BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0183.p (732 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herba... 742 0.0 gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [H... 625 e-180 gi|300313420|ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbas... 564 e-161 gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [H... 274 2e-74 gi|300309598|ref|YP_003773690.1| acetyl-CoA acyltransferase [Her... 274 2e-74 >gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 742 bits (1915), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/400 (95%), Positives = 391/400 (97%) Query: 333 MEALICDAIRTPFGRYGGALGAVRADDLAAAPIRSLMERNPGVDWRCVDDILYGCANQAG 392 MEALICDAIRTPFGRYGGALGAVRADDLAAAPIRSLMERNPGVDW V+DILYGCANQAG Sbjct: 1 MEALICDAIRTPFGRYGGALGAVRADDLAAAPIRSLMERNPGVDWSRVEDILYGCANQAG 60 Query: 393 EDNRNVARMAGLLAGLPIEIPGTTLNRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMT 452 EDNRNVARMAGLLAGLPI +PG+T+NRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMT Sbjct: 61 EDNRNVARMAGLLAGLPIAVPGSTVNRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMT 120 Query: 453 RAPFVMGKAESAFSRSAAIFDTTIGWRFVNPLMKAQYGIDSMPETAENVATDFNINRADQ 512 RAPFVMGKAESAF+RSAAIFDTTIGWRFVNPLMKAQYGIDSMPETAENVATDF INRADQ Sbjct: 121 RAPFVMGKAESAFARSAAIFDTTIGWRFVNPLMKAQYGIDSMPETAENVATDFQINRADQ 180 Query: 513 DAFALRSQQRWAAAQEASFFAGEIAPLAIPQKKGDPLIVTTDEHPRPDTTLATLAKLKGV 572 DAFALRSQQRWAAAQ A FFAGEIAPL IPQKKGDPL+VTTDEHPRPDTTLATLAKLKGV Sbjct: 181 DAFALRSQQRWAAAQAAGFFAGEIAPLTIPQKKGDPLVVTTDEHPRPDTTLATLAKLKGV 240 Query: 573 VRPDGTVTAGNASGVNDGACALLLASPKAADLYRLKPRARVLGMATAGVAPRIMGFGPAP 632 VRPDGTVTAGNASGVNDGACALLLASPKAADLYRLKPRARVLGMATAGVAPRIMGFGPAP Sbjct: 241 VRPDGTVTAGNASGVNDGACALLLASPKAADLYRLKPRARVLGMATAGVAPRIMGFGPAP 300 Query: 633 AARKVLAQVGLTLAQMDVIELNEAFAAQGLAVTRDLGLPDDAAHVNPNGGAIAIGHPLGA 692 A RKVLAQVGLTLAQMDVIELNEAFAAQGLAV RDLGLPDDAAHVNPNGGAIAIGHPLGA Sbjct: 301 AVRKVLAQVGLTLAQMDVIELNEAFAAQGLAVMRDLGLPDDAAHVNPNGGAIAIGHPLGA 360 Query: 693 SGARLVTTALNQLERTGGRYALCTMCIGVGQGIALVIERV 732 SGARLVTTA+NQLER+GGRYALCTMCIGVGQGIALVIERV Sbjct: 361 SGARLVTTAINQLERSGGRYALCTMCIGVGQGIALVIERV 400 >gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 625 bits (1613), Expect = e-180, Method: Compositional matrix adjust. Identities = 309/399 (77%), Positives = 350/399 (87%), Gaps = 1/399 (0%) Query: 334 EALICDAIRTPFGRYGGALGAVRADDLAAAPIRSLMERNPGVDWRCVDDILYGCANQAGE 393 + ICDAIRTP GRYGGAL VRADDL A P+R+LMERNP VDW+ VDD++YGCANQAGE Sbjct: 3 DVFICDAIRTPIGRYGGALKDVRADDLGAVPLRALMERNPQVDWKAVDDVIYGCANQAGE 62 Query: 394 DNRNVARMAGLLAGLPIEIPGTTLNRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMTR 453 DNRNVARM+ LLAGLP E+PG+T+NRLCGS +DA+G AARAIKSGE QLMIAGGVESMTR Sbjct: 63 DNRNVARMSLLLAGLPQEVPGSTINRLCGSGMDALGTAARAIKSGETQLMIAGGVESMTR 122 Query: 454 APFVMGKAESAFSRSAAIFDTTIGWRFVNPLMKAQYGIDSMPETAENVATDFNINRADQD 513 APFVMGKA+SAFSR AAI DTTIGWRFVN LMK +YG+D+MPETAENVA DF ++RADQD Sbjct: 123 