BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0005.p (450 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309354|ref|YP_003773446.1| 4-aminobutyrate aminotransferas... 430 e-121 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 152 5e-38 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 147 1e-36 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 129 7e-31 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 120 2e-28 >gi|300309354|ref|YP_003773446.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 430 bits (1106), Expect = e-121, Method: Compositional matrix adjust. Identities = 222/444 (50%), Positives = 290/444 (65%), Gaps = 8/444 (1%) Query: 1 MSTPTDLTMVNAYIPGR-ADVGPVTGALIERRNALLGPAYRLMYEHPLHIVRGEGVWLID 59 M+ + +NA+ P + P T ALIERR G L Y P+H V GVWL D Sbjct: 1 MTHADGMLALNAFNPQSGSTTAPATRALIERRRNTFGDGVPLFYRDPVHFVSASGVWLRD 60 Query: 60 PQGRRYLDVYNNVTSLGHCHPAVTAAICEQVQTLATNTRYLHDTILELAERLLATVPETS 119 G YLD YNNV S+GHCHP V A+ +Q TL T+TRYL D + AERLL T+P Sbjct: 61 QDGLEYLDAYNNVPSVGHCHPRVVEAVSKQAATLNTHTRYLSDVVTSYAERLLGTLPR-E 119 Query: 120 LAHLMLTCTGSEANDLAYRIAKVRTGGTGVIVTDTAYHGITDAVSQFSPSLGSSVNLGPH 179 L+ ++ T +G+E+ DLA R+A++ TGG+G IVT AYHG + AV++ +P+ G V +G + Sbjct: 120 LSKVLFTSSGTESVDLAMRLARLYTGGSGFIVTRFAYHGHSTAVAEITPAFGPGVPIGIN 179 Query: 180 VRLVPAPRAYHAPGQD--LGEKFTRDVQAAIADLQRHGIRPAALIVDSLFTSDGILPGPA 237 VRLV AP Y QD +G KF +DV AAIAD++RHGIR A LI+D++ +SDG+ P Sbjct: 180 VRLVDAPDGYR---QDAPVGIKFAQDVAAAIADMERHGIRFAGLILDTVCSSDGLFVDPP 236 Query: 238 GFLTGAVQAIKEAGGLFIADEVQPGFGRTGEHMWGFQRHGIAPDIVTMGKPMGNGQPVAG 297 GFL A+ +K+AGG+FIADEVQPGFGRTGE MWGF+RH I PDIV MGKPMGNG P+A Sbjct: 237 GFLKDAIATVKKAGGVFIADEVQPGFGRTGEGMWGFERHRIVPDIVVMGKPMGNGMPIAA 296 Query: 298 ILATADVLQEFGQRSRYFNTFAGNTVSCAAALAVLKTIERESLIPHAREVGGVLLDGIRA 357 + AD++Q F +RS YFNTF GNTV CAAA AVL IE E LI +A+E G L G+ Sbjct: 297 TVTRADIMQAFTERSGYFNTFGGNTVCCAAASAVLDVIESEGLINNAKETGASLKLGLEQ 356 Query: 358 LAVRHEAIGDVRGAGMFVGVELVSDRAQRTPDRTLTSEVVNRMRQKGVLLSACAMGHNVL 417 L +H+ IGDVR G++ VE V +T D + VVN +RQK +L+S C N+L Sbjct: 357 LKGQHQCIGDVRAIGLYAAVEFVLPDT-KTADTDMALHVVNTLRQKRILISTCGPAGNIL 415 Query: 418 KIRPPLVLTGEQAGMVIDALDESL 441 KIRPPL + + +++ A+D+ L Sbjct: 416 KIRPPLQFSRQHCDLLVTAIDDVL 439 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 152 bits (384), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 118/395 (29%), Positives = 190/395 (48%), Gaps = 20/395 (5%) Query: 46 PLHIVRGEGVWLIDPQGRRYLDVYNNV--TSLGHCHPAVTAAICEQVQTLATNTRYLHDT 103 P+ + + GV DP G ++D+ V +S+G C+P V AI +Q ++L + Sbjct: 48 PIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNSSK 107 Query: 104 ILELAERLLATVPETSLAHLM--LTCTGSEANDLAYRIAKVRTGGTGVIVTDTAYHGITD 161 ELA ++ +P+ + T GS+A + A + AK TG +I YHGI + Sbjct: 108 RTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWN 167 Query: 162 AVSQFSPSLGSSVNLGPHVRLV---PAPRAYHAPGQDLGEKFTRDVQAAIADLQRH---- 214 A + + GP + V P P AY P D K + D + Sbjct: 168 ASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFP-FDTSHKSAEQIAGEYVDYLLNTPYT 226 Query: 215 -GIRPAALIVDSLFTSDGILPGPAGFLTGAVQAIKEAGGLFIADEVQPGFGRTGEHMWGF 273 AA+IV+ + G +P FL +A +G L I DEVQ G GRTG+ MW Sbjct: 227 AADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGK-MWAV 285 Query: 274 QRHGIAPDIVTMGKPMGNGQPVAGILATADVLQEFGQRSRYFNTFAGNTVSCAAALAVLK 333 + G+ PD++T GK +G P+AG++ +D+ + S+ NTFA N++S A AL + Sbjct: 286 EHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQP-NTFAANSISAAVALTNIS 344 Query: 334 TIE--RESLIPHAREVGGVLLDGIRALAVRHEAIGDVRGAGMFVGVELVSDRAQRTP-DR 390 ++ R L+ A +G + IR+ +G+VRG G+ +G+ELV +R R P R Sbjct: 345 ILQDPRLDLVNRAHTLGLEAQERIRSF--NSPWVGEVRGRGLMIGIELVENRETREPLSR 402 Query: 391 TLTSEVVNRMRQKGVLLSACAMGHNVLKIRPPLVL 425 ++++ + GVL+ C NV+++ P L + Sbjct: 403 EKLGKLMDYVVGHGVLMIPCGRYTNVMRVMPSLTI 437 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 147 bits (372), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 124/413 (30%), Positives = 192/413 (46%), Gaps = 29/413 (7%) Query: 27 LIERRNALLGPAYRLMYEHPLHIVRGEGVWLIDPQGRRYLDVYNNVTSL--GHCHPAVTA 84 L +R+NA +M + + R L D +GRR++D + L GH HP + Sbjct: 10 LQQRKNAATPRGVGVMCD--FYAERAANAELWDVEGRRFIDFAAGIAVLNTGHRHPKLLD 67 Query: 85 AICEQVQTLATNTRYL---HDTILELAERLLATVPETSLAHLMLTCTGSEANDLAYRIAK 141 A+ Q+ T+T Y + + +ELAER+ P TG+EA + A +IA+ Sbjct: 68 AMRAQMDKF-THTAYQIVPYASYVELAERINRLTPGNYPKKTAFFSTGAEAVENAIKIAR 126 Query: 142 VRTGGTGVIVTDTAYHGITDAVSQFSPSLGS-SVNLGPH---VRLVPAPRAYHA-PGQDL 196 TG GVI +HG T + + + GP V P P A H +D Sbjct: 127 AHTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLGFGPFPGDVFHAPYPSALHGITSEDA 186 Query: 197 GEK----FTRDVQAAIADLQRHGIRPAALIVDSLFTSDGILPGPAGFLTGAVQAIKEAGG 252 E F D++A R AA+I++ + G PA F+ G E G Sbjct: 187 LEAVKGLFKSDIEAK---------RVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGI 237 Query: 253 LFIADEVQPGFGRTGEHMWGFQRHGIAPDIVTMGKPMGNGQPVAGILATADVLQEFGQRS 312 L IADEVQ G+GRTG+ ++ + + + PD++TM K + G P++ + A+++ + Sbjct: 238 LLIADEVQSGYGRTGK-LFAMEHYDVLPDLMTMAKSLAGGMPLSAVNGRAEIM-DAPAPG 295 Query: 313 RYFNTFAGNTVSCAAALAVLKTIERESLIPHAREVGGVLLDGIRALAVRHEAIGDVRGAG 372 T+AGN ++ A+ALAVL +E E L+ + +G L + ++ L I +VRG G Sbjct: 296 GLGGTYAGNPLAIASALAVLDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVG 355 Query: 373 MFVGVELVSDRAQRTPDRTLTSEVVNRMRQKGVLLSACAMGHNVLKIRPPLVL 425 V VE +D A PD T +V G+LL C NV++ PL + Sbjct: 356 AMVAVEF-ADPATGKPDAEYTKKVQQHALNNGLLLLTCGSYGNVIRFLFPLTI 407 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 129 bits (323), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 128/449 (28%), Positives = 203/449 (45%), Gaps = 40/449 (8%) Query: 26 ALIERRNALLGPAYRLMYEH----PLHIVRGEGVWLIDPQGRRYLDVYNNV--TSLGHCH 79 A ++RR+ L+ P L EH PL + G+G +L D Q ++ LD ++ + + G+ Sbjct: 7 AALDRRH-LIHPVTSLR-EHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQ 64 Query: 80 PAVTAAICEQVQTLATNTRYLH---DTILELAERLLATVPETSLAHLMLTCTGSEANDLA 136 +V A EQ+Q L T Y H + + LA++L+ P SL H+ T GS+A D A Sbjct: 65 ESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISP-ASLQHVYFTLGGSDAVDAA 123 Query: 137 ------YRIAKVRTGGTGVIVTDTAYHGITDAVSQFS--PSLGSSVNLG-PHVRLVPAPR 187 Y + + G I YHG + + + P+ NL P +P+P Sbjct: 