BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0137.p (798 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidogl... 1554 0.0 gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidogl... 855 0.0 gi|300312242|ref|YP_003776334.1| penicillin-binding 2 protein [H... 99 2e-21 gi|300309743|ref|YP_003773835.1| monofunctional biosynthetic pep... 80 4e-16 gi|300309672|ref|YP_003773764.1| cell division FtsI protein/peni... 57 5e-09 >gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 771 Score = 1554 bits (4023), Expect = 0.0, Method: Compositional matrix adjust. Identities = 753/771 (97%), Positives = 763/771 (98%) Query: 28 MHWSLRILLGLFGTVLGLVVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGV 87 MHWSLRILLGL GT+ GL VMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGV Sbjct: 1 MHWSLRILLGLVGTIFGLAVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGV 60 Query: 88 LIGEFGEERRNLVHFKDIPDIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGGGA 147 LIGEFGEERRNLVHFKDIP+IMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGGGA Sbjct: 61 LIGEFGEERRNLVHFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGGGA 120 Query: 148 STITQQVARNFFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILEVYMNQIYLGQR 207 STITQQVARNFFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILEVYMNQIYLGQR Sbjct: 121 STITQQVARNFFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILEVYMNQIYLGQR 180 Query: 208 AYGFASAAQIYFGKQLKDLTIAEAAMLAGLPKAPSAYNPVVNPKRAHARQQYILLRMRQL 267 AYGFASAAQIYFGKQLKDLTIAEAAMLAGLPKAPSAYNPVVNPKRAHARQQYILLRMRQL Sbjct: 181 AYGFASAAQIYFGKQLKDLTIAEAAMLAGLPKAPSAYNPVVNPKRAHARQQYILLRMRQL 240 Query: 268 GYITEAQYQEAKDEELRVKGDSSAFGMHAEYVAEMARQLVYAQYKDETYTRGLNVFTTIT 327 GYIT+AQYQ AKDEELRVKGDSSAFGMHAEYVAEMARQLVYAQYKDETYTRGLNVFTTIT Sbjct: 241 GYITDAQYQAAKDEELRVKGDSSAFGMHAEYVAEMARQLVYAQYKDETYTRGLNVFTTIT 300 Query: 328 KADQDAAYIALRRGIIDYERRHGYRGPEGYMELPEKATKEVVEDAVEVELADHPDSDDMV 387 K DQDAAYIALRRGIIDYERRHGYRGPEGYM+LPE ATK+ VEDAVEVELADHPDSDDMV Sbjct: 301 KTDQDAAYIALRRGIIDYERRHGYRGPEGYMDLPENATKDAVEDAVEVELADHPDSDDMV 360 Query: 388 AAVVLEAKPKEITAMLSTGEEISISGAGLGFGANLLSDKAPPQKKIRRGAIIRVFQDGNS 447 +AVVLEAKPKEITAMLSTGEEISISGAGLGFGANLLSDKAPPQKKIRRGAIIRVFQDGN+ Sbjct: 361 SAVVLEAKPKEITAMLSTGEEISISGAGLGFGANLLSDKAPPQKKIRRGAIIRVFQDGNN 420 Query: 448 WIITQMPEVESAFVSVNSRDGAIRSLVGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSL 507 WIITQMPEVESAFVSVNS+DGAIRSLVGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSL Sbjct: 421 WIITQMPEVESAFVSVNSQDGAIRSLVGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSL 480 Query: 508 EKGLSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVG 567 EKGLSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVG Sbjct: 481 EKGLSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVG 540 