APFVMGKADSAFSRQAAIQDTTIGWRFVNALMKQKYGVDAMPETAENVAVDFKVSRADQD 182 Query: 514 AFALRSQQRWAAAQEASFFAGEIAPLAIPQKKGDPLIVTTDEHPRPDTTLATLAKLKGVV 573 FA+RSQ + AAAQ A EI P+ IPQKKGDP+ VT DEHPR T++ LA+LKGVV Sbjct: 183 QFAVRSQAKAAAAQANGTLAQEIVPVVIPQKKGDPITVTQDEHPRA-TSMEALARLKGVV 241 Query: 574 RPDGTVTAGNASGVNDGACALLLASPKAADLYRLKPRARVLGMATAGVAPRIMGFGPAPA 633 +PDG+VTAGNASGVNDGACALLLAS +A + Y+LKPRAR+LGMATAGVAPRIMG GPAPA Sbjct: 242 KPDGSVTAGNASGVNDGACALLLASAEAVEKYQLKPRARILGMATAGVAPRIMGMGPAPA 301 Query: 634 ARKVLAQVGLTLAQMDVIELNEAFAAQGLAVTRDLGLPDDAAHVNPNGGAIAIGHPLGAS 693 ++KVLAQ+G+T+ QMDVIELNEAFA+QGLAV R+LG+ DD A VNPNGGAIA+GHPLG S Sbjct: 302 SKKVLAQLGMTIDQMDVIELNEAFASQGLAVLRELGVADDDARVNPNGGAIALGHPLGMS 361 Query: 694 GARLVTTALNQLERTGGRYALCTMCIGVGQGIALVIERV 732 GARLVTTA QLERTGGRYALCTMCIGVGQGIA+VIERV Sbjct: 362 GARLVTTATYQLERTGGRYALCTMCIGVGQGIAMVIERV 400 >gi|300313420|ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 564 bits (1453), Expect = e-161, Method: Compositional matrix adjust. Identities = 270/280 (96%), Positives = 276/280 (98%) Query: 1 VQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDFKPSIIMVTPSYMLNV 60 VQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDFKPSIIMVTPSYMLNV Sbjct: 136 VQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDFKPSIIMVTPSYMLNV 195 Query: 61 IEEFQRQGLDAAASSLKVGIFGAEPWTDAMRREIQQRSGIDAVDIYGLSEVMGPGVASEC 120 IEEFQRQG+D A+SSLKVGIFGAEPWTDAMRREIQQR+G+DAVDIYGLSEVMGPGVASEC Sbjct: 196 IEEFQRQGMDPASSSLKVGIFGAEPWTDAMRREIQQRAGLDAVDIYGLSEVMGPGVASEC 255 Query: 121 IESKDGPVIWEDHFYPEIIDPDTGEVLPDGEEGELVFTSLTKEALPMIRYRTRDLTRLLP 180 IESKDGPVIWEDHFYPEIIDPDTGEVLPDG EGELVFTSL+KEALPMIRYRTRDLTRLLP Sbjct: 256 IESKDGPVIWEDHFYPEIIDPDTGEVLPDGVEGELVFTSLSKEALPMIRYRTRDLTRLLP 315 Query: 181 PTSRAMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQYQLQIERHGHMDSMC 240 PTSR+MRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQYQLQIERHGHMDSM Sbjct: 316 PTSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQYQLQIERHGHMDSMS 375 Query: 241 VFAELREDASNEHVEALEKELQHSIKTYVGISTKVTVVPA 280 VFAELRED SNEHVEALEKELQHSIKTYVGISTKVTVVPA Sbjct: 376 VFAELREDVSNEHVEALEKELQHSIKTYVGISTKVTVVPA 415 >gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 391 Score = 274 bits (701), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 164/402 (40%), Positives = 242/402 (60%), Gaps = 17/402 (4%) Query: 334 EALICDAIRTPFGRYGGALGAVRADDLAAAPIRSLMERNPGVDWRCVDDILYGCANQAGE 393 + +I A RT G++GGAL + A DL A I++L+ + G+ + +++ G AG Sbjct: 3 DVVIVAAQRTAIGKFGGALSKIAAADLGAQVIKALLAKT-GIKPEAISEVILGQVLTAGL 61 Query: 394 DNRNVARMAGLLAGLPIEIPGTTLNRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMTR 453 +N AR + + +GLP +P + ++CGS L AV +AA+AI+ G+ Q++IAGG E+M+ Sbjct: 62 -GQNPARQSVIKSGLPDMVPAFVVGKVCGSGLKAVQLAAQAIRCGDAQIVIAGGQENMSA 120 Query: 454 APFVMGKAESAFSR-SAAIFDTTIG---WRFVNPLMKAQYGIDSMPETAENVATDFNINR 509 +P V+ + F A + DT I W N QY M TAENVA F I+R Sbjct: 121 SPHVLNNSRDGFRMGDAKLTDTMIVDGLWDVYN-----QY---HMGITAENVAKKFEISR 