVIKEAI------------------GVCAAITPWNFPAAMITRK 167 Query: 171 FAPTFLAGMPCIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLDRLQAQDV 230 P AG P ++KPA AT + A A L +++G+ P G +V G+ + + + + Sbjct: 168 AGPALAAGCPMVLKPAEATPFSALALAVLAERAGI-PAGVFSVVTGTPKGIGGEMTSNPI 226 Query: 231 V---TFTGSADTAAKLRAHPNLIANSIPFNAEADSLNCAILGPDVNADDEEFDLFVKEVA 287 V +FTGS L A+SI + SL P + DD + D V+ Sbjct: 227 VRKISFTGSTGVGKLLMQQS---ASSI----KKLSLELGGNAPFIVFDDADLDAAVEGAI 279 Query: 288 REMTTKAGQKCTAIRRAIVPRQHLDAISEKLAARLSKTVIGDPALESVRMGPLASRAQQR 347 AGQ C R V DA + KL + K +G E V GPL + + Sbjct: 280 ASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQ 339 Query: 348 DVEERVA-LLKQSAELILGAGSSFQPQGEGVGEGAFFAPTLLLARDTSAGSPVHEVEAFG 406 VE+ VA + + A ++LG + +G +FF PT+L D + V E FG Sbjct: 340 KVEQHVADAVAKGARVLLGG------KRHALGH-SFFEPTVL--ADVTPAMQVAREETFG 390 Query: 407 PVSTLMPYDDLDEALALAARGRGSLVASLVTR 438 P++ L + +E LALA L + +R Sbjct: 391 PMAPLFRFKTDEEVLALANDTEFGLASYFYSR 422 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 90.5 bits (223), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 108/360 (30%), Positives = 160/360 (44%), Gaps = 42/360 (11%) Query: 152 GVAVHINAFNFPVWGMLEKFAPTFLAGMPCIVKPATATSYLAEAAV-RLIQQSGLLPEGS 210 GV I+ FNFP+ L AP AG +VKP T + R+ +Q+GL P G Sbjct: 147 GVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVSGGFMLARIFEQAGL-PAGL 205 Query: 211 LQLVIG--STGDLLDRLQAQDVVTFTGSADTAAKLRAHPNLIANSIPFNAEADSLNCAIL 268 L ++ G TG+ L ++ FTGS + I + + SL Sbjct: 206 LHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRR-------IGELAGRHLKKLSLELGGA 258 Query: 269 GPDVNADDEEFDLFVKEVAREMTTKAGQKCTAIRRAIVPRQHLDAISEKLAARLSKTVIG 328 V +D + D+ A GQ C A R +V + + ++LA + +G Sbjct: 259 NALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEGLLQRLADKARHLPVG 318 Query: 329 DPALESVRMGPLASRAQQRDVEERVALLKQS----AELILGAGSSFQPQGEGVGEGAFFA 384 D A V +GP+ Q + V AL+++S A+L+ G G E F+ Sbjct: 319 DGASGQVALGPMIDERQLQRVH---ALVQESVAAGAQLVAG----------GRYEQLFYQ 365 Query: 385 PTLLLARDTSAGSPVHEVEAFGPVSTLMPYDDLDEALALAARGRGSLVASLVTRSPSVAA 444 PT+L G V E E FGPV++++ +D+ ++A+ALA R G L A ++ SPSV Sbjct: 366 PTVL--GGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVL--SPSV-G 420 Query: 445 RAIPVAAAW-HGRLLVLDREAAPESTGHGSPLPQLKHGGPGRAGGGEELGGSRAVKHYLQ 503 RA+ VAA G + V D+ E +P GGPG AG G +GG + Y Q Sbjct: 421 RAMAVAARLRQGMVHVNDQTVNDECV---NPF-----GGPGIAGNGASVGGPADWEEYTQ 472 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,111,801 Number of extensions: 91178 Number of successful extensions: 364 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 341 Number of HSP's successfully gapped: 19 Length of query: 686 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 594 Effective length of database: 1,184,250 Effective search space: 703444500 Effective search space used: 703444500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)