BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0188.p (233 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 445 e-126 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 125 2e-30 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 123 1e-29 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 120 1e-28 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 107 7e-25 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 445 bits (1144), Expect = e-126, Method: Compositional matrix adjust. Identities = 221/234 (94%), Positives = 227/234 (97%), Gaps = 1/234 (0%) Query: 1 VAMHLELREAIATLQADTSVRVLLLTGAGRGFCAGQDLGDRA-REGDGAVDLGESIDKYY 59 VAMHLELR+AIATLQAD SVRVLLLTGAGRGFCAGQDLGDRA + GD VDLGESIDKYY Sbjct: 30 VAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAGQDLGDRAVKPGDDGVDLGESIDKYY 89 Query: 60 GPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGT 119 GPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGT Sbjct: 90 GPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGT 149 Query: 120 YFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDKLMEEAYAMATHFARAPTKG 179 YFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDD+LMEEA+AMA HFARAPTKG Sbjct: 150 YFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDQLMEEAHAMAAHFARAPTKG 209 Query: 180 LAATKATLYASPANTLPEQLDLERDTMRALGHSRDYREGVSAFLEKRAPQFTGQ 233 LAATKATLYASPA+TLPEQLDLERDTMRALG SRDYREGV+AFLEKRAPQFTGQ Sbjct: 210 LAATKATLYASPAHTLPEQLDLERDTMRALGRSRDYREGVTAFLEKRAPQFTGQ 263 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 125 bits (315), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 86/225 (38%), Positives = 122/225 (54%), Gaps = 4/225 (1%) Query: 6 ELREAIATLQADTSVRVLLLTGAGRGFCAGQDLGDRAREGDGAVDLGESIDKYYGPLVKS 65 EL EA+ A + +++TG+ + F AG D+ A V GE I + + ++ Sbjct: 35 ELGEALLAFDAQEEIGCIIITGSEKAFAAGADISAMAGYDYMDVFKGEFITRNW----ET 90 Query: 66 LRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRL 125 LR + PVI AV G A G G L + CD ++AA +A F + KLG++P GGT LPR Sbjct: 91 LRRIRKPVIAAVAGYALGGGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRA 150 Query: 126 VGTARAMGMALLGEKISAEQAERWGLIWKCVDDDKLMEEAYAMATHFARAPTKGLAATKA 185 V A+AM +AL G + AE+AER GL+ + V DKL+EEA A A A P + K Sbjct: 151 VSKAKAMDLALTGRMMDAEEAERAGLVSRIVAADKLLEEAMAAAIIIAEMPRQVAMMVKD 210 Query: 186 TLYASPANTLPEQLDLERDTMRALGHSRDYREGVSAFLEKRAPQF 230 ++ + TL E + ER + + D +EG+ AFLEKR P F Sbjct: 211 SVNRAYETTLSEGMKYERALFYSCFATEDQKEGMKAFLEKRQPVF 255 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 123 bits (308), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 84/230 (36%), Positives = 122/230 (53%), Gaps = 8/230 (3%) Query: 7 LREAIATL---QADTSVRVLLLTGAGRGFCAGQDLGDRAREGDGAVDLGESIDKYYGPLV 63 LRE +A L + D SVRV++++G + F AG DL + + A L + +Y+ Sbjct: 38 LREIVAALARAEEDDSVRVVVISGNEKAFAAGADLNEMIHKDAIATQL-DVRAQYW---- 92 Query: 64 KSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLP 123 +++ P P++ AVNG A GAG L + DI +AAR A + +G +P GGT L Sbjct: 93 RAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQPEINVGTLPGAGGTQRLI 152 Query: 124 RLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDKLMEEAYAMATHFARAPTKGLAAT 183 R VG AM M L GE ISA+QA + GL+ + VDDD +E A+A A+ + Sbjct: 153 RTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTLALAHSIAQKSPLAVRLA 212 Query: 184 KATLYASPANTLPEQLDLERDTMRALGHSRDYREGVSAFLEKRAPQFTGQ 233 K + S L L ER + + S D +EG++AF EKRA F+G+ Sbjct: 213 KEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEKRAAVFSGR 262 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 83/229 (36%), Positives = 116/229 (50%), Gaps = 4/229 (1%) Query: 6 ELREAIATLQADTSVRVLLLTGAG-RGFCAGQDLGDRAREGDGAVDLGESIDKYYGPLVK 64 +L + L AD + L++TG G + F AG DL A DG + + + +G + Sbjct: 35 DLTRLVKELNADRDIYTLVVTGEGEKFFSAGADLKLFA---DGDKAMAREMARRFGEAFE 91 Query: 65 SLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPR 124 +L A I A+NG A G G LACDI +A A +GL+P GGT LP Sbjct: 92 TLSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMALPEAAVGLLPCAGGTQNLPW 151 Query: 125 LVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDKLMEEAYAMATHFARAPTKGLAATK 184 LVG A M L GE+++AE A R GL+ + V + E A A+A + +AA+K Sbjct: 152 LVGEGWAKRMILCGERVNAETALRIGLVEEVVPRGQARERALALAQQAEKQSPSSVAASK 211 Query: 185 ATLYASPANTLPEQLDLERDTMRALGHSRDYREGVSAFLEKRAPQFTGQ 233 + + N L L ER+ L ++D REGV AFLEKR+PQ+ Q Sbjct: 212 QLIQGARHNPLGTILQTERELFVDLFDTQDQREGVQAFLEKRSPQWKNQ 260 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 107 bits (268), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 72/224 (32%), Positives = 108/224 (48%), Gaps = 3/224 (1%) Query: 7 LREAIATLQADTSVRVLLLTGAGRGFCAGQDLGDRAREGDGAVDLGESIDKYYGPLVKSL 66 L+ + +++D++VR ++L G+ FCAG DL R + E++ L++SL Sbjct: 39 LQAQLDDIKSDSTVRCVILAANGKAFCAGHDL--RQMQATPQQTYYEALFHRCSHLMQSL 96 Query: 67 RALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLV 126 R LP PVI V G+A AG L CD+ +AA SA F +GL T L R + Sbjct: 97 RELPVPVIAKVQGLATAAGCQLVATCDLAIAAESARFAVSGINVGLFCSTPAVA-LTRNL 155 Query: 127 GTARAMGMALLGEKISAEQAERWGLIWKCVDDDKLMEEAYAMATHFARAPTKGLAATKAT 186 RA M + G+ I A A WGLI + V + +L A+A P +A KA Sbjct: 156 PIKRAFEMLVTGKFIDAATAAAWGLINQAVPEQELDSATQALAREICSKPAISIARGKAM 215 Query: 187 LYASPANTLPEQLDLERDTMRALGHSRDYREGVSAFLEKRAPQF 230 +Y LP+ + M + +EG++AFL+KR P + Sbjct: 216 VYRQQMMALPDAYAHAAEVMACNIMDPEAQEGITAFLDKRKPNW 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 737,483 Number of extensions: 30571 Number of successful extensions: 106 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 9 Length of query: 233 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 151 Effective length of database: 1,231,600 Effective search space: 185971600 Effective search space used: 185971600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)