BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0172.p (464 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 796 0.0 gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 148 1e-36 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 796 bits (2056), Expect = 0.0, Method: Compositional matrix adjust. Identities = 403/464 (86%), Positives = 430/464 (92%), Gaps = 5/464 (1%) Query: 1 MKSKIRALLATALLSLLAATAAQVRAEPAIFNAHXEFVLANGMKVVVKEDRRAPTAVQMV 60 MK KIRALLA+ALL L A++ +AEPA +FVL NGMKV+VKEDRRAPTAVQMV Sbjct: 1 MKLKIRALLASALLGLFASSPMLAQAEPA-----RQFVLGNGMKVIVKEDRRAPTAVQMV 55 Query: 61 WYKVGSIDELNGTTGVSHALEHMMFKGTKNHKVGEFSRLVAELGGQENAFTANDFTAYFQ 120 WYKVG IDE+NG TGVSHALEHMMFKGT+NHKVGEFSRLVAELGGQENAFTANDFTAYFQ Sbjct: 56 WYKVGGIDEVNGLTGVSHALEHMMFKGTRNHKVGEFSRLVAELGGQENAFTANDFTAYFQ 115 Query: 121 QIEKSHLEKVMALEADRMANLQFDAKEFAKEIRVIMEERRWRTDDQPMGLLNEALNAAAW 180 QIEKSHLEKVMALEADRMANLQFDA EFAKEIRVIMEERRWRTDDQPMGLLNEALNAAAW Sbjct: 116 QIEKSHLEKVMALEADRMANLQFDAAEFAKEIRVIMEERRWRTDDQPMGLLNEALNAAAW 175 Query: 181 TAHPYHHPVVGWMDDLQHMTVQDIASWYRQWYAPNNATLVVAGDVDAQRVLALAKKYFGK 240 TAHPYHHPVVGWMDDLQHM+VQDIA+WYRQWYAPNNATLVVAGDVDAQRVLALA+KYFGK Sbjct: 176 TAHPYHHPVVGWMDDLQHMSVQDIAAWYRQWYAPNNATLVVAGDVDAQRVLALARKYFGK 235 Query: 241 IPSHPVPQGKPQNEPEQLGLRRVTVKAPAENPYVVMAWKVPALRNVEADQEAYALDVLSA 300 IP+ +P GKPQNEP+QLG+RRVTVKAPAENPYVVMAWK PALR VE DQ+ YALDVLSA Sbjct: 236 IPARRLPAGKPQNEPQQLGMRRVTVKAPAENPYVVMAWKTPALRQVEQDQDVYALDVLSA 295 Query: 301 VLDGYDNARLTANLIRTASQATAVGASYSGVARGPVLFTLEGSPANGVSTEQLEGLLRAE 360 VLDGYDNARL+A+L+RT +ATAVGASYSGVARGPVLFTLEGSPA GV+T QLEGLLR E Sbjct: 296 VLDGYDNARLSASLVRTGGKATAVGASYSGVARGPVLFTLEGSPAEGVTTAQLEGLLRGE 355 Query: 361 VQRIASEGVSEQELQRVKTQLIASQVYKRDSVFGQAMEIGMMETTGLGQKNIDRIIDRLK 420 V+RIA EGVSEQELQRVKTQLIASQVYKRDSVFGQAMEIGMMET GLGQ+NIDRII+RLK Sbjct: 356 VERIAREGVSEQELQRVKTQLIASQVYKRDSVFGQAMEIGMMETAGLGQQNIDRIIERLK 415 Query: 421 SVTAAQVQAVAQKYFKDETLTVATLVPLPLNGKKPAAPPPGLLH 464 SVTAAQVQAVAQ+YF+D+TLTVATLVPLPL GKKPA PP GLLH Sbjct: 416 SVTAAQVQAVAQQYFQDDTLTVATLVPLPLAGKKPAPPPAGLLH 459 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 148 bits (373), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 113/436 (25%), Positives = 209/436 (47%), Gaps = 31/436 (7%) Query: 36 EFVLANGMKVVVKEDRRAPTAVQMVWYKVGSIDELNGTTGVSHALEHMMFKGTKNHKVGE 95 E+ ANG K+++ D PT + Y VGS E G TG++H LEH+MFKG+ H Sbjct: 62 EYRFANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPRHP--S 119 Query: 96 FSRLVAELGGQENAFTANDFTAYFQQIEKS--HLEKVMALEADRMANLQFDAKEFAKEIR 153 + ++ G N T D T Y++ + S +L +A+EADRM N + K+ E+ Sbjct: 120 IPQDFSKRGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLNSKIARKDLDSEMT 179 Query: 154 VIMEERRWRTDDQPMGLLNEALNAAAWTAHPYHHPVVGWMDDLQHMTVQDIASWYRQWYA 213 V+ E + P ++ + + + A+ H Y +G D++++ ++++ ++YR +Y Sbjct: 180 