BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0140.p
         (256 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313462|ref|YP_003777554.1| hypothetical protein Hsero_4176...   428   e-121
gi|300313461|ref|YP_003777553.1| prolin-rich signal peptide prot...   169   3e-43

>gi|300313462|ref|YP_003777554.1| hypothetical protein Hsero_4176
           [Herbaspirillum seropedicae SmR1]
          Length = 253

 Score =  428 bits (1101), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 201/229 (87%), Positives = 217/229 (94%)

Query: 28  AADGPHPVDKRPFNLPPSAELHYAITAKQSGLDLKGEGLVKWTQSKTAYSVNTETRAMLL 87
           A DGPHPVDKR +NLPPSAEL+Y+I AKQSGLD+KG+GLVKWTQSKT+YSV +ETRAMLL
Sbjct: 25  ATDGPHPVDKRAYNLPPSAELNYSIQAKQSGLDVKGQGLVKWTQSKTSYSVTSETRAMLL 84

Query: 88  GKILETSSEGSIDAYGLAPSRFVEKRLRREPTTTTFNREQNKITFTESAESFPLQGGEQD 147
           GKILE +SEG++DAYGLAP RFVEKRLRREP+TTTFNREQNKITFT+SAES+PLQGGEQD
Sbjct: 85  GKILEATSEGAVDAYGLAPDRFVEKRLRREPSTTTFNREQNKITFTQSAESYPLQGGEQD 144

Query: 148 RTGITWQFVAIARANAAKMKPGSEWNFFVAGPRDAEQWTFKVVGREKIKTAQGETEALHL 207
           RT ITWQFVAIARANAAKMKPGSEWNFFVAG RDAEQWTFKVVGREKI+T QGETEALHL
Sbjct: 145 RTSITWQFVAIARANAAKMKPGSEWNFFVAGQRDAEQWTFKVVGREKIRTPQGETEALHL 204

Query: 208 FRNPPPDDQAQKLDIWLAPKMEWYPVRLKFTDPNGDYIEQVLDSVKKTN 256
           FR PPPDDQ QKLDIWLAPKMEWYPVRLKFTDP+GDYIEQ LDS+KKTN
Sbjct: 205 FRAPPPDDQGQKLDIWLAPKMEWYPVRLKFTDPDGDYIEQTLDSLKKTN 253


>gi|300313461|ref|YP_003777553.1| prolin-rich signal peptide protein
           [Herbaspirillum seropedicae SmR1]
          Length = 255

 Score =  169 bits (427), Expect = 3e-43,   Method: Compositional matrix adjust.
 Identities = 86/216 (39%), Positives = 123/216 (56%), Gaps = 3/216 (1%)

Query: 41  NLPPSAELHYAITAKQSGLDLKGEGLVKWTQSKTAYSVNTETRAMLLGKILETSSEGSID 100
           + PPSA L Y +T         G   + W  S   Y +  E  A+ +   L+ SSEG +D
Sbjct: 41  DAPPSAVLDYDVTGTIDQKPSYGSASLDWRNSGQHYRLEGEVTAIWV-TWLKFSSEGELD 99

Query: 101 AYGLAPSRFVEKRLRREPTTTTFNREQNKITFTESAESFPLQGGEQDRTGITWQFVAIAR 160
           A+G++P+ + EK+  R  T T FNR  + I+F+ S   +P +GGEQDR  + WQ  AI R
Sbjct: 100 AWGVSPTLYTEKKKFRSATNTHFNRNGDSISFSASETRYPRRGGEQDRGSLIWQLAAIGR 159

Query: 161 ANAAKMKPGSEWNFFVAGPRDAEQWTFKVVGREKIKTAQGETEALHLFRNPPPDDQAQKL 220
            + A+ +PG+  + FVAG RD E W  +V+G E IK   G  +  H+ R P P     ++
Sbjct: 160 GDPAQFQPGAVIDLFVAGVRDGEVWRMQVIGEEAIKLPIGTLQTWHVVRVPKPGAYEDRI 219

Query: 221 DIWLAPKMEWYPVRLKFTD--PNGDYIEQVLDSVKK 254
           DIWLAP+ EWYP RL++TD  PNGD  E  L  +++
Sbjct: 220 DIWLAPQHEWYPARLRYTDPRPNGDVTELSLSELRR 255


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.131    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 775,760
Number of extensions: 30768
Number of successful extensions: 64
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 63
Number of HSP's successfully gapped: 2
Length of query: 256
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 173
Effective length of database: 1,226,865
Effective search space: 212247645
Effective search space used: 212247645
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 129 (54.3 bits)