Query: 568 VKYAQEYITRFGFDADKNPPYLTLALGAGNVTPLQMVGAYSVFANGGYKINPYLITKVTD 627 VKYAQEYITRFGFDADKNPPYLTLALGAGNVTPLQMVGAYSVFANGGYKINPYLITKVTD Sbjct: 541 VKYAQEYITRFGFDADKNPPYLTLALGAGNVTPLQMVGAYSVFANGGYKINPYLITKVTD 600 Query: 628 SNGEVLSQAKPATAEDESNRVIDARNAFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGT 687 SNGEVLSQAKPATA+DESNRVIDARNAFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGT Sbjct: 601 SNGEVLSQAKPATADDESNRVIDARNAFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGT 660 Query: 688 TNDSMDAWFAGYQPGLTAIAWIGYDQPKSLGDRETGGGLALPVWISYMSKALKDVPNVDR 747 TNDSMDAWFAGYQPGLTAIAWIGYDQPKSLGDRETGGGLALPVWISYMSKALKDVPNVDR Sbjct: 661 TNDSMDAWFAGYQPGLTAIAWIGYDQPKSLGDRETGGGLALPVWISYMSKALKDVPNVDR 720 Query: 748 KVPDGVIESGGEYYYAEFPPSAMVRDLGMGDRGGLTPEEEKAKTQMKNELF 798 KVPDG+IESGGEYYYAEFPPSAMVRDLGMGDR GLTPEEEKAKTQMKNELF Sbjct: 721 KVPDGLIESGGEYYYAEFPPSAMVRDLGMGDRSGLTPEEEKAKTQMKNELF 771 >gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 822 Score = 855 bits (2208), Expect = 0.0, Method: Compositional matrix adjust. Identities = 433/742 (58%), Positives = 564/742 (76%), Gaps = 15/742 (2%) Query: 41 TVLGLVVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGVLIGEFGEERRNLV 100 ++LGL V L V + + P+LP L+ +TDYRPK+PLRI+S D VL+GEFGEE+R+LV Sbjct: 32 SILGLGVAGALLVVYALIFINPRLPSLELITDYRPKVPLRIWSEDKVLLGEFGEEKRSLV 91 Query: 101 HFKDIPDIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGG--GASTITQQVARNF 158 DIP MK AVLAIED RFYEHGG+D+ GIARA +++ G GASTIT QVARN Sbjct: 92 KLDDIPADMKNAVLAIEDYRFYEHGGIDWIGIARALVTDVLRGSASQGASTITMQVARNV 151 Query: 159 FLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILEVYMNQIYLGQRAYGFASAAQIY 218 FLT E TF +K YE+ LA KIE+ LTKDQILEVYMNQIYLGQRA+GFA+AA+ Y Sbjct: 152 FLTK-EKTFSRK-----LYEVLLANKIEHALTKDQILEVYMNQIYLGQRAFGFAAAARTY 205 Query: 219 FGKQLKDLTIAEAAMLAGLPKAPSAYNPVVNPKRAHARQQYILLRMRQLGYITEAQYQEA 278 FGK LKD+T+AEAAMLAGLPKAPSAYNP+VNPKRAH RQQYIL RM +G+IT+AQY++A Sbjct: 206 FGKDLKDVTLAEAAMLAGLPKAPSAYNPIVNPKRAHIRQQYILQRMLDVGFITKAQYEKA 265 Query: 279 KDEELRVKGDSSAFGMHAEYVAEMARQLVYAQYKDETYTRGLNVFTTITKADQDAAYIAL 338 EEL+V+ S + +HAEYV EM RQ+VYAQYK++TYT+G NV+TT+ ADQ AAY+A+ Sbjct: 266 AKEELKVRAPGSQYNIHAEYVNEMVRQMVYAQYKEDTYTQGFNVYTTVNAADQQAAYLAV 325 Query: 339 RRGIIDYERRHGYRGPEGYMELPEKATKEVVEDAVEVELADHPDSDDMVAAVVLEAKPKE 398 R+G++DY+RRHGYRGPE +ELP A A++ + DHPD+DD++AAVV+ A PK+ Sbjct: 326 RKGVMDYDRRHGYRGPEETIELPANAEDRAA--AIDDVMDDHPDNDDILAAVVVAASPKQ 383 Query: 399 ITAMLSTGEEISISGAGLGFGANLLSDKAPPQKKIRRGAIIRVFQDG-NSWIITQMPEVE 457 I A+L +G++ISI G L F A+ LS+KA +I GA+IRV QD +W + Q+P+VE Sbjct: 384 IDAVLRSGDKISIKGEALRFVASALSEKAKKDIRIGPGALIRVMQDAKQNWQVAQLPQVE 443 Query: 458 SAFVSVNSRDGAIRSLVGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSLEKGLSPATII 517 +A VSV +DGAIR+LVGGFDF +N FNHVTQAWRQPGS+FKPFIYS++LEKG +PA+I+ Sbjct: 444 AALVSVVPQDGAIRALVGGFDFTQNNFNHVTQAWRQPGSTFKPFIYSAALEKGFAPASIV 503 Query: 518 NDAPLTFTQTGGQV-WQPKNYGGKFEGPISMRRALQKSINLVSIRILERVGVKYAQEYIT 576 NDAP+++ GQ W+P++ GPI++R ALQKS+NLV+IR L+ +GV YA+++IT Sbjct: 504 NDAPVSYPAGPGQPNWEPRD-DETPAGPITVRTALQKSVNLVAIRTLDYIGVPYAKDFIT 562 Query: 577 -RFGFDADKNPPYLTLALGAGNVTPLQMVGAYSVFANGGYKINPYLITKVTDSNGEVLSQ 635 +GFDADK PPYL +ALGAG PLQ+ AYSVFANGGYK+ PYLI+++TD+ G+ LS+ Sbjct: 563 GTYGFDADKVPPYLPMALGAGQTNPLQLSAAYSVFANGGYKVQPYLISQITDARGQALSK 622 Query: 636 AKPATAEDESNRVIDARNAFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGTTNDSMDAW 695 A+P + + RV+ RN+++M SML+ V + GT ++ S+ R+DLAGKTGTTND++D W Sbjct: 623 AEPLIVGNGAPRVVSERNSYMMTSMLKSVAQGGTGARSNSIGRSDLAGKTGTTNDAVDGW 682 Query: 696 FAGYQPGLTAIAWIGYDQPKSLGDRETGGGLALPVWISYMSKALKDVPNVDRKVPDGVIE 755 FAGYQ L + W+GYDQPKSLG +E G LALP+W+ YM+KALK VP D VP+G+ Sbjct: 683 FAGYQRTLATVVWMGYDQPKSLGSKEFGAQLALPIWVDYMAKALKGVPQFDLPVPEGIRF 742 Query: 756 SGGEYYYAEFPP-SAMVRDLGM 776 GE YY F P + +V ++G+ Sbjct: 743 IDGEPYYDNFTPNNGLVTNVGV 764 >gi|300312242|ref|YP_003776334.1| penicillin-binding 2 protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 98.6 bits (244), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 81/252 (32%), Positives = 129/252 (51%), Gaps = 22/252 (8%) Query: 490 AWRQPGSSFKPFIYSSSLEKG-LSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMR 548 A RQ GS+ KPF+Y ++EK L+ A++++D+P+ + G ++ P+NY +++G +S+R Sbjct: 341 AQRQAGSTLKPFLYELAIEKKWLTAASLLDDSPVNLATSAG-LYIPQNYDKQYKGLVSLR 399 Query: 549 RALQKSINLVSIRILERVGVKYAQEYITRFGFDADKNPPY--LTLALGAGNVTPLQMVGA 606 +L S+N+ ++R L V + + GF+ ++ Y +LALG+ +VT + A Sbjct: 400 TSLGSSLNIPAVRTLVMVTPERFFRRLQALGFNLRESGDYYGYSLALGSADVTLANLANA 459 Query: 607 YSVFANGGYKINPYLITKVTDSNGEVLSQAKPATAEDESNRVIDARNAFIMDSMLRD--- 663 Y V AN G + P L T++ ++ PA A +V+DA A+I+ +L D Sbjct: 460 YRVLANQG-RYTP-LRTRLDEAR-------TPARA----TQVMDADAAYIVGDILSDRSA 506 Query: 664 VVRHGTAVKALSLKRTDLAGKTGTTNDSMDAWFAGYQPGLTAIAWIGYDQPKSLGDRETG 723 R ALS R A KTGT+ D D W GY T W+G + D +G Sbjct: 507 RARTFGLENALS-TRIWTAVKTGTSKDMRDNWCVGYSTRYTVGVWVGNASGAPMWD-VSG 564 Query: 724 GGLALPVWISYM 735 A PVW M Sbjct: 565 VTGAAPVWQEVM 576 Score = 85.9 bits (211), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 55/172 (31%), Positives = 85/172 (49%), Gaps = 13/172 (7%) Query: 94 EERRNLVHFKDIPDIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNL-SGGGGGASTITQ 152 E + V +D+ ++ A++A ED RFY+H G+D+ +A + + NL + GASTIT Sbjct: 61 ERKLAWVRLEDVSPALRHALIASEDRRFYQHSGIDWSAVAASAWGNLWNSKTRGASTITM 120 Query: 153 QVARNFFLTSNEPTFLQKATRKFFYEIPLAW---KIENNLTKDQILEVYMNQIYLGQRAY 209 Q+A + + + R ++ AW +E N KDQILE Y+N + Sbjct: 121 QLAGLIY----DDLRRKSGARSLTQKVSQAWIAQSLERNWRKDQILEAYLNLVAFRGELV 176 Query: 210 GFASAAQIYFGKQLKDLTIAEAAMLAGLPKAPSAYNPVVNPKRAHARQQYIL 261 G + ++I