BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0187.p (521 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase... 753 0.0 gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase... 103 2e-23 gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase... 86 1e-17 >gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 512 Score = 753 bits (1945), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/512 (72%), Positives = 424/512 (82%) Query: 10 MSAAESPHALPATARVAVIGSGTMGAGIAQVAAVAGHPVQLYDSRDGAAAKAIADIRASL 69 M+ + PHAL +A VAVIGSGTMGAGIAQVAAVAGHPV LYDSR+GAA KAI I +L Sbjct: 1 MNNRKYPHALSRSALVAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICTNL 60 Query: 70 DKLANKGKITHEQAQGASDRLSVAHALQEMRDARLVVEAIVEQLAAKQELFGKLEAIVDA 129 DKL +K K+T AQ AS R+SVA +L+E+ A LV+EAIVEQLAAK ELFGKLEAIV Sbjct: 61 DKLVSKDKLTPALAQAASQRISVAQSLEELHQADLVIEAIVEQLAAKHELFGKLEAIVST 120 Query: 130 QCILASNTSSISITQIAATLRHPERLAGMHFFNPVPMMALVEIIRGVCSDATVVQCLHDT 189 +CILA+NTSS+SITQIAA L+HPER+ GMHFFNPVPMMALVEII G+ SDA + QCLHDT Sbjct: 121 RCILATNTSSLSITQIAAGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLHDT 180 Query: 190 ALAWGKVPVVAASTPGFIVNRLARPYYAEALRVLNERGAQPATIDAILRESGGFRMGAFE 249 ALAWGK PV+A STPGFIVNRLARPYYAE LR+L+E GAQPATIDA++RE+GGFRMG FE Sbjct: 181 ALAWGKTPVMARSTPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFRMGPFE 240 Query: 250 LMDLIGHDVNFAVTQSVFQAYFNDPRFTPSLIQQELVHAGFLGRKSGRGFYRYGEAPETP 309 LMDLIGHDVNFAVTQSVF AYFNDPRFTPSLIQQELVHAG+LGRKSGRGFYRYGEA P Sbjct: 241 LMDLIGHDVNFAVTQSVFHAYFNDPRFTPSLIQQELVHAGYLGRKSGRGFYRYGEAAGAP 300 Query: 310 QPVTVPAFDTPTVPLQLFGTHPLVEVIRARYQGRVEYCEARADHLLMKAGPGRLYMTGGR 369 +P T + PT QL+G HPLVE+I RY G+V+ AR D LLMK L +T GR Sbjct: 301 RPETERDYPKPTATPQLYGDHPLVEMIHTRYPGQVQRHPARPDQLLMKVNAACLCITDGR 360 Query: 370 SATQQSYDYNMPDVVLVDLALDYATAPRLALTRADQCRDRAWEAVAGVLQHCGFALSPLQ 429 SAT +++++ PD+VLVDLALDYA A RLAL +ADQCR + + VAGVLQ CG LSP+Q Sbjct: 361 SATLRAWEHQEPDLVLVDLALDYADATRLALAKADQCRPQRYAEVAGVLQACGLDLSPMQ 420 Query: 430 DVPGMVVMRTVCMLINEAADAVSQGVCSKQDADLAMQKGVNYPRGPLAWADAIGVDTVAN 489 DVPGM+VMRTVCML+NEAADAV+QGVC+ DAD+AM+KGVNYPRGPLAWA+ IG+DTV Sbjct: 421 DVPGMMVMRTVCMLVNEAADAVNQGVCNAADADMAMRKGVNYPRGPLAWANEIGIDTVNQ 480 Query: 490 VLRQLSATYGEDRYRVAPLLQRLQASGRKFHE 521 VL L ATYGEDRYR++PLLQRL ASGRKFHE Sbjct: 481 VLHHLCATYGEDRYRISPLLQRLYASGRKFHE 512 >gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 805 Score = 103 bits (258), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 94/297 (31%), Positives = 143/297 (48%), Gaps = 30/297 (10%) Query: 24 RVAVIGSGTMGAGIAQVAAVAGHPVQLYD------SRDGAAAKAIADIRA-SLDKLANKG 76 +VAV+G+G MGA IA A PV L+D ++G +AI +++ S L NK Sbjct: 18 KVAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSPAPLGNKD 77 Query: 77 KITHEQAQGASDRLSVAHALQEMRDARLVVEAIVEQLAAKQELFGKLEAIVDAQCILASN 136 Q D L+V + L++EAI E++ K +L+ K+ + + I ASN Sbjct: 78 DAALIQVANYEDDLAV------LEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFASN 131 Query: 137 TSSISITQIA----ATLRHPERLAGMHFFNPVPMMALVEIIRGVCSDATVVQCLH---DT 189 TS +SI ++ A L+ R G+HFFNP M LVE+I ++ ++ L T Sbjct: 132 TSGLSINALSEGFDAGLK--ARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTT 189 Query: 190 ALAWGKVPVVAASTPGFIVNRLARPYYAEALRVLNERGAQPATIDAIL-RESGGFRMGAF 248 L GK V A TP F+ NR+ + + G +D + + G + G F Sbjct: 190 TL--GKGVVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTF 247 Query: 249 ELMDLIGHDVNFAVTQSVFQAYFNDPRF----TPSLIQQELVHAGFLGRKSGRGFYR 301 D++G D V +++ +DP F TP L+ + LV G LG+KSG GFY+ Sbjct: 248 RTADVVGLDTMGHVIRTMQDNLKDDPFFSSYATPPLLAK-LVEQGALGQKSGAGFYK 303 >gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 323 Score = 85.5 bits (210), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 74/238 (31%), Positives = 110/238 (46%), Gaps = 9/238 (3%) Query: 23 ARVAVIGSGTMGAGIAQVAAVAGHPVQLYDSRDGAAAKAIADIRASLDKLANKGKITHEQ 82 ARVAV+G+G +GA A G V D A K A I L G H Sbjct: 16 ARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIARHWPSLQALGLAEH-- 73 Query: 83 AQGASDRLSVAHALQE-MRDARLVVEAIVEQLAAKQELFGKLEAIVDAQCILASNTSSIS 141 + +RL L+ + A V E E+ K +LF +++A + AQ +LAS++S + Sbjct: 74 --ASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQVVLASSSSGLL 131 Query: 142 ITQIAATLRHPERLAGMHFFNPVPMMALVEIIRGVCSDATVVQCLHDTALAWGKVPV-VA 200 ++++ A RHP+R+ H FNP ++ LVE+I G + VQ D A GK P+ V Sbjct: 132 MSKVQAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFYAAIGKKPIHVR 191 Query: 201 ASTPGFIVNRLARPYYAEALRVLNERGAQPATIDAILRESGGFR---MGAFELMDLIG 255 G I NRL + EA ++ + A ID + G R MG + + + G Sbjct: 192 KEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPYLNLHMSG 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,583,591 Number of extensions: 66019 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 243 Number of HSP's successfully gapped: 3 Length of query: 521 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 431 Effective length of database: 1,193,720 Effective search space: 514493320 Effective search space used: 514493320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)