BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0142.p (346 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313460|ref|YP_003777552.1| branched-chain amino acid ABC t... 501 e-143 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 63 4e-11 gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 57 2e-09 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 56 4e-09 >gi|300313460|ref|YP_003777552.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 430 Score = 501 bits (1290), Expect = e-143, Method: Compositional matrix adjust. Identities = 245/257 (95%), Positives = 249/257 (96%), Gaps = 2/257 (0%) Query: 46 VRIGMIDGLSGPFANAGEAVVRNISYAIERVNARGGVNLPDGKHLLQLSTFDNKLGVEDS 105 +RIGMIDGLSGPFANAGEAVVRNISYAIERVNARGGV LPDGKHLLQLSTFDNKLGVEDS Sbjct: 34 IRIGMIDGLSGPFANAGEAVVRNISYAIERVNARGGVTLPDGKHLLQLSTFDNKLGVEDS 93 Query: 106 LVQFRQLTDERIPFLIEGNSSAVAAALIDAVNKHNQREPDRRVLFLNYSAVDPTLTNEKC 165 LVQFRQLTDERIPFLIEGNSSAVAAALIDAVNKHNQREPDRRVLFLNYSAVDPTLTNEKC Sbjct: 94 LVQFRQLTDERIPFLIEGNSSAVAAALIDAVNKHNQREPDRRVLFLNYSAVDPTLTNEKC 153 Query: 166 SFWHFRFDASADMRMQALTEAIKADPATKKVYLIGQDYSFGRQVAKAAREQITAKRPDIA 225 SFWHFRFDASADMRMQALTEAIKAD AT+KVYLIGQDYSFGRQVAKAAREQ+ AKRPDIA Sbjct: 154 SFWHFRFDASADMRMQALTEAIKADTATRKVYLIGQDYSFGRQVAKAAREQLAAKRPDIA 213 Query: 226 IVGDELHPIGKIKDFAPYIAKMRSAGADAVITGNWGNDLTLLVKAARDAGFKAKFYTFYA 285 IVGDELHPIGKIKDFAPYIAKMRSAGADAVITGNWGNDLTLLVKAARDAGFKAKFYTFYA Sbjct: 214 IVGDELHPIGKIKDFAPYIAKMRSAGADAVITGNWGNDLTLLVKAARDAGFKAKFYTFYA 273 Query: 286 NSLGAPAAPXLGTRGWG 302 NSLGAPAA +G G G Sbjct: 274 NSLGAPAA--IGEAGVG 288 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 62.8 bits (151), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 71/267 (26%), Positives = 125/267 (46%), Gaps = 24/267 (8%) Query: 13 LSSRLPLRMFPAALLALAAVSTPVCSVAAPLPPVRIGMIDGLSGPFANA-GEAVVRNISY 71 +S+ PLR AA A+A + + VRIG I +SGP+A+ G + I Sbjct: 1 MSTSTPLRRLAAAC-AIAGIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRM 59 Query: 72 AIERVNARGGVNLPDGKHLLQLSTFDNKLGVEDSLVQFRQLTDER-IPFLIEGNSSAVAA 130 A+ GG L GK + +S D++ + + + R+ DER + LI G +SA A Sbjct: 60 AVADF---GGTVL--GKPIEVISA-DHQNKADIAATRAREWADERGLDMLIGGVNSATAL 113 Query: 131 ALIDAVNKHNQREPDRRVLFLNYSAVDPTLTNEKCSFW--HFRFDASADMRMQALTEAIK 188 ++ N+ +++ +++N A LTNE+C+ + H+ +D A + A + IK Sbjct: 114 SM-------NKVMAEKKRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTA-SAVIK 165 Query: 189 ADPATKKVYLIGQDYSFGRQVAKAAREQITAKRPDIAIVGDELHPIGKIKDFAPYIAKMR 248 K + + DY+FG +A + A +VG HP + D + ++ + + Sbjct: 166 --QGGKSWFFLVADYAFGHSLANDTSAVVKASGG--TVVGSIKHP-PQSSDLSSFLLQAQ 220 Query: 249 SAGADAVITGNWGNDLTLLVKAARDAG 275 ++ A + N G D +KAA++ G Sbjct: 221 ASKAQILGLANAGEDTVNSIKAAKEFG 247 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 63/250 (25%), Positives = 109/250 (43%), Gaps = 19/250 (7%) Query: 46 VRIGMIDGLSGPFANAGEAVVRNISYAIERVNARGGVNLPDGKHLLQLSTFDNKLGVEDS 105 +RIG I+ + AG+A ++ A+E++NA GGV G LQL ++ + + Sbjct: 38 IRIGEINSRH-RLSTAGQAYRNGLALALEQINAAGGV----GGRPLQLVVREDDGDADQA 92 Query: 106 LVQFRQL-TDERIPFLIEGNSSAVAAALIDAVNKHNQREPDR-RVLFLNYSAVDPTLTNE 163 R L + + + L S VA A+ RE R R + + A+ +T + Sbjct: 93 ARNARALISQDHVEVLTGSMLSDVALAV--------SREAARGRTVLVVGDALSDAITLD 144 Query: 164 KCSFWHFRFDASADMRMQALTEAIKADPATKKVYLIGQDYSFGRQVAKAAREQITAKRPD 223 K + + FR S M+ L E P ++ ++ +Y +G+ + + + RPD Sbjct: 145 KGNRYTFRVRPSTYMQAAMLAEQAARLPG-RRWAVVAPNYEYGQSAVASFKLLLKQARPD 203 Query: 224 IAIVGDELHPIGKIKDFAPYIAK-MRSAGADAVITGNWGNDLTLLVKAARDAGFKAKFYT 282 + V D+ +GKIK A +A+ +R + D + +G DL V+A Sbjct: 204 VEFVSDQWPALGKIK--ADDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQLFEGVRV 261 Query: 283 FYANSLGAPA 292 S GAP+ Sbjct: 262 VSMASAGAPS 271 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 54/240 (22%), Positives = 104/240 (43%), Gaps = 24/240 (10%) Query: 46 VRIGMIDGLSGPFANAGEAVVRNISYAIERVNARGGVNLPDGKHLLQLSTFDNKLGVEDS 105 V+I + ++G A G+ V AIE++NA GG N + + D+ + + Sbjct: 29 VKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAGGAN----GNKFEAVMMDDACEPKQA 84 Query: 106 LVQFRQLTDERIPFLIEGNSSAVAAALIDAVNKHNQREPDRRVLFLNYSAVDPTLTNEKC 165 + ++ + I ++I + + I A + + + V+ + SA P LT K Sbjct: 85 VAVANKIVSQGIKYVI---GHVCSGSTIPASDIYE----NEGVVMITPSATAPQLTEAKP 137 Query: 166 SFWHFRFDASADMRMQALTEAIKADPATKKVYLIGQDYSFGRQVAKAAREQITAKRPDIA 225 + FR D + A + KKV ++ S+G+ VA + + + A + + Sbjct: 138 HKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVV 197 Query: 226 I-----VGDELHPIGKIKDFAPYIAKMRSAGADAVITGNWGNDLTLLVKAARDAGFKAKF 280 + GD D++ + K++S G D V G + ++ L+++ AR+ G KA F Sbjct: 198 VFEGINAGDS--------DYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVF 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 985,108 Number of extensions: 40131 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 6 Length of query: 346 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 260 Effective length of database: 1,212,660 Effective search space: 315291600 Effective search space used: 315291600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)