FGK L EAA+ L +AP+A P+R R IL Sbjct: 177 GVHALSRIMFGKHPSGLDQGEAAIAVALIRAPNA-----TPRRVADRACAIL 223 >gi|300309743|ref|YP_003773835.1| monofunctional biosynthetic peptidoglycan transglycosylase [Herbaspirillum seropedicae SmR1] Length = 235 Score = 80.5 bits (197), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 57/167 (34%), Positives = 85/167 (50%), Gaps = 8/167 (4%) Query: 100 VHFKDIPDIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGG--GGASTITQQVARN 157 V + I + +K+A++A ED F H GVD++ + +A N G G STITQQ+A+N Sbjct: 65 VPYARISNNLKRAIIASEDSNFSSHDGVDWEALQKAYEKNEKKGKVVAGGSTITQQLAKN 124 Query: 158 FFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILEVYMNQIYLGQRAYGFASAAQI 217 FL S E ++L+K E + + +E L K +I E+Y+N + G +G +AAQ Sbjct: 125 LFL-SGERSYLRKGQ-----EFIITYMLEFLLDKQRIFEIYLNVVEWGNGVFGAEAAAQH 178 Query: 218 YFGKQLKDLTIAEAAMLAGLPKAPSAYNPVVNPKRAHARQQYILLRM 264 Y+ L +AA LA + P Y+ AR IL RM Sbjct: 179 YYRIPAASLGAEQAARLAVMLPRPRYYDRNRGSGYLAARTSLILRRM 225 >gi|300309672|ref|YP_003773764.1| cell division FtsI protein/penicillin-binding protein 2 [Herbaspirillum seropedicae SmR1] Length = 592 Score = 57.4 bits (137), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 80/321 (24%), Positives = 129/321 (40%), Gaps = 63/321 (19%) Query: 460 FVSVNSRDGAIRSLVGGFDFNRNKF---------NHVTQAWRQPGSSFKPFIYSSSLEKG 510 + V+++ G + +L +N N N V +PGS+ KPF + +L+ Sbjct: 271 IIVVDAKTGEVLALANLPTYNPNDRSVLTGAQLRNRVVTDTFEPGSTMKPFTVALALDTH 330 Query: 511 -LSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVGVK 569 ++P+T+ AP T + +G P+++ + ++KS N+ + +I + + Sbjct: 331 RVTPSTVFQTAPGKMTIGTATIGDSHAHG-----PLTVAQIIEKSSNIGTAKIALGMPPE 385 Query: 570 YAQEYITRFGFD-----------ADKNPPY--------LTLALGAG-NVTPLQMVGAYSV 609 E T GF A + PY T++ G G +V+ +Q+ AY + Sbjct: 386 EMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRPIEQATMSYGHGISVSLIQLAHAYLI 445 Query: 610 FANGGYKINPYLITKVTDSNGEVLSQAKPATAEDESNRVIDARNAFIMDSMLRDVVRHGT 669 FA G I P TKVTDS RVI A A M ML VV G Sbjct: 446 FARNG-DIIPLSFTKVTDS--------------PIGQRVISADTALQMRRMLETVVAPGG 490 Query: 670 AVKALSLKRTDLAGKTGTTND--------SMDAWFAGYQP--GLTAIAWIGYDQPKSLGD 719 + + GKTGT A F+G P I + D+P+ Sbjct: 491 TAPQAQVPGYRVGGKTGTAYKIEGGKYVRKYVASFSGIAPMSDPRLIISVMIDEPQG--- 547 Query: 720 RETGGGLALPVWISYMSKALK 740 + GG +A PV+ + + AL+ Sbjct: 548 QHYGGPVAGPVFANVAANALR 568 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,539,532 Number of extensions: 109539 Number of successful extensions: 334 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 324 Number of HSP's successfully gapped: 6 Length of query: 798 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 705 Effective length of database: 1,179,515 Effective search space: 831558075 Effective search space used: 831558075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)