VVRNEFE-AGETSPTRVMLKRMQSVAYDWHAYGRNTIGARSDIENVRIENLQAFYRTYYQ 238 Query: 214 PNNATLVVAGDVDAQRVLALAKKYFGKIPSHPVPQGKPQN--------EPEQLGLRRVTV 265 P+NA L++AG DA +VL + FG++P KP+ EP Q G R+ + Sbjct: 239 PDNAVLLIAGKFDAAQVLQWVDQSFGRLP-------KPKRTLPAFWTVEPTQDGERQFVI 291 Query: 266 KAPAENPYVVMAWKVPALRNVEADQEAYALDVLSAVLDGYDNARLTANLIRTASQATAVG 325 + + V +A+K+P+ + +A +A D+L+ +G RL L+ T Sbjct: 292 RRRGDQQLVALAYKMPSALHPDATALGFAADILTDTPNG----RLHKALVETGMATEVYA 347 Query: 326 ASYSGVARGPVLFTLEGSPANGVSTEQLEGLLRAEVQRIASEGVSEQELQRVKTQLIASQ 385 G+A P L + G + + + + V+ +++ + +E+ R+ + A+ Sbjct: 348 MPLEGMA--PGLQMMVAKVKVGGDLDAVRQAMISAVESFSTQPPTPEEVARLHRE--AAN 403 Query: 386 VYKRDSVFGQAMEIGMMETTGLGQKNIDRI-IDRLKSVTAAQVQAVAQKYFKDETLTVAT 444 ++ Q + + M G + I DR++ +++A + A A +YF+ + TV Sbjct: 404 SFETLQNNPQQLAVAMSNAIARGDWRLLFIERDRMQRLSSADIAAAAGRYFRRDNRTVGL 463 Query: 445 LVP--LPLNGKKPAAP 458 +P P + P AP Sbjct: 464 YLPDDHPQRAEIPTAP 479 Score = 60.1 bits (144), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 94/405 (23%), Positives = 166/405 (40%), Gaps = 57/405 (14%) Query: 63 KVGSIDELNGTTGVSHALEHMMFKGT----KNHKVGEFSRLVAELGGQENAFTANDFTAY 118 + G L G V+ E M+ +GT + EF+RL ++ G + Y Sbjct: 545 RFGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFARL--KING----------SLY 592 Query: 119 FQQIEKSHLEKVMALEADRMANLQFDAKEFAK---EIRVIMEERRWRTDDQPMGLLNEAL 175 Q + +L +AL + + + DA EF + E +E R D + EAL Sbjct: 593 RFQTTRENLVPAIALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTRA----GEAL 648 Query: 176 NAAAWTAHPYHHPVVGW---------MDDLQHMTVQDIASWYRQWYAPNNATLVVAGDVD 226 + H H+P W + ++ +T++ + ++R++Y + L V GD D Sbjct: 649 -----SVHFDHYPAGDWRAAQTLEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFD 703 Query: 227 AQRVLALAKKYFGKIPSHPVPQGKPQNEPEQLGLRRVTVKAPAENPYVVMAWKVPALRNV 286 + + + FG S P + + +R+ V P + V A + +R Sbjct: 704 PAQARSAIDQAFGGWTS-AAPYARVLQTWADIPAKRLLVDTPDKENGVYFARQ--NIRLS 760 Query: 287 EADQEAYALDVLSAVLDGYDNARLTANLIRTASQATAVGASYSGVA------RGPVLFTL 340 + D + AL V + +L G A+ +R Q YSG+ G + Sbjct: 761 DTDPDYPALAVANFLLGGSSLKSRLADRVR--QQDGLSYGIYSGLQVAALTDAGHLTIYA 818 Query: 341 EGSPANGVSTEQLEGLLRAEVQRIASEGVSEQELQRVKTQLIASQVYKR---DSVFGQAM 397 +P N ++++ +R E+ +G SE+ELQR K+ ++ + +R D+V Sbjct: 819 IAAPQN---LDKVDAAVREEIALALKDGFSEEELQRAKSGILQQRNQQRAGNDNVAATWT 875 Query: 398 EIGMMETTGLGQKNIDRIIDRLKSVTAAQVQAVAQKYFKDETLTV 442 + + L Q+ IDR +L S+T AQV A +KY +T Sbjct: 876 LLMNQDRNFLWQQEIDR---KLASLTLAQVNAALRKYVDPARMTT 917 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,321,278 Number of extensions: 53019 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 3 Length of query: 464 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 375 Effective length of database: 1,198,455 Effective search space: 449420625 Effective search space used: 449420625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)