BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0175.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313429|ref|YP_003777521.1| zinc protease-like signal pepti... 599 e-172 gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 87 2e-18 gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 59 4e-10 >gi|300313429|ref|YP_003777521.1| zinc protease-like signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 438 Score = 599 bits (1544), Expect = e-172, Method: Compositional matrix adjust. Identities = 297/324 (91%), Positives = 308/324 (95%) Query: 1 VSLRSLSSPAERDAALGLLGRMLAHPAFPQAGLERDRALAIANLKEELTKPETIAEKAFM 60 VSLR+LSSPAER+AAL LL RMLAHP+FPQA LERDRALAIAN+KEELTKPE IAEKAFM Sbjct: 114 VSLRTLSSPAEREAALTLLARMLAHPSFPQASLERDRALAIANIKEELTKPEVIAEKAFM 173 Query: 61 EAAYGSHPYAVDASEASLQAITREDLLAFHRAHYVANRAVIALIGDITEAQARSIASALT 120 AAYGSHPYA+DASEAS+QAITREDL AFHRAHYVANRAVIALIGDI QAR+IASALT Sbjct: 174 HAAYGSHPYAMDASEASMQAITREDLQAFHRAHYVANRAVIALIGDINLEQARAIASALT 233 Query: 121 RELPQGSALPALPQVAAPKGVERRIAHPASQSHILIGAPAIQRGDNDFFALTVGNYVLGG 180 RELPQG+ALPALP V APKG E RIAHPASQSHILIGAPAIQRGD DFFALTVGNYVLGG Sbjct: 234 RELPQGAALPALPPVVAPKGSEERIAHPASQSHILIGAPAIQRGDPDFFALTVGNYVLGG 293 Query: 181 GGFVSRLTDEVREKRGLSYSVYSYFSPLAQPGPFQIGLQTKKEQTAEALRVTRATLDKFL 240 GGFVSRLTDEVREKRGLSYSVYS FSPLAQPGPFQIGLQTKKEQTAEALRVTR TLDKF+ Sbjct: 294 GGFVSRLTDEVREKRGLSYSVYSGFSPLAQPGPFQIGLQTKKEQTAEALRVTRVTLDKFM 353 Query: 241 QEGPTAAELKAAKDNLAGGFALRIDSNAKLLENLSVIGFYGLPLDYLDHWIERIRAVSVQ 300 QEGPTAAELKAAKDNLAGGFALRIDSNAKLLENLSVIGFYGLPLDYLDHWIERIRAVSVQ Sbjct: 354 QEGPTAAELKAAKDNLAGGFALRIDSNAKLLENLSVIGFYGLPLDYLDHWIERIRAVSVQ 413 Query: 301 DVRAAFRKHVHPEELSTVIVGEAA 324 DVRAAFRKHVHPEELST+IVGE+A Sbjct: 414 DVRAAFRKHVHPEELSTIIVGESA 437 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 83/334 (24%), Positives = 144/334 (43%), Gaps = 47/334 (14%) Query: 15 ALGLLGRMLAHPAFPQAGLERDRALAIANLKEELTKPETIAEKAFMEAAYGSHP------ 68 A+ L+G + HP A E+ + +A L+ P+T A +A + + +P Sbjct: 604 AIALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTRAGEA-LSVHFDHYPAGDWRA 662 Query: 69 -YAVDASEASLQAITREDLLAFHRAHYVANRAVIALIGDITEAQARSIAS----ALTREL 123 ++ A+++A+T E + FHR Y A++ +A++GD AQARS T Sbjct: 663 AQTLEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFDPAQARSAIDQAFGGWTSAA 722 Query: 124 PQGSALPALPQVAAPKGVERRIAHPASQSHILIGAPAIQRGDND--FFALTVGNYVLGGG 181 P L + A + + + P ++ + I+ D D + AL V N++LGG Sbjct: 723 PYARVLQTWADIPAKRLL---VDTPDKENGVYFARQNIRLSDTDPDYPALAVANFLLGGS 779 Query: 182 GFVSRLTDEVREKRGLSYSVYS--YFSPLAQPGPFQIGLQTKKEQTAEALRVTRATLDKF 239 SRL D VR++ GLSY +YS + L G I + + R + Sbjct: 780 SLKSRLADRVRQQDGLSYGIYSGLQVAALTDAGHLTIYAIAAPQNLDKVDAAVREEIALA 839 Query: 240 LQEGPTAAELKAAK--------------DNLAGGFALRIDSNAKLLENLSVIGFYGLPLD 285 L++G + EL+ AK DN+A + L ++ + L + Sbjct: 840 LKDGFSEEELQRAKSGILQQRNQQRAGNDNVAATWTLLMNQDRNFLWQQEI--------- 890 Query: 286 YLDHWIERIRAVSVQDVRAAFRKHVHPEELSTVI 319 ++ ++++ V AA RK+V P ++TVI Sbjct: 891 -----DRKLASLTLAQVNAALRKYVDPARMTTVI 919 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 64/261 (24%), Positives = 123/261 (47%), Gaps = 15/261 (5%) Query: 50 KPETIAEKAFMEAAYGSHPY--AVDASEASLQAITREDLLAFHRAHYVANRAVIALIGDI 107 +P + +A AA+ +HPY V LQ ++ +D+ A++R Y N A + + GD+ Sbjct: 161 QPMGLLNEALNAAAWTAHPYHHPVVGWMDDLQHMSVQDIAAWYRQWYAPNNATLVVAGDV 220 Query: 108 TEAQARSIASALTRELPQGSALPALPQVAAPKGVERR---IAHPASQSHILIG--APAIQ 162 + ++A ++P LPA P+ + R + PA ++++ PA++ Sbjct: 221 DAQRVLALARKYFGKIP-ARRLPAGKPQNEPQQLGMRRVTVKAPAENPYVVMAWKTPALR 279 Query: 163 R--GDNDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSYFSPLAQPGPFQIGLQT 220 + D D +AL V + VL G +RL+ + G + +V + +S +A+ GP L+ Sbjct: 280 QVEQDQDVYALDVLSAVLDGYDN-ARLSASLVRTGGKATAVGASYSGVAR-GPVLFTLEG 337 Query: 221 KKEQ---TAEALRVTRATLDKFLQEGPTAAELKAAKDNLAGGFALRIDSNAKLLENLSVI 277 + TA+ + R +++ +EG + EL+ K L + DS + ++ Sbjct: 338 SPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQLIASQVYKRDSVFGQAMEIGMM 397 Query: 278 GFYGLPLDYLDHWIERIRAVS 298 GL +D IER+++V+ Sbjct: 398 ETAGLGQQNIDRIIERLKSVT 418 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,023,756 Number of extensions: 43684 Number of successful extensions: 180 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 177 Number of HSP's successfully gapped: 3 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)