BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0154.p (150 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313448|ref|YP_003777540.1| integral membrane protein [Herb... 201 3e-53 >gi|300313448|ref|YP_003777540.1| integral membrane protein [Herbaspirillum seropedicae SmR1] Length = 156 Score = 201 bits (510), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 104/136 (76%), Positives = 116/136 (85%) Query: 15 WVFAVLCTVSLTVALGFTLRGAWIILVFSCIELVAVGAAMLVYARHATDREVLVLADHGL 74 WVFA+LCTVSLTVALGFTL GAWIILVFS IE AVGAAMLVYARHATDREV+ LAD GL Sbjct: 21 WVFAILCTVSLTVALGFTLHGAWIILVFSFIESTAVGAAMLVYARHATDREVVRLADEGL 80 Query: 75 EVEVIQGSRSRRSSLQLHGMRIAFPVGRQSLVALESRGQRVEIGRFMSLWQRRALVQELQ 134 VEV QGSRS RS+L GMRI +PVGRQ+LVALESRGQRVE+GRFM+ QRR +ELQ Sbjct: 81 VVEVTQGSRSSRSTLHWQGMRIDYPVGRQALVALESRGQRVEVGRFMNEGQRREFARELQ 140 Query: 135 QELAARGGGVAALSFL 150 +EL+AR G +AA+SFL Sbjct: 141 RELSARTGAMAAVSFL 156 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.334 0.142 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 390,282 Number of extensions: 12514 Number of successful extensions: 110 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 110 Number of HSP's successfully gapped: 1 Length of query: 150 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 73 Effective length of database: 1,255,275 Effective search space: 91635075 Effective search space used: 91635075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 126 (53.1 bits)