BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0133.p (215 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313468|ref|YP_003777560.1| hypothetical protein Hsero_4182... 85 6e-18 >gi|300313468|ref|YP_003777560.1| hypothetical protein Hsero_4182 [Herbaspirillum seropedicae SmR1] Length = 202 Score = 84.7 bits (208), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 60/160 (37%), Positives = 89/160 (55%), Gaps = 10/160 (6%) Query: 27 PDEVQATRMWITQVRPSSTLP--LPAAALSDAATEDSLPG-EDSELPDPFQL---GALAP 80 PDE+ +TR W+ R S+ P A+L AA+E P D E+ DPF+L A P Sbjct: 26 PDELASTRQWLAGARQLSSQAGAAPPASLVQAASETPAPPLPDVEIVDPFRLRQDAAAPP 85 Query: 81 ASPTRPAASRVRAQDEEDEENSPVPAAAPIAPLPTPVLRLLGTLRRDQEWIAIAELEGST 140 A+ RVR E+E++ PVP+ P APLP RLLGTLR+ +A+ ++ G+T Sbjct: 86 ATAVERPPQRVRRDASEEEDDEPVPSPRPTAPLP----RLLGTLRQQDLMLALIDIGGAT 141 Query: 141 RQLQTGDTLPGGLGRVLAVREDALDIGQDDAARQTIAMTT 180 +LQ G LP G G++L + E ++I RQT+++ + Sbjct: 142 HRLQAGQQLPDGQGQLLTISETHIEIADPQGTRQTLSLES 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 473,618 Number of extensions: 15600 Number of successful extensions: 93 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 90 Number of HSP's successfully gapped: 1 Length of query: 215 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 134 Effective length of database: 1,236,335 Effective search space: 165668890 Effective search space used: 165668890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)