124 IRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPY 183 Query: 188 AYHAPGQDLGEKFTRDVQA---AIADLQRHGIRPAALIVDSLFTSDGILPGPAGFLTGAV 244 Y + Q VQA +A+L + AA + + S G++ P G+L Sbjct: 184 PYRSELQGDEAIIAASVQALRDKVAELGADNV--AAFFCEPIQGSGGVVVPPKGWLKAMQ 241 Query: 245 QAIKEAGGLFIADEVQPGFGRTGEHMWGFQRHGIAPDIVTMGKPMGNGQ-PVAGILATAD 303 A E LF+ DEV GFGRTG M+ + PD++TM K + G P+ ++ + Sbjct: 242 LACNELDILFVVDEVITGFGRTGP-MFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDR 300 Query: 304 VLQEFGQRSR------YFNTFAGNTVSCAAALAVLKTIERESLIPHAREVGGVLLDGIRA 357 V + + T++ + VS A AL VL+ E L+ + R + V G+RA Sbjct: 301 VYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRA 360 Query: 358 LAVRHEAIGDVRGAGMFVGVELVSD---RAQRTPDRTLTSEVVNRMRQKGVLLSACAMGH 414 L + H +GD R G+ +ELV+D +A+ P+ L + + G++ A G Sbjct: 361 L-LAHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFR--AFGD 417 Query: 415 NVLKIRPPLVLTGEQAGMVIDALDESLTE 443 N+L P L T E ++ L L E Sbjct: 418 NILGFAPALCYTQEDFALMFARLKAILDE 446 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 122/431 (28%), Positives = 204/431 (47%), Gaps = 51/431 (11%) Query: 50 VRGEGVWLIDPQGRRYLDVYNN--VTSLGHCHPAVTAAICEQVQTLA-TNTRYLHDTILE 106 V GEG+ LID G+RY+D V+ LGH HP V AI +QV LA +T + E Sbjct: 17 VAGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSFFTTAPAE 76 Query: 107 LAERLLATVPETSLAHLMLTCTGSEANDLAYRIAKVRTGGTG------VIVTDTAYHGIT 160 +LA SL H+ GSEA + A ++A+ G +I +YHG T Sbjct: 77 ELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQRRHIIARRQSYHGNT 136 Query: 161 DAV----------SQFSPSLGSSVNLGPHVRLVPAPRAYHAPGQDLGEKFTRDVQAAIAD 210 F P L + ++ P Y + GE + VQ + Sbjct: 137 LGALAIGGNAWRREMFMPMLIEAHHVSP---------CYAYRNRADGESDVQYVQRLADE 187 Query: 211 LQRHGI-----RPAALIVDSLF-TSDGILPGPAGFLTGAVQAIKEAGG-LFIADEVQPGF 263 L++ + + A + +++ + G +P P G ++A+ + G L I DEV G Sbjct: 188 LEQKILSLGADQVIAFVAETVVGATAGAVP-PVGDYFRKIRAVCDKYGVLLILDEVMSGM 246 Query: 264 GRTGEHMWGFQRHGIAPDIVTMGKPMGNG-QPVAGILATADVLQEFGQRSRYF---NTFA 319 GRTG +++ + G+ PDIV + K +G G QP+ ++ + + + S +F +T+ Sbjct: 247 GRTG-YLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGHTYI 305 Query: 320 GNTVSCAAALAVLKTIERESLIPHAREVGGVLLDGIRALAVRHEAIGDVRGAGMFVGVEL 379 G+ +CAAA+AV KTI+ E L+ + R+ G L +R +GD+RG G+FVGVEL Sbjct: 306 GHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVGVEL 365 Query: 380 VSDRAQR---TPDRTLTSEVVNRMRQKGVLLSACA------MGHNVLKIRPPLVLTGEQA 430 V++R+ + +PD + V ++G+L+ G ++L + PP + + Sbjct: 366 VAERSSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHIL-LAPPFIASSNDI 424 Query: 431 GMVIDALDESL 441 ++ L +++ Sbjct: 425 SEIVQRLSDAV 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,546,820 Number of extensions: 71351 Number of successful extensions: 253 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 222 Number of HSP's successfully gapped: 9 Length of query: 450 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 362 Effective length of database: 1,203,190 Effective search space: 435554780 Effective search space used: 435554780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)