172 Query: 510 ADQDAFALRSQQRWAAAQEASFFAGEIAPLAIPQKKGDPLIVTTDEHPRPDTTLATLAKL 569 +QDAFA SQ + AAQ+A F EI P+ I KK + ++ TDE + T +LA L Sbjct: 173 EEQDAFAAASQNKAEAAQKAGKFKDEIVPIEIKGKK-ETVVFDTDEFVKHGVTAESLATL 231 Query: 570 KGVVRPDGTVTAGNASGVNDGACALLLASPKAADLYRLKPRARVLGMATAGVAPRIMGFG 629 + GTVTAGNASG+NDGA A+++ S K A L A++ ++AG+ P IMG G Sbjct: 232 RPAFDKAGTVTAGNASGINDGAAAVVVTSAKLAAELGLPVLAKIKAYSSAGLDPSIMGMG 291 Query: 630 PAPAARKVLAQVGLTLAQMDVIELNEAFAAQGLAVTRDLGLPDDAAHVNPNGGAIAIGHP 689 P PA++ L + G T +D++E+NEAFAAQ +AV + +G D + +N NGGAIA+GHP Sbjct: 292 PVPASQLTLKKAGWTPQDLDLMEINEAFAAQAIAVNKQMGW--DTSKINVNGGAIALGHP 349 Query: 690 LGASGARLVTTALNQLERTGGRYALCTMCIGVGQGIALVIER 731 +GASG R++ T L+++ R + L ++CIG G G+AL +ER Sbjct: 350 IGASGCRVLVTLLHEMVRRDAKKGLASLCIGGGMGVALAVER 391 >gi|300309598|ref|YP_003773690.1| acetyl-CoA acyltransferase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 274 bits (700), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 180/414 (43%), Positives = 231/414 (55%), Gaps = 29/414 (7%) Query: 330 KTPMEALICDAIRTPFGRY-GGALGAVRADDLAAAPIRSLMERNPGVDWRCVDDILYGCA 388 K +A I A RTP G+ G R DDL I+S + + PG+D + ++D + GC+ Sbjct: 3 KQLQDAYIVAATRTPIGKAPRGMFKNTRPDDLLVRAIQSAVAQVPGLDPKLIEDAIVGCS 62 Query: 389 NQAGEDNRNVARMAGLLAGLPIEIPGTTLNRLCGSSLDAVGMAARAIKSGEVQLMIAGGV 448 G N+AR A LLAGLP I G T+NR C S + A+ MAA I+ GE +MIA G Sbjct: 63 FPEGAQGLNMARNAVLLAGLPNTIGGVTINRYCASGITAIAMAADRIRVGEADVMIAAGA 122 Query: 449 ESMTRAPFVMGKAESAFSRSAAIFDTTIGWR-FVNPLMKAQYGIDSMPETAENVATDFNI 507 ESM+ P +MG F +I F + + YG M TAE VA + + Sbjct: 123 ESMSMVP-MMG------------FHPSININAFKDENVGMAYG---MGLTAEKVAQQWKV 166 Query: 508 NRADQDAFALRSQQRWAAAQEASFFAGEIAPLAIPQK-----KGDPLI----VTTDEHPR 558 +R QD F+L S Q+ AAQEA FA E+ I ++ G+ + V+ DE PR Sbjct: 167 SREAQDEFSLASHQKAIAAQEAGEFADEMTSFEIVERFPNLATGEIDVKTRTVSLDEGPR 226 Query: 559 PDTTLATLAKLKGVVRPDGTVTAGNASGVNDGACALLLASPKAADLYRLKPRARVLGMAT 618 D+ LA LAKLK V G+VTAGN+S +DGA AL++ S K + L P AR + A Sbjct: 227 ADSNLAALAKLKPVFAAKGSVTAGNSSQTSDGAGALIIVSEKILKQFNLTPLARFVSFAV 286 Query: 619 AGVAPRIMGFGPAPAARKVLAQVGLTLAQMDVIELNEAFAAQGLAVTRDLGLPDDAAHVN 678 GV P IMG GP A L GLT Q+D IELNEAFAAQ LAV DLGL D + VN Sbjct: 287 RGVPPEIMGIGPKEAIPAALKAGGLTQDQIDWIELNEAFAAQALAVIGDLGL--DPSKVN 344 Query: 679 PNGGAIAIGHPLGASGARLVTTALNQLERTGGRYALCTMCIGVGQGIALVIERV 732 P GGAIA+GHPLGA+GA T ++ L R +Y + TMC+G G G A + ERV Sbjct: 345 PMGGAIALGHPLGATGAIRAATTIHALRRKNLKYGMVTMCVGTGMGAAGIFERV 398 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,455,848 Number of extensions: 111648 Number of successful extensions: 408 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 390 Number of HSP's successfully gapped: 8 Length of query: 732 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 640 Effective length of database: 1,184,250 Effective search space: 757920000 Effective search space used: 757920000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)